The history of selenium—vitamin E interrelationships

Moore, Thomas

doi:10.1079/pns19620029

Cited by 12 publications

(2 citation statements)

References 31 publications

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“…The negative results presented here are in general agreement with those of Moore ( 15) who was unable to show any statistically significant difference in occurFence of tumors in mice which had received human malignant tissue extracts compared with controls. As in the in vitro studies( 1) reported previously, the negative findings recorded here should not be construed as positive evidence for a nonviral etiology in malignancies of the human species.…”

Section: ) In Similar Investigations Withsupporting

confidence: 92%

Search for Virus in Human Malignancies. 2. In vivo Studies.

Girardi¹,

Hilleman²,

Zwickey³

1962

Experimental Biology and Medicine

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The findings in studies to recover viruses in cell culture systems from human malignant tissues have been recorded ( 1). This in vitro work was paralleled by in vivo studies in which newborn mice and hamsters were employed as the indicator system. The results obtained in the latter study are presented here.Materials and methods. The collection and processing of clinical specimens and methods for preparation of tissue cultures from malignant tissues have been described( 1 ) . Mice of the ICR/Ha strain were maintained in these laboratories as a random bred strain. For breeding purpose, one male and one female were held as a monogamous family unit from 8 weeks to 10 months of age. The young were weaned at 21 days. The feed consisted of high fat (11%) breeder diet (Ralston Purina). The colony routinely proved free of Salmonella, ectromelia and other common mouse pathogens, and special work carried out in our laboratory indicated freedom from PVM and LCM viruses. The experimental mice and 700 breeder mice were tested and found free of hemagglu tination-inhibi ting (HI) antibodies for polyoma virus. Pregnant hamsters were obtained from Lakeview Hamster Colony, Newfield, N. J. During experi-*This work was supported in part by grant from U. S. Public Health Service. mental use, the animal diet consisted of pelleted Rockland Mouse Diet supplemented twice weekly with green peanuts and kale or carrots. Animal inoculation and observation. Newborn mice less than 2 0 hours old were inoculated subcutaneously in the dorsal nuchal area with 0.05 to 0.1 ml of material. The mice were weaned at 21 days of age and the mother was discarded. Hamsters less than 20 hours of age were inoculated subcutaneously with 0.1 to 0.2 ml of sample. The animals were weaned at 18 to 2 1 days of age, and the mothers were discarded after bleeding for tests for polyoma antibody. During the first 60 to 90 days, each litter of mice or hamsters was housed individually in "sealed" filter cages(2) of special design which precluded airborne cross-infection between cages. All manipulations, such as feeding and palpation of animals, were performed on the individual cages inside a hood equipped with ultraviolet light, continuous exhaust, entrance and exit locks, and rubber sleeves and gloves. Autoclaved water bottles and water were used and the tube was inserted into the cage through a tight-fitting aperture. After the early critical 2-or 3-month period, animals were transferred to ordinary holding boxes with mesh screen lids. The sexes were not segregated. The animals were palpated at least once each week and, in addition, each cage was examined twice daily for dead animals,

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