The histochemistry of carboxylester hydrolases: Problems and possibilities

Luppa, H; Andrä, Jörg

doi:10.1007/bf01042281

Cited by 12 publications

(8 citation statements)

References 118 publications

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“…11,21). The present study reports on the former and, thus, forms the basis of more detailed separation methods necessary for functional studies.…”

Section: Discussionmentioning

confidence: 93%

“…Inhibitors -The a-naphthyl acetate esterase enzymes comprise a variety of enzymes which may be cliaracterized by means of their sensitivity towards inhibitors and, to a certain degree, by their ability to hydrolyze specific artificial substrates (e.g. 11,21). The present study reports on the former and, thus, forms the basis of more detailed separation methods necessary for functional studies.…”

Section: Discussionmentioning

confidence: 94%

“…A-, B-and C-esterases(17,21) I A-and B-esterases(16)(17)(18) i A-and B-esterases(16)(17)(18) I A-, t C-, = B-esterase (II, 19)I A-and B-esterases (19) i some A-and B-esterase (20, 22-24) I choline esterases and some B-esterases, = most non-specific esterases (18, 25) I Epase, it = esterases (16, 24, 26) I esterases, = lipase (16, 24, 28) TOCP = triorthocresylphosphate; PCMB = parachloromercurybenzoate.…”

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confidence: 99%

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Presence of α‐naphthyl acetate esterase enzymes in dental and oral epithelium of guinea pig embryos

Juhl

Vilmann

1990

European J Oral Sciences

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Juhl M, Vilmann H: Presence of a-naphthyl acetate esterase enzymes in dentai and oral epithelium of guinea pig embryos. Scand J Dent Res 1990; 98: 286-94. Abstract ~ This study examines the distribution of a-naphthyl acetate esterase enzymes in reduced enamel epithelium, i.e., post secretory ameloblasts (PSA) and external cells of reduced enamel epithelium (ERE) of continuously growing incisor and molar tooth germs and in the covering oral epithelium (OE). Jaws of guinea pig embryos, 25-50 days of gestation age, were pretreated, frozen, serially cut, and incubated with a-naphthyl acetate as substrate and hexazotized pararosaniline as eapSure agent for demonstration of enzyme activity. In addition, sections were preincubated with vatious inhibitors of enzyme activity. A strong enzyme reaction, essentially unaffected by pretreatment procedures, was detnonstrated in all cells of PSA and ERE, and in suprabasal cells of OE. Preliminary characterization ofthe a-naphthyl acetate esterase enzymes by means of inhibitors suggests a prevailing presence of B-esterase enzymes in both oral and dental epithelia. PSA were selectively stained when 2x10"^ M arsanilic acid was used as inhibitor, ERE were selectively inhibited by 10'^ M eserine, and cells of OE were selectively stained when 2 x 10"^ M HgClj was used as inhibitor. We therefore conclude that the a-naphthyl acetate esterase enzymes in combination with inhibitors may serve as histochemieal markers for discrimination of dental and oral epithelium in the guinea pig prior to eruption of teeth.

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“…11,21). The present study reports on the former and, thus, forms the basis of more detailed separation methods necessary for functional studies.…”

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

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Presence of α‐naphthyl acetate esterase enzymes in dental and oral epithelium of guinea pig embryos

Juhl

Vilmann

1990

European J Oral Sciences

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“…On the basis of their behaviour towards substrates, inhibitors and activators, three main groups are distinguished, i.e. A, B and C esterases (Luppa & Andra, 1983). B-type esterases especially have a high affinity for ANB.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural cytochemistry of non-specific esterase in murine peritoneal macrophages

et al. 1989

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Non-specific esterase (NSE) activity was demonstrated in glutaraldehyde-fixed monolayers of murine peritoneal macrophages. Using 2-naphthylthiol acetate (NTA) as substrate and Fast Blue BB as coupling agent a strong osmiophilic reaction product was obtained. The reaction product was observed as electron-dense dots covering cisterns of the rough endoplasmic reticulum, Golgi saccules and vesicles, or as large aggregates in lysosomes. Using alpha-naphthyl butyrate (ANB) as substrate and hexazotized pararosaniline as coupling agent the osmiophilic reaction product was observed extracellularly on the plasma membrane as an electron-dense continuous layer, whereas intracytoplasmic staining of lysosomes was rare. Substitution of the coupling agents in the respective media resulted in a slight reaction with the ANB medium whereas with the NTA medium reaction product was observed only in lysosomal structures. The substrate specificity of the different types of esterases was confirmed after isoelectric focusing on thin-layer polyacrylamide gels. The results indicate that in murine peritoneal macrophages different types on NSE are detected with NTA and ANB, having distinct ultrastructural localizations.

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“…" "^K Biologic functions of nonspecific esterases are at present obscure and speculative, and their natural substrates are mostly unknown (1). Despite this, esterases seem to be suitable markers for various cells and tissues during development and differentiation (2,3).…”

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confidence: 99%

Epithelial α‐naphthyl acetate esterases in the green vervet monkey gingiva before and after periodontal surgery and during tooth eruption

Juhl

Holmstrup

1993

European J Oral Sciences

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To provide enzymatic information on de novo formed junctional (JE) and sulcular epithelium (SE), we performed periodontal surgery on 24 teeth. Ten to 14 days postoperatively, all experimental and 16 control teeth were extracted with adjacent buccal gingiva. In addition, specimens from unerupted and partly erupted teeth containing enamel epithelium (EE) were examined. Fixed cryostat sections were cut in series, stained with HE, or incubated with and without substrate for demonstration of α‐naphthyl acetate esterase activity and for control purposes, respectively. The distribution and intensity of the α‐naphthyl acetate esterase activity of newly reformed JE and SE was identical to that of the original JE and SE, i.e. suprabasal and very strong. In contrast, both the oral gingival epithelium (OGE) and the EE displayed a very weak enzyme reaction. These observations indicate that the presence of α‐naphthyl acetate activity of original and reformed JE and SE is probably site specific and of nondevelopmental origin. Heavy inflammation after healing was associated with enhanced epithelial proliferation of OGE and, in addition, marked esterase activity of these proliferations and corresponding OGE. This points at a possible inflammatory induction of the marked esterase activity seen in JE and SE as well as site‐specific, connective tissue influences. Further investigation is needed to elucidate the effect of inflammation on the esterase activity.

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The histochemistry of carboxylester hydrolases: Problems and possibilities

Cited by 12 publications

References 118 publications

Presence of α‐naphthyl acetate esterase enzymes in dental and oral epithelium of guinea pig embryos

Presence of α‐naphthyl acetate esterase enzymes in dental and oral epithelium of guinea pig embryos

Ultrastructural cytochemistry of non-specific esterase in murine peritoneal macrophages

Epithelial α‐naphthyl acetate esterases in the green vervet monkey gingiva before and after periodontal surgery and during tooth eruption

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