The history of the Ziehl-Neelsen stain

Bishop, Patrick; Neumann, Gerhard

doi:10.1016/0041-3879(70)90073-5

Cited by 68 publications

(35 citation statements)

References 7 publications

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“…Staining was performed according to standard protocol (6). Briefly, bacteria were heat fixed to a glass microscope slide and then stained with carbolfuchsin (Newcomer Supply) by gentle heating until steam was observed for a total of 5 min, washed thoroughly with water, and decolorized with Bacto TB decolorizer.…”

Section: Methodsmentioning

confidence: 99%

Lipoarabinomannan Localization and Abundance during Growth of Mycobacterium smegmatis

et al. 2011

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Lipoarabinomannan (LAM) is a structurally heterogeneous amphipathic lipoglycan present in Mycobacterium spp. and other actinomycetes, which constitutes a major component of the cell wall and exhibits a wide spectrum of immunomodulatory effects. Analysis of Mycobacterium smegmatis subcellular fractions and spheroplasts showed that LAM and lipomannan (LM) were primarily found in a cell wall-enriched subcellular fraction and correlated with the presence (or absence) of the mycolic acids in spheroplast preparations, suggesting that LAM and LM are primarily associated with the putative outer membrane of mycobacteria. During the course of these studies significant changes in the LAM/LM content of the cell wall were noted relative to the age of the culture. The LAM content of the M. smegmatis cell wall was dramatically reduced as the bacilli approached stationary phase, whereas LM, mycolic acid, and arabinogalactan content appeared to be unchanged. In addition, cell morphology and acid-fast staining characteristics showed variations with growth phase of the bacteria. In the logarithmic phase, the bacteria were found to be classic rod-shaped acid-fast bacilli, while in the stationary phase M. smegmatis lost the characteristic rod shape and developed a punctate acid-fast staining pattern with carbolfuchsin. The number of viable bacteria was independent of LAM content and phenotype. Taken together, the results presented here suggest that LAM is primarily localized with the mycolic acids in the cell wall and that the cellular concentration of LAM in M. smegmatis is selectively modulated with the growth phase.

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Section: Methodsmentioning

confidence: 99%

Lipoarabinomannan Localization and Abundance during Growth of Mycobacterium smegmatis

et al. 2011

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“…Edward's diagnosis of tuberculosis tumor was made possible due to the knowledge of bacterial infection and the histopathological techniques available at the time. It can be assumed that the method used by Thacher was the Ziehl-Neelsen stain (see Bishop & Neumann, 1970, for a history of this staining method). However, the type of staining procedure used was not specified in his report.…”

Section: Assessment and Treatment Of Tuberculosis In The Nineteenth Cmentioning

confidence: 99%

Aphasia Secondary to Tuberculosis: A Review of a Nineteenth-Century Case Report by Booth and Curtis (1893)

Shafi

2014

Journal of the History of the Neurosciences

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The topic of aphasia secondary to tuberculosis deserves attention for two reasons: first, for better understanding rare etiologies of aphasia in medical history; and secondly, for initiating a multidisciplinary discussion relevant to aphasiologists, neurologists, pathologists, and clinicians generally. This article will focus on clinical observations of tuberculosis-related aphasia in the nineteenth century, highlighting a noteworthy case report presented by Booth and Curtis (1893).

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“…The first two serial sections were subjected to haematoxylin and eosin, and acid-fast staining, respectively. Site A samples were stained using standard Ziehl-Neelsen (ZN) method for tissue by the pathology laboratory of Montreal General Hospital [16,17]. Site B samples were subjected to Fite's stain, a laboratory variant of the ZN method for tissue samples [18].…”

Section: Clinical Tissue Samples and Blindingmentioning

confidence: 99%

Visualization of Mycobacterium avium in Crohn's tissue by oil-immersion microscopy

Jeyanathan

Boutros-Tadros

Radhi

et al. 2007

Microbes and Infection

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The history of the Ziehl-Neelsen stain

Cited by 68 publications

References 7 publications

Lipoarabinomannan Localization and Abundance during Growth of Mycobacterium smegmatis

Lipoarabinomannan Localization and Abundance during Growth of Mycobacterium smegmatis

Aphasia Secondary to Tuberculosis: A Review of a Nineteenth-Century Case Report by Booth and Curtis (1893)

Visualization of Mycobacterium avium in Crohn's tissue by oil-immersion microscopy

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