2014
DOI: 10.1105/tpc.113.116491
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The Histone Deacetylase Inhibitor Trichostatin A Promotes Totipotency in the Male Gametophyte

Abstract: The haploid male gametophyte, the pollen grain, is a terminally differentiated structure whose function ends at fertilization. Plant breeding and propagation widely use haploid embryo production from in vitro-cultured male gametophytes, but this technique remains poorly understood at the mechanistic level. Here, we show that histone deacetylases (HDACs) regulate the switch to haploid embryogenesis. Blocking HDAC activity with trichostatin A (TSA) in cultured male gametophytes of Brassica napus leads to a large… Show more

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Cited by 117 publications
(189 citation statements)
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References 103 publications
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“…This result in Arabidopsis suggests that axillary bud initiation requires a permissive configuration of the chromatin so that WUS can be induced by a cytokinin pulse (Wang et al 2017). In line with this hypothesis, it was shown previously that the induction of somatic embryogenesis by auxin, which also involves de novo meristem formation, can be significantly improved by TSA treatment (Li et al 2014a). In order to predict whether similar mechanisms play a role in tulip, a deeper understanding of the initiation of tulip axillary meristem initiation is required.…”
Section: What Can We Learn From Axillary Bud Outgrowth In Tulip?supporting
confidence: 68%
“…This result in Arabidopsis suggests that axillary bud initiation requires a permissive configuration of the chromatin so that WUS can be induced by a cytokinin pulse (Wang et al 2017). In line with this hypothesis, it was shown previously that the induction of somatic embryogenesis by auxin, which also involves de novo meristem formation, can be significantly improved by TSA treatment (Li et al 2014a). In order to predict whether similar mechanisms play a role in tulip, a deeper understanding of the initiation of tulip axillary meristem initiation is required.…”
Section: What Can We Learn From Axillary Bud Outgrowth In Tulip?supporting
confidence: 68%
“…High throughput expression analyses have been used to identify characteristics of embryogenic explants (Salvo, Hirsch, Buell, Kaeppler, & Kaeppler, 2014; Trontin, Klimaszewska, Morel, Hargreaves, & Lelu‐Walter, 2016; Wickramasuriya & Dunwell, 2015; Yang et al., 2012), but most studies use whole explants, which contain a complex mixture of tissues and cell types, making it difficult to specifically assign molecular identities to embryogenic cells. In situ gene expression analyses would help to resolve the sometimes contradictory relationship between expression of developmental marker genes and cell fate in different culture systems (Miguel & Marum, 2011) and provide a more exact description of when and how embryogenic cells are formed in culture (Li et al., 2014; Soriano et al., 2014). …”
Section: Definitions and Conceptsmentioning
confidence: 99%
“…Germinating spruce somatic embryos treated with TSA maintain their embryo identity rather than converting to seedlings (Uddenberg et al., 2011). TSA was also found to enhance haploid embryo induction from heat‐stressed in vitro cultured male gametophytes in B. napus (Li et al., 2014). These data indicate that histone deacetylation is a conserved mechanism in plants to repress embryo identity outside the seed.…”
Section: The Role Of Chromatin Modifications In Somatic Embryogenesismentioning
confidence: 99%
“…The most widely used method involves in vitro regeneration of plants from haploid cells or tissues, a procedure both tedious and expensive. Arabidopsis thaliana and many crop species have proven to be recalcitrant to in vitro haploid production 5,6 . In some species, wide crosses [7][8][9][10][11][12] or intraspecific crosses to lines with specific genetic determinants 13,14 result in in vivo haploid embryo because the chromosomes of the haploid inducer (HI) are lost during postzygotic mitotic divisions while the chromosomes of the non-inducer parent are retained 15 .…”
mentioning
confidence: 99%