The hexosaminohexuronide of the bovine lens capsule

Dische, Zacharias; Zelmanis, Ginevra; Rothschild, Carl

doi:10.1016/0003-9861(67)90054-9

Cited by 18 publications

(3 citation statements)

References 14 publications

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“…Although the carbohydrate composition of lens capsule was reported earlier (Pirie, 1951;Dische and Borenfreund, 1954;Dische, 1964;Dische et al, 1967), no data were available on the complete amino acid composition of this structure. At the time this paper was submitted for publication, Spiro (1969) reported on the chemistry of bovine lens capsule.…”

Section: Discussionmentioning

confidence: 96%

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Structural components of epithelial and endothelial basement membranes

Kefalides¹,

Denduchis²

1969

Biochemistry

167

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Basement membranes from vascular and nonvascular canine tissue were analyzed chemically and structurally. Basement membranes which derive from different embryologic cell lines were qualitatively similar but differed quantitatively in their amino acid and carbohydrate composition. In terms of residues per 1000 residues anterior lens capsule contained hydroxylysine 32, hydroxyproline 99.5, and glycine 288, compared with 20, 83.8, and 230 residues, respectively, for Descemet's membrane. Glomerular, alveolar, and choroid plexus basement membranes contained hydroxylysine 24, 6.8, and 9 residues, hydroxyproline 65, 35, and 48 residues, and glycine 229, 222, and 235 residues, respectively. The carbohydrate content was high in all, ranging from 12.5 and, 10.5% in anterior lens capsule and Descemet's membrane to 10.25, 6.7, and 7.3% in basement membranes from the glomerulus, alveolus, and choroid

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Section: Discussionmentioning

confidence: 96%

“…Total protein-bound hexose was determined by the orcinol reaction of Weimer and Moshin (1953). Fucose was measured by the method of Dische and Shettles (1948). Glucosamine and galactosamine were determined on protein samples, that were hydrolyzed in 6 n HC1 for 4 hr at 100°, using the Beckman Model 116 amino acid analyzer.…”

Section: Methodsmentioning

confidence: 99%

Structural components of epithelial and endothelial basement membranes

Kefalides¹,

Denduchis²

1969

Biochemistry

167

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“…The initial identification of the glycosaminoglycan heparan sulphate in rabbit and bovine lens capsule by Dische (13,14) has since been confirmed in the bovine by others (15,16). Moczar et al (17) have also shown that cultured bovine lens epithelial cells can synthesize heparan sulphate and other sulphated glycosaminoglycans.…”

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confidence: 94%

Biosynthesis of sulphated macromolecules by rabbit lens epithelium. I. Identification of the major macromolecules synthesized by lens epithelial cells in vitro.

Heathcote¹,

Orkin²

1984

The Journal of Cell Biology

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Rabbit lens epithelial cells synthesize and secrete a variety of [35S]sulphate-labeled glycoconjugates in vitro. Associated with the cell layer, and with the medium, was a high molecular weight glycoconjugate(s) that contained heparan sulphate which was apparently covalently linked to sulphated glycoprotein. This component(s) was eluted in the void volume of a Sepharose CL-2B column and could not be fractionated by detergent treatment or extraction with lipid solvents. The cell layer also contained glycosaminoglycans (72% heparan sulphate, 28% chondroitin sulphate), as well as a small proportion of a low molecular weight sulphated glycoprotein. The major 3SS-labeled species secreted into the medium were sulphated glycoproteins with approximate molecular weights of 120,000 and 35,000 together with a heparan sulphate proteoglycan. This proteoglycan could be precipitated from the culture medium with 30% saturated (NH4)2SO4 and eluted from Sepharose CL-4B columns at approximately the same position (Kay = 0.15) as heparan sulphate proteoglycans described in the basement membrane of the EHS "sarcoma"

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