The high‐light‐induced protein <scp>SliP4</scp> binds to <scp>NDH1</scp> and photosystems facilitating cyclic electron transport and state transition in <i>Synechocystis</i> sp. PCC 6803

Alvarenga-Lucius, Luna; Linhartová, Markéta; Schubert, Hendrik; Maaß, Sandra; Becher, Dörte; Hess, Wolfgang R.; Sobotka, Roman; Hagemann, Martin

doi:10.1111/nph.18987

Cited by 4 publications

(1 citation statement)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Coimmunoprecipitation (co-IP) was successfully used in the past to identify the interacting partners of Synechocystis 6803 small proteins NblD, AtpΘ, and SliP4, thereby providing essential hints for their specific functions [25,26,37]. Therefore, we used a strain expressing epitope-tagged NirP1 to obtain insight into the molecular mechanism that involves NirP1.…”

Section: Nirp1 Interacts With Ferredoxin-nitrite Reductasementioning

confidence: 99%

Nitrite excretion by cyanobacteria is controlled by the small protein NirP1

Hess,

Kraus,

Spät

et al. 2023

Preprint

View full text Add to dashboard Cite

When the supply of inorganic carbon is limiting, photosynthetic cyanobacteria excrete nitrite, a toxic intermediate in the ammonia assimilation pathway from nitrate. While it has been hypothesized that the excreted nitrite represents excess nitrogen that could not be further assimilated due to the missing carbon, the underlying molecular mechanism has remained enigmatic. Here, we characterized a hitherto unannotated gene with homologs in the genomes of 485 different cyanobacteria that is upregulated under low carbon conditions and controlled by the transcription factor NtcA, a central regulator of nitrogen homeostasis. To understand its function, we ectopically overexpressed it in Synechocystis sp. PCC 6803, which resulted in a chlorotic phenotype, delayed growth, severe changes in amino acid pools, and nitrite excretion. Coimmunoprecipitation experiments revealed that this protein targets nitrite reductase, a central enzyme in the assimilation of ammonia from nitrate/nitrite, and was re-named to nitrite reductase regulator protein 1 (NirP1). Our results reveal that NirP1 is widely conserved in cyanobacteria and plays a crucial role in the coordination of C/N primary metabolism by targeting one of the central enzymes. In natural environments, the excreted nitrite will be utilized by other microorganisms; therefore, NirP1 ultimately impacts the activities and composition of the surrounding microbiome.

show abstract

Section: Nirp1 Interacts With Ferredoxin-nitrite Reductasementioning

confidence: 99%

Nitrite excretion by cyanobacteria is controlled by the small protein NirP1

Hess,

Kraus,

Spät

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

How Small Proteins Adjust the Metabolism of Cyanobacteria Under Stress

Kraus,

Hess

2024

BioEssays

View full text Add to dashboard Cite

Several recently discovered small proteins of less than 100 amino acids control important, but sometimes surprising, steps in the metabolism of cyanobacteria. There is mounting evidence that a large number of small protein genes have also been overlooked in the genome annotation of many other microorganisms. Although too short for enzymatic activity, their functional characterization has frequently revealed the involvement in processes such as signaling and sensing, interspecies communication, stress responses, metabolism, regulation of transcription and translation, and in the formation of multisubunit protein complexes. Cyanobacteria are the only prokaryotes that perform oxygenic photosynthesis. They thrive under a wide variety of conditions as long as there is light and must cope with dynamic changes in the environment. To acclimate to these fluctuations, frequently small regulatory proteins become expressed that target key enzymes and metabolic processes. The consequences of their actions are profound and can even impact the surrounding microbiome. This review highlights the diverse functions of recently discovered small proteins that control cyanobacterial metabolism. It also addresses why many of these proteins have been overlooked so far and explores the potential for implementing metabolic engineering strategies to improve the use of cyanobacteria in biotechnological applications.

show abstract

Protein NirP1 regulates nitrite reductase and nitrite excretion in cyanobacteria

Kraus,

Spät,

Timm

et al. 2024

Nat Commun

View full text Add to dashboard Cite

When the supply of inorganic carbon is limiting, photosynthetic cyanobacteria excrete nitrite, a toxic intermediate in the ammonia assimilation pathway from nitrate. It has been hypothesized that the excreted nitrite represents excess nitrogen that cannot be further assimilated due to the missing carbon, but the underlying molecular mechanisms are unclear. Here, we identified a protein that interacts with nitrite reductase, regulates nitrogen metabolism and promotes nitrite excretion. The protein, which we named NirP1, is encoded by an unannotated gene that is upregulated under low carbon conditions and controlled by transcription factor NtcA, a central regulator of nitrogen homeostasis. Ectopic overexpression of nirP1 in Synechocystis sp. PCC 6803 resulted in a chlorotic phenotype, delayed growth, severe changes in amino acid pools, and nitrite excretion. Coimmunoprecipitation experiments indicated that NirP1 interacts with nitrite reductase, a central enzyme in the assimilation of ammonia from nitrate/nitrite. Our results reveal that NirP1 is widely conserved in cyanobacteria and plays a crucial role in the coordination of C/N primary metabolism by targeting nitrite reductase.

show abstract

The high‐light‐induced protein SliP4 binds to NDH1 and photosystems facilitating cyclic electron transport and state transition in Synechocystis sp. PCC 6803

Cited by 4 publications

References 60 publications

Nitrite excretion by cyanobacteria is controlled by the small protein NirP1

Nitrite excretion by cyanobacteria is controlled by the small protein NirP1

How Small Proteins Adjust the Metabolism of Cyanobacteria Under Stress

Protein NirP1 regulates nitrite reductase and nitrite excretion in cyanobacteria

Contact Info

Product

Resources

About