The histopathological comparison on the destruction of the periodontal tissue between normal junctional epithelium and long junctional epithelium

Noguchi, Satoshi; Ukai, Takashi; Kuramoto, Andréia; Yoshinaga, Y.; Nakamura, H.; Takamori, Yuzo; Yamashita, Yasunori; Hara, Yoshitaka

doi:10.1111/jre.12370

Cited by 26 publications

(32 citation statements)

References 32 publications

(36 reference statements)

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“…The thickness of JE was measured by counting the number of epithel layers from the cemento-enamel junction (CEJ) to the most coronal and apical sections of JE. 22 The PAS positive gland staining produced a magenta-purple colour in the duct cell. The mucin level was measured based on the density of the mucin cell expression in the submandibular gland.…”

Section: Methodsmentioning

confidence: 99%

Differences in mucin expression in the submandibular glands of rats during peridontitis induction

2018

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Background: Porphyromonas gingivalis (Pg) produces lipopolysacharide (LPS) which acts as a stimulator of inflammation in periodontal tissues. Periodontitis-induced apoptosis and vacuolation of the salivary gland, therefore, causes hyposalivation. Mucin secretion is produced by the submandibular gland under stimulation by the cholinergic and adrenergic receptors. Both forms of stimulation influence the volume of mucin secretion. Mucin saliva plays an important role in the early stages of Pg colonization in the oral cavity. On the other hand, it serves to protect against bacterial invasion. Purpose: The aim of this research was to identify differences in mucin expression in the submandibular gland during periodontitis induction. Methods: 32 male Wistar rats were assigned to either a sham periodontitis or a periodontitis group. The former group received a daily injection of a vehicle solution (n = 16), while members of the periodontitis induction group (n=16) were injected each day with 500 µL of Pg 108 into the mesial area of the upper molar. Mucin in the submandibular gland was analyzed at the 7th, 14th, 21th and 28th days after injection by means of periodic acid schiff (PAS) staining. Results: 28 days after injection mild gingivitis was developed in the periodontitis experiment group. Junctional epithelium (JE) thickness decreased gradually following the increase of PG injection periods (p<0.05). However, mucin expression increased prominently at 7th, 14th, and 21th days after injection and decreased on day 28th after PG injection. Mucin was expressed in the duct cells of the submandibular gland. Conclusion: The result of this study suggests that there are different levels of mucin expression in the submandibular gland during periodontitis induction.

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Section: Methodsmentioning

confidence: 99%

Differences in mucin expression in the submandibular glands of rats during peridontitis induction

2018

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“…Blood samples (150 μL) from the retro‐orbital venous plexus of rats under general anesthesia with isoflurane were collected into microhematocrit tubes just before the first topical application (baseline) and immediately after the 5th and 10th applications. The levels of anti‐LPS IgG in serum samples of individual rats were determined by an indirect ELISA, as described previously . Ninety‐six‐well microtiter plates were coated (100 μL/well) with a 2.5 μg/mL solution of LPS from E. coli in 0.05 M carbonate buffer (pH 9.6) and incubated for 16 hours at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…Immunohistological staining of complement component C1q was performed to detect immune complexes, as described previously . After the sections were deparaffinized, endogenous peroxidase activity was blocked with 0.3% H 2 O 2 /methanol for 30 minutes, followed by incubation in normal goat serum for 30 minutes at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Topical application of glycyrrhetinic acid in the gingival sulcus inhibits attachment loss in lipopolysaccharide‐induced experimental periodontitis in rats

Takamori

Yoshinaga

Ukai

et al. 2018

J of Periodontal Research

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“…Bacterial biofilm attaches to the tooth surface, making it impossible for the immune system to eradicate the infecting microorganisms efficiently, perpetuating the insult to the periodontal tissues [18]. The junctional epithelium is the first periodontal structure to face the bacterial challenge [23]. Bacteria are capable to cross the junctional epithelium and pass to the gingival conjunctive tissue, where they stimulate the gingival epithelial cells and fibroblasts to trigger the initial inflammatory responses [24].…”

Section: Innate Immune Response In Periodontal Diseasementioning

confidence: 99%

Pathogenesis of Periodontal Disease

Muñoz‐Carrillo¹,

Hernández-Reyes²,

García-Huerta³

et al. 2020

Periodontal Disease - Diagnostic and Adjunctive Non-Surgical Considerations

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Inflammation is a physiological response of the innate immune system against several endogenous or exogenous stimuli. Inflammation begins with an acute pattern; however, it can become chronic by activating the adaptive immune response through cellular and noncellular mechanisms. The main etiologic factor of periodontal disease is bacteria which substantially harbor the human oral cavity. The most common periodontal diseases are gingivitis and periodontitis, whose main characteristic is inflammation. The knowledge of how immune mechanisms and inflammatory responses are regulated is fundamental to understanding the pathogenesis of periodontal disease. The purpose of this chapter is to show the current panorama of the immunological mechanisms involved in the pathogenesis of periodontal disease.

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The histopathological comparison on the destruction of the periodontal tissue between normal junctional epithelium and long junctional epithelium

Abstract: Our findings suggest that the destruction of periodontal tissue is increased in tissue containing long junctional epithelium compared with normal junctional epithelium and that the immunized condition accelerates the destruction by forming immune complexes.

Cited by 26 publications

References 32 publications

Differences in mucin expression in the submandibular glands of rats during peridontitis induction

Differences in mucin expression in the submandibular glands of rats during peridontitis induction

Topical application of glycyrrhetinic acid in the gingival sulcus inhibits attachment loss in lipopolysaccharide‐induced experimental periodontitis in rats

Pathogenesis of Periodontal Disease

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