The Histological Characteristics and Localisation of ACP and ANAE Positive Lymphocytes in the Oesophageal Tonsil of the Duck (Anas Platyrhynchos)

Dönmez, Hasan Hüseyin; Eken, Emrullah; Beşoluk, Kamil; Sur, Emrah

doi:10.3184/175815512x13264771062961

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…Then, cryostat sections (12 μm) were taken from samples. These preparations were stained for ANAe and ACP-ase demonstrations [19] . Both PB and uterine tissue samples were stained with 1% methyl green (Merck) for counterstain.…”

Section: Collection and Processing Of Tissue Samplesmentioning

confidence: 99%

Deneysel Hipotiroidizm Oluşturulan Ratlardaki Periferik Kan ve Endometriyum Dokularında ANAE ve ACP-az Pozitif Lenfositlerin Belirlenmesi

Çolakoğlu¹,

Dönmez²

2018

Kafkas Univ Vet Fak Derg

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Section: Collection and Processing Of Tissue Samplesmentioning

confidence: 99%

Deneysel Hipotiroidizm Oluşturulan Ratlardaki Periferik Kan ve Endometriyum Dokularında ANAE ve ACP-az Pozitif Lenfositlerin Belirlenmesi

Çolakoğlu¹,

Dönmez²

2018

Kafkas Univ Vet Fak Derg

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“…İnkübasyon solüsyonları içerisinde oda sıcaklığında 15 dak. süreyle kontrollü bir şekilde bekletilen kesitlerdeki lenfositlerde kırmızı-kahverengi granüllerin ortaya çıkmasının ardından %1'lik methylgreen ile çekir-dek boyası uygulandı [15] .…”

Section: Metotunclassified

The Proportion and The Distribution of T-lymphocytes, Null Lymphocytes and Acid Phosphatase Positive Lymphocytes of The Peripheral Blood and Endometrium in Pregnant Mice

Sur¹,

Öznurlu²,

Özaydin³

et al. 2014

Kafkas Univ Vet Fak Derg

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Bu çalışma, farelerde gebeliğin T-lenfosit, null lenfosit ve asit fosfataz (ACP-az) pozitif lenfositlerin perifer kandaki oranı ve endometriyumun desidua bazalis bölgesindeki dağılımı üzerindeki etkilerinin belirlenmesi amacıyla yapıldı. Bu amaçla, 12-14 haftalık fareler, gebe olmayan-kontrol grubu ile gebeliğin birinci, ikinci ve üçüncü haftalarının ortasına karşılık gelecek şekilde erken, orta ve geç gebelik dönemi (sırasıyla gebeliğin 3., 10. ve 17. günleri) olmak üzere 4 gruba ayrıldılar (n = 6). En düşük T-lenfosit oranı perifer kanda (%43.83) ve desidua bazalis dokusunda (10.83 adet/0.1 mm 2 ) sırasıyla gebeliğin erken ve orta dönemlerinde tespit edildi. Perifer kan ACP-az pozitif lenfosit oranlarında gebeliğin ikinci haftasında istatistiksel olarak önemli bir yükselme gözlenirken (%44.33); desidua bazalis dokusunda en düşük ACP-az pozitif lenfosit sayısı (5.50 adet/0.1 mm 2 ) gebeliğin erken döneminde gözlendi. Hem perifer kanda (%11.50) ve hem de desidua bazalis dokusunda (7.83 adet/0.1 mm 2 ) en yüksek null hücre oranı erken gebelik döneminde tespit edildi. En düşük perifer kan lenfosit oranı (%56.00) erken dönemde gözlendi. Dönemler arasında bazı farklar olsa da gebeliğin lenfosit, T-lenfosit, null lenfosit ve ACP-az pozitif lenfositlerinin sayı ve dağılımlarını etkilediği sonucuna varıldı. Anahtar sözcükler: ACP-az, ANAE, Fare, Gebelik The Proportion and The Distribution of T-lymphocytes, Null Lymphocytes and Acid Phosphatase Positive Lymphocytes of The Peripheral Blood and Endometrium in Pregnant Mice SummaryThis study was performed to determine the effects of pregnancy on the proportion of T-lymphocytes, null lymphocytes and acid phosphatase (ACP-ase) positive peripheral blood lymphocytes and the distribution of the mentioned cells in the decidua basalis region of endometrium in the pregnant mice. For this purpose, mice at 12-14 weeks of age were divided into four groups as non-pregnant control, and at the middle of the first, second, and the third week of the pregnancy, corresponding to early, middle, and late (3 rd , 10 th , and 17 th days of pregnancy) gestational stages respectively (n = 6 for each group). The lowest T-lymphocytes percentage was determined at early and middle pregnancy in the peripheral blood (43.83%) and decidua basalis (10.83 number/0.1 mm 2 ), respectively. There was a statistically significant increase in the proportions of the peripheral blood ACP-ase (+) lymphocytes (44.33%) at the mid-gestational period while the lowest ACP-ase positive lymphocyte numbers (5.50 number/0.1 mm 2 ) in the decidua basalis was observed at early pregnancy. The highest null cell rates were found at early gestation either in the peripheral blood (11.50%) or in the decidua basalis (7.83 umber/0.1 mm 2 ). The lowest percentage of peripheral blood lymphocyte (56.00%) was recorded at the early pregnancy. It was concluded that the number and the distribution of the lymphocyte, T-lymphocyte, null lymphocyte, and ACP-ase positive lymphocyte were affected by pregnancy although there were some differences...

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“…Plasma cells, which are effector cells of Blymphocytes, produce immunoglobulins from which antibodies are constructed. Antibodies are able to bind and neutralize antigens that have entered the body (Donmez et al, 2012;Oláh et al, 2013;Logvinova et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Content and location of lymphocyte subpopulations with markers CD4+, CD8+ and CD20+ in the esophageal tonsil of chickens and the Meckel diverticulum of ducks

Хомич

Дышлюк

Mazurkevych

et al. 2021

Regul. Mech. Biosyst.

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Immune formations of birds' digestive organs, including the esophageal tonsil and Meckel’s diverticulum, protect the body from foreign antigens that enter the body with food and water and play an important role in maintaining the genetic constancy of its internal environment. This unique property of the immune system is formed during ontogenesis and is associated with maintaining the selection of lymphocyte clones that are able to respond to foreign antigens and carry out a specific immune response of two types: humoral and cellular. This article presents the results of a study of T- and B-lymphocyte subpopulations of the esophageal tonsil of Shever 579 cross chickens at the age of 25, 180 and 300 days, the Meckel diverticulum of the Blagovarsky cross ducks at the age of 30, 150 and 180 days. Immunohistochemical and statistical research methods were used to determine the localization and quantitative parameters of cell populations of lymphocytes (CD4+, CD8+, CD20+) using monoclonal antibodies and the DAKO EnVision FLEX+ imaging system (Dako Cytomation, Denmark). Separate subpopulations of T-lymphocytes (CD4+ - helpers, CD8+ -cytotoxic / T-suppressors) and mature B-lymphocytes (CD20+) were found in the esophageal tonsil and Meckel diverticulum of birds. Their presence confirms that antigen-independent proliferation and differentiation of lymphocytes into effector cells occur in the immune formations of the digestive system. The lymphoid tissue of these formations is represented mainly by a well-defined diffuse form and nodules with light centers (secondary). In the esophageal tonsil of chickens, these structures are located in the tunica mucosa and tela submucosa, and in the Meckel diverticulum of ducks – also in the tunica muscularis. The content of lymphocytes with these markers predominates in diffuse lymphoid tissue compared to that in secondary lymphoid nodules. In the diffuse lymphoid tissue of the esophageal tonsil, lymphocytes are located mainly near the adenomeres and excretory ducts of the esophageal glands, blood vessels, and under the surface epithelium, and in Meckel’s diverticulum – around the crypts, in their epithelium and in the epithelium of the villi. They are also found in the light centers of lymphoid nodules and on their periphery. The indices of the content of lymphocytes with the indicated markers in the esophageal tonsil and Meckel diverticulum which we determined were associated with age characteristics of the poultry in the postnatal period of ontogenesis. According to our observations, the content of CD20+ lymphocytes was the highest, while the populations of CD4+ and CD8+ lymphocytes were much smaller. This indicates an increase in the activity and predominance of the humoral immunity over the cellular one. The content of CD20+ lymphocytes was highest in birds at the age of 180 days, that is, during their sexual maturity. The data presented in the work can be used by morphologists researching the organs of the immune system, immunologists, poultry specialists involved in breeding, using and raising poultry and in educational work.

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The Histological Characteristics and Localisation of ACP and ANAE Positive Lymphocytes in the Oesophageal Tonsil of the Duck (Anas Platyrhynchos)

Cited by 5 publications

References 14 publications

Deneysel Hipotiroidizm Oluşturulan Ratlardaki Periferik Kan ve Endometriyum Dokularında ANAE ve ACP-az Pozitif Lenfositlerin Belirlenmesi

Deneysel Hipotiroidizm Oluşturulan Ratlardaki Periferik Kan ve Endometriyum Dokularında ANAE ve ACP-az Pozitif Lenfositlerin Belirlenmesi

The Proportion and The Distribution of T-lymphocytes, Null Lymphocytes and Acid Phosphatase Positive Lymphocytes of The Peripheral Blood and Endometrium in Pregnant Mice

Content and location of lymphocyte subpopulations with markers CD4+, CD8+ and CD20+ in the esophageal tonsil of chickens and the Meckel diverticulum of ducks

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