The high-resolution GTG-banding pattern of pig chromosomes

Yerle, Martine M.; Galman, O; Echard, G.

doi:10.1159/000133044

Cited by 15 publications

(24 citation statements)

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“…6 for review). Applied to domestic species, flow cytometric techniques have been particularly straightforward in swine cytogenetic analyses, as most of the chromosomes in this species are both fairly different in size, and are fewer in number compared with human or bovine chromosomes (22).…”

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confidence: 99%

Swine chromosomal DNA quantification by bivariate flow karyotyping and karyotype interpretation

et al. 1992

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Human and swine chromosomes were analyzed separately and as a mix to obtain bivariate flow karyotypes. They were normalized to each other in order to use the human chromosomal DNA content as standard. Our results led to the characterization of the "DNA line" in swine identical to the human "DNA line." Estimation of the DNA content in megabase pairs of the swine chromosomes is proposed. Chromosomal assignment to the various resolved peaks on the bivariate swine flow karyotype is suggested from the relation between DNA content quantified by flow cytometry and chromosomal size. Swine chromosomes 1,13, 6,5,10,16,11,18, and Y were assigned to peaks A, B, C, K, L, N, 0, Q, and Y, respectively. Peaks D and E were assumed to contain chromosomes 2 and 14, but without specific assignment. Similarly, P and M peaks were expected to correspond to chromosomes 12 and 17. Of the remaining chromosomes (3, 7, X, 8, 15, 9, and 4), chromosomes 3, 7, and X, which were assigned previously to peaks F, G, and H, respectively, led us to deduce that chromosomes 15 and 8 belonged to peaks I and J, and chromosomes 9,4, and X to peak H. CI 1992 Wiley-Liss, Inc.Key terms: DNA line, chromosome identification Flow cytogenetic techniques already contribute greatly to our knowledge of the genome in mammals. They have, for instance, proved invaluable in detecting numeral and structural aberrations associated with human genetic diseases. They have also been extensively applied to human chromosome sorting, thereby permitting the construction of chromosome-specific DNA libraries (see ref. 6 for review). Applied to domestic species, flow cytometric techniques have been particularly straightforward in swine cytogenetic analyses, as most of the chromosomes in this species are both fairly different in size, and are fewer in number compared with human or bovine chromosomes (22).Flow cytogenetics and chromosome sorting therefore appear especially relevant for the ongoing European PiGMaP project (2). Among the aims of the project is the construction of chromosome-specific libraries, in order to provide as quickly as possible chromosome-assigned markers, which in conjunction with other techniques will allow coverage of the whole genome. A prerequisite to production of chromosome-specific libraries is accurate assignment of all, or at least most, of the swine chromosomes to the peaks of the flow karyotype and the estimation of the swine chromosomal DNA content. We therefore further developed studies on bivariate flow karyotypes in swine, as previous results showed that good chromosome resolution was easily achievable (7,s). All swine chromosome types could be resolved into individual peaks except two autosomal pairs plus chromosome X, which were confounded (15).The work reported here concerns the establishment of a "DNA line" in swine, a technique successfully applied to human chromosomes (17,3,18 somes to the various peaks on the bivariate flow karyotype. MATERIALS AND METHODSAnimals We analyzed the karyotypes of 11 pigs of both sexes, 9 males and 2 females...

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confidence: 99%

Swine chromosomal DNA quantification by bivariate flow karyotyping and karyotype interpretation

et al. 1992

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“…The data were processed by CorelDrdw version 3.0 and printed out. Landmarks and major bands were classified according to CSKSS (1988), and subband relationships were determined according to ISCN (1985) and kept as much as possible in agreement with the classification of YERLE et al (1991). The standard R-band idiogram (CSKSS 1988) also used in the figures was redrawn with CorelDraw version 3.0 and printed out.…”

Section: Methodsmentioning

confidence: 99%

“…The standard R-band idiogram (CSKSS 1988) also used in the figures was redrawn with CorelDraw version 3.0 and printed out. Idiograms from former classifications (LIN et al 1980;CSKSS 1988;RD"E 1990;YERLE et al 1991) were then compared with the new data, and differences between the classification systems listed ( Table 1). For direct comparison of corresponding idiograms, a scanning device and Scan man software were used in connection with CorelDraw version 3.0.…”

Section: Methodsmentioning

confidence: 99%

Localization of Landmarks and Bands in the Karyotype of Sus scrofa domestica. Comparison Between Different Classifications

Rønne

2004

Hereditas

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“…Physical and linkage map: Using the INRA somatic cell hybrid panel 4 and the IMpRH panel 5 , VCL has been assigned to Sscr14q2.5-q2.9 on the cytogenetic map and in 34 and 37 cR distance to microsatellites SW1536 and SW210 (LOD score 11.65 and 10.96) on the radiation hybrid (RH) map. The closest loci to VCL on the current public cytogenetic and RH maps are SW2612 and SW2593.…”

Section: Polymerase Chain Reaction (Pcr) Condition and Polymorphismmentioning

confidence: 99%

Erratum

2002

Animal Genetics

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The high-resolution GTG-banding pattern of pig chromosomes

Abstract: A high-resolution GTG-banded karyotype of the pig was obtained after methotrexate-induced cell synchronization. A diagrammatic representation of the banding pattern at the 539-band level is presented.

Cited by 15 publications

References 1 publication

Swine chromosomal DNA quantification by bivariate flow karyotyping and karyotype interpretation

Swine chromosomal DNA quantification by bivariate flow karyotyping and karyotype interpretation

Localization of Landmarks and Bands in the Karyotype of Sus scrofa domestica. Comparison Between Different Classifications

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