“…High throughput expression analyses have been used to identify characteristics of embryogenic explants (Salvo, Hirsch, Buell, Kaeppler, & Kaeppler, 2014; Trontin, Klimaszewska, Morel, Hargreaves, & Lelu‐Walter, 2016; Wickramasuriya & Dunwell, 2015; Yang et al., 2012), but most studies use whole explants, which contain a complex mixture of tissues and cell types, making it difficult to specifically assign molecular identities to embryogenic cells. In situ gene expression analyses would help to resolve the sometimes contradictory relationship between expression of developmental marker genes and cell fate in different culture systems (Miguel & Marum, 2011) and provide a more exact description of when and how embryogenic cells are formed in culture (Li et al., 2014; Soriano et al., 2014). …”