The high-resolution G-banded karyotype of Sus scrofa domestica L

Galman, O; Yerle, Martine M.; Echard, G.

doi:10.1186/1297-9686-23-s1-s113

Cited by 1 publication

(2 citation statements)

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“…Conventional cytogenetic diagnostics are based on the microscopic analysis of metaphase chromosomes obtained from in vitro lymphocyte culture that is nondifferentially stained with Giemsa, which allows the determination of the diploid number (2n = 38) and morphology of chromosomes (submetacentric, metacentric, subtelocentric, and telocentric) ( Figure 1). Standard cytogenetic analysis is extended with high-resolution banding techniques (HRBTs) (resolution 2-5 Mb), which yield 600 G-or R-bands or sub-bands in the haploid set of elongated prometaphase chromosomes (obtained using replication and condensation blockers), corresponding to the standard karyotype for prometaphase chromosomes of the domestic pig [62,63].…”

Section: Cytomolecular Diagnosticsmentioning

confidence: 99%

“…This method also allows for interspecific in situ hybridizations (Zoo-FISH), where human probes are used to diagnose genetic defects or karyotype changes in different species of animals [67][68][69][70][71][72][73]. Standard cytogenetic analysis is extended with high-resolution banding techniques (HRBTs) (resolution 2-5 Mb), which yield 600 G-or R-bands or sub-bands in the haploid set of elongated prometaphase chromosomes (obtained using replication and condensation blockers), corresponding to the standard karyotype for prometaphase chromosomes of the domestic pig [62,63].…”

Section: Cytomolecular Diagnosticsmentioning

confidence: 99%

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Breeding and Economic Aspects of Cytogenetic Screening Studies of Pigs Qualified for Reproduction

Danielak-Czech

Kozubska‐Sobocińska

Smołucha

et al. 2020

Animals

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Cytogenetic monitoring allows the identification and early removal of pigs affected by inherited karyotype defects from breeding herds. These abnormalities cause developmental anomalies, considerably reducing the fertility (by several dozen to 100%) and performance parameters of breeding herds, resulting in substantial financial losses. This mainly concerns reciprocal translocations, typical of pigs, which are highly prevalent (about 0.46%), generally occur de novo, and normally result in low breeding soundness of the carriers. Due to the potential spontaneous occurrence of chromosomal aberrations and the rapid spread of these genetic defects in the population, especially under artificial insemination conditions, it is necessary to perform routine karyotype screening of animals qualified for reproduction. The cytogenetic screening program for young boars, carried out using continually refined diagnostic techniques, permits a precise and reliable karyotype assessment, identification of chromosomal abnormalities, and formulation of specific selection guidelines.

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Section: Cytomolecular Diagnosticsmentioning

confidence: 99%