The hills and valleys of calcium signaling

Zhu, Michael X.; Tuo, Biguang; Yang, Jenny J.

doi:10.1007/s11427-016-5098-2

Cited by 7 publications

(3 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This acidic environment of lysosome not only is crucial to maintain the proton gradient, which affects the permeability of lysosomal membranes toward different ions, like Ca 2+ , but also regulates the activity of lysosomes, which influences the formation of autolysosomes ( Wan et al., 2014 ; Chung et al., 2019 ). Moreover, lysosome as significant “calcium pool” in live cells takes momentous roles in maintaining Ca 2+ homeostasis as well as signal transduction ( Zhu et al., 2016 ). For example, excessive reduction of Ca 2+ concentration in lysosome leads to lysosomal dysfunction, resulting in malfunction of cellular uptake and excretion, and further causes autophagy dysfunction and aging.…”

Section: Introductionmentioning

confidence: 99%

A DNA-Based FLIM Reporter for Simultaneous Quantification of Lysosomal pH and Ca2+ during Autophagy Regulation

2020

View full text Add to dashboard Cite

Summary pH and Ca 2+ play important roles in regulating lysosomal activity and lysosome-mediated physiological and pathological processes. However, effective methods for simultaneous determination of pH and Ca 2+ is the bottleneck. Herein, a single DNA-based FLIM reporter was developed for real-time imaging and simultaneous quantification of pH and Ca 2+ in lysosomes with high affinity, in which a specific probe for recognition of Ca 2+ was assembled onto a DNA nanostructure together with pH-responsive and lysosome-targeted molecules. The developed DNA reporter showed excellent biocompatibility and long-term stability up to ∼56 h in lysosomes. Using this powerful tool, it was discovered that pH was closely related to Ca 2+ concentration in lysosome, whereas autophagy can be regulated by lysosomal pH and Ca 2+ . Furthermore, Aβ-induced neuronal death resulted from autophagy abnormal through lysosomal pH and Ca 2+ changes. In addition, lysosomal pH and Ca 2+ were found to regulate the transformation of NSCs, resulting in Rapamycin-induced antiaging.

show abstract

Section: Introductionmentioning

confidence: 99%

A DNA-Based FLIM Reporter for Simultaneous Quantification of Lysosomal pH and Ca2+ during Autophagy Regulation

2020

View full text Add to dashboard Cite

show abstract

“…Since [Ca 2+ ] cyt rise may result from internal Ca 2+ release and/or extracellular Ca 2+ entry (Zhu MX et al, 2016), we further determined the Ca 2+ source of VIP-evoked [Ca 2+ ] cyt rise in GC cells.…”

Section: Ca 2+ Release In Gastric Cancer Cellsmentioning

confidence: 99%

VPAC1 couples with TRPV4 channel to promote calcium-dependent gastric cancer progression

Tang

Zhu³

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ca 2+ is a universal and versatile intracellular messenger regulating most biological processes, from gene expression to protein modification, and from cell proliferation to apoptosis [1][2][3][4]. Dysregulation of intracellular Ca 2+ underlies a variety of severe diseases, including cancer, neural degeneration and cardiovascular diseases [5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%