The Histochemistry of Simple Esterase

Malaty, H. A.; Bourne, G. H.

doi:10.1159/000141005

Cited by 12 publications

(5 citation statements)

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“…Among these are the following: for PMN, (references 58, 77-81), for MN (58,77,(80)(81)(82)(83)(84)(85)(86)(87)(88)(89)(90)(91), and for AM (82,87,89,90,92). With few exceptions, the inhibitor studies reported in the literature (77,(82)(83)(84)(85)(86)(87)(88)92) agree with our results. AM and MN appear to contain both the inhibitor-sensitive and inhibitor-resistant varieties of esterase described by Shnitka and Seligman (93).…”

Section: Survey Of Literaturesupporting

confidence: 91%

“…There are many reports that describe esterases in tissue phagocytes. Among these are the following: for PMN, (references 58, 77-81), for MN (58,77,(80)(81)(82)(83)(84)(85)(86)(87)(88)(89)(90)(91), and for AM (82,87,89,90,92). With few exceptions, the inhibitor studies reported in the literature (77,(82)(83)(84)(85)(86)(87)(88)92) agree with our results.…”

Section: Survey Of Literaturesupporting

confidence: 91%

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A Histochemical Study of Phagocytic and Enzymatic Functions of Rabbit Mononuclear and Polymorphonuclear Exudate Cells and Alveolar Macrophages

Dannenberg

Burstone

Walter

et al. 1963

The Journal of Cell Biology

127

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The cytochromc oxidasc (CO), aminopcptidasc (AMP), succinic dchydrogcnasc (SD), acid phosphatasc, cstcrasc, and alkaline phosphatasc of rabbit mononuclcar (MN) and polymorphonuclcar (PMN) peritoneal cxudatc cells and pulmonary alveolar macrophagcs (AM) -air dried on Mylar strips-were characterized by histochcmical techniques with respect to stability, activators, inhibitors, and pH optima. A granule count method was established for the quantitation of these cnzymcs. For the acid phosphatasc of MN, in which the most precise results wcrc obtained, time, pH, substratc, and inhibitor curves resembled those commonly obtained biochcmically. Five of these enzymes wcrc usually more active in AM than MN, whereas the sixth, alkaline phosphatasc, was not present in cithcr cell type. AM also tended to consume more oxygen than MN and to divide more frequently. Since the most active cells in thc population would bc first involved in the host's defense against microbial agents, a comparison was made of the 10 per cent of the AM and MN with the highest enzymatic activities. No differences wcrc found in the granule counts that wcrc not reflected by the means. However, within a given AM population, cells containing ingested dust particles sccmcd to have higher enzymatic activities than those without particles. MN had greater acid phosphatasc and SD activities than PMN and consumcd more oxygen, but the CO, AMP, and cstcrasc activitcs of both types of cells wcrc of similar magnitude. PMN showed high alkaline phosphatasc activity; MN showed none. A survcy of the histochcmical litcraturc indicates that a positive correlation between the enzymatic and phagocytic activities of both MN and PMN exists in vivo.Mononuclear phagocytes (MN) 1 are essential components of the reticuloendothelial system--the chief defense of the host against many micro organisms and their products. Similar functions i The abbreviations used in this report are as follows : MN, mononuclear(s), or mononuclear peritoneal exudate cells; PMN, polymorphonuclear(s) or polymorphonuclear peritoneal exudate cells; AM, are served by polymorphonuclears (PMN) in the blood and in areas of inflammation, and by alveolar macrophages (AM) in the lung. The present report (a) characterizes six enzymes of rabbit MN alveolar macrophage(s) of the lung; ip, intraperitoneal; CO, cytochrome oxidase; AMP, aminopeptidase; SD,succinic dehydrogenase; Tris, tris(hydroxymethyl) aminomethane. 465on

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Section: Survey Of Literaturesupporting

confidence: 91%

Section: Survey Of Literaturesupporting

confidence: 91%

A Histochemical Study of Phagocytic and Enzymatic Functions of Rabbit Mononuclear and Polymorphonuclear Exudate Cells and Alveolar Macrophages

Dannenberg

Burstone

Walter

et al. 1963

The Journal of Cell Biology

127

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“…On the basis of our experiments on the pharmacokinetics (29, 30) and on the specificity in vivo (31) of this inhibitor the application of 3H-BNPP for quantitative ultrastructural localization of CE has turned out to be evidently advantageous and reasonable, since it allows quantitative labelling in vivo (29,37,40) and avoids the use of protective substrates. (14,15,26) and other organs of the male genitals (13,21,25) show high levels of esterase activities -the ranking depends on the substrate chosen for the enzymatic assay (37). In 1961 SHNITKA and SELIGMAN (18) reviewed the affords by that time to discriminate histochemically the unspecific carboxylesterases (which are classified as B-type-esterases on account of their susceptibility to organophosphates (ALDRIDGE, 38) from other esterases by differential inhibition.…”

Section: Discussionmentioning

confidence: 99%

Autoradiographic study on the distribution of Bis-p-nitrophenylphosphate (BNPP), a specific inhibitor of carboxylesterases, in the rat

Block

Wassermann

1978

European Journal of Drug Metabolism and Pharmacokinetics

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“…Nachlas and Seligman (1949b) reported an absence of esterase activities in the cytoplasm of epithelial cells in the human jejunum. On the other hand, the nonspecific esterase activity has been claimed to be almost absent, by light microscopy, from the striated border and to be intense at the supranuclear cytoplasm of the intestinal epithelial cell in the rat duodenum and jejunum, by Malaty and Bourne (1955) Shnitka (1960) maintained that the supranuclear cytoplasmic region with intense esterase activities corresponds to the Golgi area of the epithelial cell. In the present study, however, electron microscopy did not reveal the Golgi apparatus with reactions for esterases.…”

Section: Discussionmentioning

confidence: 99%

“…Since then, contributions have been made in the field of nonspecific esterase histochemistry for the development of azo dye technique by Nachlas and Seligman (1949a, b), Gomori (1952), Chessick (1953), Malaty and Bourne (1955), Shnitka (1960), Padykula, Strauss, Ladman and Gardner (1961), Abe, Kramer and Seligman (1964), Friedman, Strachan and Dewey (1966), and Geyer and Nietzold (1968). Barrnett (1952a, b) introduced indoxyl acetate as a substrate for the enzyme reaction.…”

mentioning

confidence: 99%

Electron Microscopic Demonstration of Nonspecific Esterases in the Small Intestine of the Rat

Yokota

1974

Acta Histochem. Cytochem

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The nonspecific esterase activity was investigated at the electron microscopic level, by means of azo dye technique applied to the duodenal, jejunal and ileal mucosae of living rats. After perfusion of a medium containing 1-naphthyl acetate and hexazonium pararosaniline into the lumen of the small intestine, the enzyme reaction product of electron opaque grains (50-100 nm in diameter) was localized on the limiting membrane of microvilli of epithelial cells, and on the vesicles and the granular endoplasmic reticulum in the apical cytoplasm of epithelial cells. When the medium was perfused intravascularly, electron opaque grains were localized on the basal cell membrane and the basal lamina of epithelial cells as well as on the vesicles present in the basal cytoplasm of epithelial cells. Localizations of the esterase activity were essentially alike whether naphthyl propionate or butyrate was used as a substrate.The esterase activity in the duodenal and jejunal mucosae was higher than that in the ileal mucosa, judging from the results of inhibition test and reactivation test.In earlier histochemical works using the light microscope, Gomori (1945Gomori ( , 1948 reported occurrence in the mucosa of rat small intestine of the nonspecific esterases as demonstrated by means of Tween's method. Since then, contributions have been made in the field of nonspecific esterase histochemistry for the development of azo dye technique by

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The Histochemistry of Simple Esterase

Cited by 12 publications

References 1 publication

A Histochemical Study of Phagocytic and Enzymatic Functions of Rabbit Mononuclear and Polymorphonuclear Exudate Cells and Alveolar Macrophages

A Histochemical Study of Phagocytic and Enzymatic Functions of Rabbit Mononuclear and Polymorphonuclear Exudate Cells and Alveolar Macrophages

Autoradiographic study on the distribution of Bis-p-nitrophenylphosphate (BNPP), a specific inhibitor of carboxylesterases, in the rat

Electron Microscopic Demonstration of Nonspecific Esterases in the Small Intestine of the Rat

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