The high-affinity metal Transporters NRAMP1 and IRT1 Team up to Take up Iron under Sufficient Metal Provision

Castaings, Loren; Caquot, Antoine; Loubet, Stéphanie; Curie, Catherine

doi:10.1038/srep37222

Cited by 144 publications

(114 citation statements)

References 42 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…NRAMPs are suggested to have a broad range of metal substrates (Nevo & Nelson, ; Socha & Guerinot, ). Consistently, NRAMP1 is reported to be able to take up both Mn and Fe in Arabidopsis (Cailliatte et al ., ; Castaings et al ., ). NRAMP3 and NRAMP4 are required for the remobilization of both vacuolar Fe and Mn under Fe‐ or Mn‐deficient conditions (Lanquar et al ., , ).…”

Section: Discussionmentioning

confidence: 97%

“…Recently, Castaings et al . () demonstrated that NRAMP1 and IRT1 are cooperatively required for Mn and Fe uptake. Both NRAMP3 and NRAMP4 are localized at the vacuolar membrane and play redundant roles in the remobilization of the vacuolar Fe pool for proper plant growth during seed germination and early seedling stage under Fe‐deficient conditions (Lanquar et al ., ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

NRAMP2, a trans‐Golgi network‐localized manganese transporter, is required for Arabidopsis root growth under manganese deficiency

et al. 2017

View full text Add to dashboard Cite

SummaryTo cope with manganese (Mn) deficiency, plants have evolved an efficient transport system to uptake and redistribute Mn. However, the underlying molecular mechanisms remain to be demonstrated.We carried out a forward genetic screen in a root high-affinity Mn transporter nramp1 mutant background in Arabidopsis thaliana and identified an uncharacterized Mn transport NRAMP2. We investigated the effect of nramp2 mutation on root growth and reactive oxygen species (ROS) accumulation and we also examined the NRAMP2 expression pattern, and the subcellular localization and transport activity of NRAMP2.Mutation of NRAMP2 impaired plant growth, while overexpression of NRAMP2 improved plant growth under low Mn conditions. In the nramp2-1nramp1 double mutant, Mn deficiency inhibited root cell elongation and root hair development, which was associated with increased hydrogen peroxide (H 2 O 2 ) accumulation. NRAMP2 is preferentially localized to the trans-Golgi network. NRAMP2 has Mn influx transport activity in yeast, and mutation of NRAMP2 led to greater Mn retention in roots.Our results suggest that under Mn-deficient conditions, increased accumulation of H 2 O 2 is partially responsible for the root growth inhibition and NRAMP2 is involved in remobilization of Mn in Golgi for root growth.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

NRAMP2, a trans‐Golgi network‐localized manganese transporter, is required for Arabidopsis root growth under manganese deficiency

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Determination of the function and sub-cellular localisation of these transporters will be essential to test this model. The identity of the transporter that is responsible for xylem loading of Ni is unknown, but a putative homologue of NRAMP1, recently shown to mediate Fe transport across the endodermis (Castaings et al, 2016), is expressed at higher levels in roots of hyperaccumulators ( Figure 4), and may be worthy of further investigation. Finally, given the increased expression of putative IRT1 and IREG2 homologues reported in N. caerulescens and P. gabriellae it is possible that a similar mechanism might also operate in these Ni hyperaccumulators.…”

Section: Senecio Coronatus Hyperaccumulators May Have Enhanced Capacimentioning

confidence: 99%

Comparative RNA‐seq analysis of nickel hyperaccumulating and non‐accumulating populations of Senecio coronatus (Asteraceae)

et al. 2018

View full text Add to dashboard Cite

Most metal hyperaccumulating plants accumulate nickel, yet the molecular basis of Ni hyperaccumulation is not well understood. We chose Senecio coronatus to investigate this phenomenon as this species displays marked variation in shoot Ni content across ultramafic outcrops in the Barberton Greenstone Belt (South Africa), thus allowing an intraspecific comparative approach to be employed. No correlation between soil and shoot Ni contents was observed, suggesting that this variation has a genetic rather than environmental basis. This was confirmed by our observation that the accumulation phenotype of plants from two hyperaccumulator and two non-accumulator populations was maintained when the plants were grown on a soil mix from these four sites for 12 months. We analysed the genetic variation among 12 serpentine populations of S. coronatus, and used RNA-seq for de novo transcriptome assembly and analysis of gene expression in hyperaccumulator versus non-accumulator populations. Genetic analysis revealed the presence of hyperaccumulators in two well supported evolutionary lineages, indicating that Ni hyperaccumulation may have evolved more than once in this species. RNA-Seq analysis indicated that putative homologues of transporters associated with root iron uptake in plants are expressed at elevated levels in roots and shoots of hyperaccumulating populations of S. coronatus from both evolutionary lineages. We hypothesise that Ni hyperaccumulation in S. coronatus may have evolved through recruitment of these transporters, which play a role in the iron-deficiency response in other plant species.

show abstract

“…It is possible that other unspecific metal transporters, highly expressed under low external Fe concentrations and derepressed in the absence of IRT1, are responsible for Mn-induced Fe deficiency. The most likely candidate appears to be NRAMP1, which facilitates the uptake not only of Mn but also of Fe (Cailliatte et al, 2010;Castaings et al, 2016).…”

Section: Excess Mn Provokes Fe Deficiency In the Absence Of Irt1mentioning

confidence: 99%

Heavy Metals Induce Iron Deficiency Responses at Different Hierarchic and Regulatory Levels

et al. 2017

View full text Add to dashboard Cite

In plants, the excess of several heavy metals mimics iron (Fe) deficiency-induced chlorosis, indicating a disturbance in Fe homeostasis. To examine the level at which heavy metals interfere with Fe deficiency responses, we carried out an in-depth characterization of Fe-related physiological, regulatory, and morphological responses in Arabidopsis (Arabidopsis thaliana) exposed to heavy metals. Enhanced zinc (Zn) uptake closely mimicked Fe deficiency by leading to low chlorophyll but high ferric-chelate reductase activity and coumarin release. These responses were not caused by Zn-inhibited Fe uptake via IRON-REGULATED TRANSPORTER (IRT1). Instead, Zn simulated the transcriptional response of typical Fe-regulated genes, indicating that Zn affects Fe homeostasis at the level of Fe sensing. Excess supplies of cobalt and nickel altered root traits in a different way from Fe deficiency, inducing only transient Fe deficiency responses, which were characterized by a lack of induction of the ethylene pathway. Cadmium showed a rather inconsistent influence on Fe deficiency responses at multiple levels. By contrast, manganese evoked weak Fe deficiency responses in wild-type plants but strongly exacerbated chlorosis in irt1 plants, indicating that manganese antagonized Fe mainly at the level of transport. These results show that the investigated heavy metals modulate Fe deficiency responses at different hierarchic and regulatory levels and that the interaction of metals with physiological and morphological Fe deficiency responses is uncoupled. Thus, this study not only emphasizes the importance of assessing heavy metal toxicities at multiple levels but also provides a new perspective on how Fe deficiency contributes to the toxic action of individual heavy metals.

show abstract

The high-affinity metal Transporters NRAMP1 and IRT1 Team up to Take up Iron under Sufficient Metal Provision

Cited by 144 publications

References 42 publications

NRAMP2, a trans‐Golgi network‐localized manganese transporter, is required for Arabidopsis root growth under manganese deficiency

NRAMP2, a trans‐Golgi network‐localized manganese transporter, is required for Arabidopsis root growth under manganese deficiency

Comparative RNA‐seq analysis of nickel hyperaccumulating and non‐accumulating populations of Senecio coronatus (Asteraceae)

Heavy Metals Induce Iron Deficiency Responses at Different Hierarchic and Regulatory Levels

Contact Info

Product

Resources

About