The hidden RNA code: implications of the RNA epitranscriptome in the context of viral infections

Ribeiro, Diana Roberta; Nunes, Alexandre; Ribeiro, Daniela; Soares, Ana Raquel

doi:10.3389/fgene.2023.1245683

Cited by 1 publication

(1 citation statement)

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“…As such, it is not known if genomic RNA receives a uniform modification pattern or if there are differences between individual molecules. RNA modification is known to alter secondary structures and can also impact the binding affinity of RNA-binding proteins [ 135 ]. In theory, distinctive modification patterns could impact protein binding and offer a potential mechanism for the differential trafficking of positive-strand viral RNA molecules destined for translation, replication, or virion assembly [ 43 ].…”

Section: Important Structural Features Of Flavivirus Rnamentioning

confidence: 99%

The Dynamic Landscape of Capsid Proteins and Viral RNA Interactions in Flavivirus Genome Packaging and Virus Assembly

Jablunovsky,

Jose

2024

Pathogens

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The flavivirus genus of the Flaviviridae family of enveloped single-stranded RNA viruses encompasses more than 70 members, many of which cause significant disease in humans and livestock. Packaging and assembly of the flavivirus RNA genome is essential for the formation of virions, which requires intricate coordination of genomic RNA, viral structural, and nonstructural proteins in association with virus-induced, modified endoplasmic reticulum (ER) membrane structures. The capsid (C) protein, a small but versatile RNA-binding protein, and the positive single-stranded RNA genome are at the heart of the elusive flavivirus assembly process. The nucleocapsid core, consisting of the genomic RNA encapsidated by C proteins, buds through the ER membrane, which contains viral glycoproteins prM and E organized as trimeric spikes into the lumen, forming an immature virus. During the maturation process, which involves the low pH-mediated structural rearrangement of prM and E and furin cleavage of prM in the secretory pathway, the spiky immature virus with a partially ordered nucleocapsid core becomes a smooth, mature virus with no discernible nucleocapsid. This review focuses on the mechanisms of genome packaging and assembly by examining the structural and functional aspects of C protein and viral RNA. We review the current lexicon of critical C protein features and evaluate interactions between C and gRNA in the context of assembly and throughout the life cycle.

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Section: Important Structural Features Of Flavivirus Rnamentioning

confidence: 99%