In Guinea-Bissau, Portuguese is the only official language, and, for historical and sociopolitical reasons, European Portuguese (EP) is assumed as the variety spoken. However, we find a multilingual space in which Kriol is the national unity language and Portuguese is spoken as a second language. In this context, we analyzed the intonation of neutral yes-no questions of Guinea-Bissau Portuguese (GBP), to examine whether the intonation of this sentence type resembles that found SEP (spoken in Lisbon) or whether we are facing a Guinea-Bissau Portuguese variety in formation. Using the theoretical framework of Prosodic Phonology and Intonational Phonology, we analyzed neutral yes-no questions produced through a reading task performed by four Guinea-Bissau participants, native speakers of Kriol, and speakers of Portuguese as a second language. Our results show that, for the pre-nuclear and nuclear contour as well as tonal density, the intonation of GBP neutral yes-no questions differs from SEP and is closer to Brazilian and African varieties already studied. This outcome was previously pointed out in the literature for declarative sentences, suggesting that GBP is developing its own intonational grammar.