The Histochemistry of Sialic Acid Containing Mucoproteins

Spicer, S. S.; Warren, Leonard

doi:10.1177/8.2.135

Cited by 254 publications

(41 citation statements)

References 2 publications

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“…Lability of PAS staining to malt diastase identified glycogen (93). Elimination of basophilia by sialidase localized glycoprotein possessing sialic acid residues (216). Removal of basophilia by digestion with bacterial hyaluronidase (94) localized hyaluronic acid.…”

Section: (205)mentioning

confidence: 99%

Diversity of cell glycoconjugates shown histochemically: a perspective.

Spicer

Schulte²

1992

J Histochem Cytochem.

380

297

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IntroductionThe earliest histochemical methods for demonstrating carbohydratecontaining macromolecules served mainly to &rentiate these substances, as a general class, from the other macromolecular constituents in tissue sections: the proteins, nucleic acids, and lipids (93,137). A later stage in the development of this field aimed at distinguishing by histochemistry subclasses of carbohydratecontaining components, e.g., neutral and acidic constituents and subgroups of the acidic substances. The capacity to characterize complex carbohydrates in situ in tissue sections progressed subsequently with the use of carbohydrate-degrading enzymes to locate moieties possessing a specific carbohydrate linkage labile to the enzyme and of immunostaining to identlfy specific epitopes. An added dimension for analysis of glycoconjugate (GC) structure in situ followed the advent of labeled lectins which allow histochemical demonstration of terminal or internal sugars or of sugar sequences.This review attempts to put in perspective the status of carbohydrate histochemistry before and after introduction of the lectin approach. Progress in the development and application of non-lectin methods for light and electron microscopic localization has been reviewed (126,137,153,172,175,205,210,213,234) and will be covered here in summary form. A voluminous literature on lectin histochemistry and its use in a wide range of adult and embryonic tissues and pathological lesions at the light and electron microscopic level has also been reviewed extensively tinctive chemical structure in diverse sites of highly specialized biologica1 activity. Such an association implies relevance of the chemical nature of the GC to the function where it occurs. A number of examples of such concurrence will be described that point to possible participation of a specific GC in a particular biological function at a precise histological or cytological site. Pre-lectin Histochemistry of Complex CarbohydratesA large class of macromolecules in mammalian tissues differs from the other classes in possessing sugar residues. These carbohydratecontaining components are referred to in general as complex carbohydrates, a term that includes both polysaccharides, such as glycogen, and glycoconjugates (GCs), in which sugars are linked to non-carbohydrate moieties. Histochemical methods specific for visualizing GCs and differentiating them from proteins, nucleic acids, and lipids depended initially on their content of sugars with vicinal hydroxyls and of distinctive acidic moieties (26) (Figure 1; Eble 1). Thus, GCs which alone among the classes of macromolecules contain hexoses with vicinal hydroxyls were visualized selectively with the periodic acid-Schiff (PAS) procedure (93, 109). In addition, highly acidic sulfate esters and less acidic carboxyls of sialic acids and uronic acids in G C s were found to bind a variety of cationic dyes that did not react with less acidic dicarboxylic amino acids in proteins or with nucleic acids. Basic reagents commonly used included alcian bl...

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Section: (205)mentioning

confidence: 99%

Diversity of cell glycoconjugates shown histochemically: a perspective.

Spicer

Schulte²

1992

J Histochem Cytochem.

380

297

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“…According to SPICER and WARREN [ 6 ] , acetylneuraminic (sialic) acid is indicated by the disappearance of AB staining with preservation of PAS activity after incubation in a buffered solution of neuraminidase.…”

Section: Methodsmentioning

confidence: 99%

Mucinous Degeneration of the Epithelium of the Urinary Tract of Swine

1971

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Abstract. Mucinous degenerative change was observed in the epithelial cells lining the renal pelvis, ureter, and urinary bladder of pigs with exudative epidermitis, coliform enteritis, hog cholera, and suppurative arthritis. Mucins were observed within transitional cells either as granular or homogenous material within vacuoles. Lakes filled with mucins also were formed as a result of the coalescence of mucin from degenerating transitional cells. The cells and lakes of mucin were stained selectively by periodic acid-Schiff, alcian blue, and colloidal iron. On the basis of the reactivity patterns with these stains the transitional epithelial cells were considered capable of producing acidic and neutral mucins.The potential of the urinary tract epithelium for secretion of mucus has been observed in many species of animals. In chronic proliferative cystitis of cattle, the transitional epithelium may undergo metaplasia to mucus-secreting goblet-shaped cells arranged in glandular patterns [I]. In man, mucus-secreting epithelial metaplasia has been observed in the renal pelvis, ureter, and urinary bladder [2,5]. A mucinous degeneration has been observed in the epithelium of the renal pelvis and ureter of pigs with exudative epidermitis [3]. In these pigs, dilatation of the ureters and renal pelves resulted from ureteral obstruction caused by edema, inflammatory cellular infiltrate, hyperplasia, and mucinous degeneration of the epithelium.Mucinous degeneration of the epithelium of the urinary tract of swine with diseases other than exudative epidermitis and the histochemical nature of the mucinous material are reported. Materials and MethodsThe mucosa of the renal pelvis, ureter, and urinary bladder of 49 diseased pigs and eight healthy pigs was studied. Twenty-one of the pigs, varying in age from 1-6 weeks, had

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“…Azul alcian (AB) a pH 0,5 (Lev & Spicer, 1964), AB a pH 2,5 (Mowry & Winkler, 1956), AB (pH 2,5) después de metilación, AB (pH 2,5) después de metilación + saponificación (Spicer & Lillie, 1959), AB (pH 2,5) después de digestión con neuraminidasa (Spicer & Warren, 1960), AB (pH 2,5) después de hidrólisis ácida (Quintarelli et al, 1961), AB (pH 2,5) después de digestión con hialuronidasa testicular (Quintarelli, 1963) y técnica combinada PAS + AB (pH 2,5) (Mowry & Winkler). Proteínas.…”

Section: Materials Y Métodounclassified

Estudio Morfológico e Histoquímico de las Glándulas Cefálicas de Bothrops alternatus (Ophidia, Viperidae)

Parpinelli¹,

Lopes²,

Sala³

et al. 2013

Int. J. Morphol.

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RESUMEN:El objetivo del presente trabajo fue estudiar la histología de las glándulas cefálicas de la víbora de la cruz (Bothrops alternatus), identificar los tipos celulares y caracterizar histoquimicamente el producto de secreción. Con esta finalidad fueron usados ejemplares machos adultos de B. alternatus. Después del sacrificio, las glándulas cefálicas fueron fijadas en formol neutro al 10% e incluidas en parafina. Los cortes, de 6 µm de espesor, fueron teñidos con hematoxilina y eosina, tricrómico de Masson y con técnicas histoquímicas para identificación de mucopolisacáridos y proteínas. Los resultados histológicos demostraron que las glándulas linguales, de veneno y accesorias son de tipo tubular, las glándulas labiales y premaxilar son tubuloacinares y la glándula de Harder es tubuloacinar compuesta. Las células mucosas de los adenómeros y conductos secretores de las glándulas linguales, labiales y premaxilar, así como las células de los conductos de la glándula de Harder, secretan sulfosialomucinas y mucopolisacáridos neutros. Las células mucoserosas de las glándulas linguales, labiales y premaxilar sintetizan mucopolisacáridos neutros, ácido siálico y radicales tirosina, cistina, cisteína, arginina y triptófano. Las células seromucosas de las glándulas de veneno, accesorias y de Harder producen polisacáridos neutros y radicales cistina, cisteína y triptófano, mientras que la glándula de Harder también sintetiza tirosina.

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The Histochemistry of Sialic Acid Containing Mucoproteins

Cited by 254 publications

References 2 publications

Diversity of cell glycoconjugates shown histochemically: a perspective.

Diversity of cell glycoconjugates shown histochemically: a perspective.

Mucinous Degeneration of the Epithelium of the Urinary Tract of Swine

Estudio Morfológico e Histoquímico de las Glándulas Cefálicas de Bothrops alternatus (Ophidia, Viperidae)

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