The HLA-A mutation assay: Improved technique and normal results

McCarron, M.A.; Kutlaca, A.; Morley, Alexander A.

doi:10.1016/0165-7992(89)90118-8

Cited by 36 publications

(11 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The assay is based on immunoselection of lymphocytes, which have phenotypically lost one hla-a allele, and cloning of such cells at limiting dilution (McCarron et al 1989, see fig. The hlu-a genes encode multiallelic cell-surface antigens whose presence can be detected by allele-specific antibodies.…”

Section: The Human Leucocyie Antigen-a ( H L a -A ) Assaymentioning

confidence: 99%

Somatic Mutation Detection in Human Biomonitoring

Olsen

Nielsen

Nexø

et al. 1996

Pharmacology & Toxicology

View full text Add to dashboard Cite

Somatic cell gene mutation arising in vivo may be considered to be a biomarker for genotoxicity. Assays detecting mutations of the haemoglobin and glycophorin A genes in red blood cells and of the hypoxanthine-guanine phosphoribosyltransferase and human leucocyte antigenes in T-lymphocytes are available in humans. This MiniReview describes these assays and their application to studies of individuals exposed to genotoxic agents. Moreover, with the implementation of techniques of molecular biology mutation spectra can now be defined in addition to the quantitation of in vivo mutant frequencies. We describe current screening methods for unknown mutations, including the denaturing gradient gel electrophoresis, single strand conformation polymorphism analysis, heteroduplex analysis, chemical modification techniques and enzymatic cleavage methods. The advantage of mutation detection as a biomarker is that it integrates exposure and sensitivity in one measurement. With the analysis of mutation spectra it may thus be possible to identify the causative genotoxic agent.

show abstract

Section: The Human Leucocyie Antigen-a ( H L a -A ) Assaymentioning

confidence: 99%

Somatic Mutation Detection in Human Biomonitoring

Olsen

Nielsen

Nexø

et al. 1996

Pharmacology & Toxicology

View full text Add to dashboard Cite

show abstract

“…Hypoxanthine-guanine phosphoribosyltransferase (hprt) and hla-a mutants can be detected in lymphocyte preparations [Albertini et al, 1982;Morley et al, 1983;McCarron et al, 1986;Janatipour et al, 1988]. Mutants at the glycophorin A locus (gpa) can be detected in peripheral blood red cell samples [Langlois et al, 1986[Langlois et al, , 1990Grant and Bigbee, 1993;Jensen and Bigbee, 1996].…”

Section: Introductionmentioning

confidence: 98%

Estimation of mutation rate at human glycophorin A locus in hematopoietic stem cell progenitors

Vickers

Hoy

Lake

et al. 2002

Environ and Mol Mutagen

View full text Add to dashboard Cite

Surveys of human mutant cells exhibit a few individuals with relatively high "outlying" values, which might be explained by rare mutations occurring during development. To estimate how commonly this occurs, mutant red cell frequencies at the glycophorin A locus in 135 neonates and 109 children and adolescents from three research centers are compared with simulations in which mutations arise from successive cycles of binary fission. The simulations predict the data most accurately when the mutation rate in stem cell precursors is about 2-4 x 10(-7) per division cycle, which is similar to previous estimates from adult stem cell divisions. If these mutation rates are accurate, and the number of stem cell divisions during adult life is as low as previously estimated, it is predicted that up to one-sixth of mutant stem cells over a lifetime arose in early life. However, these mutant stem cells would be difficult to detect in surveys because their distribution within the general population is so skewed.

show abstract

“…The recently developed HLA-A assay (25) is performed on white blood cells, which contain DNA, and therefore the assay can be used to provide detailed information on mutational mechanism. HLA-A is a surface protein found on most nucleated cells in the body and is involved in the immune response and self versus nonself discrimination (26).…”

Section: Hla-a Assaymentioning

confidence: 99%

“…The GPA assay has been applied to radiation and chemotherapy-exposed individuals, persons with occupational exposures to styrene, persons with cancerprone syndromes, and a limited general population (23,24,32,(57)(58)(59)(60)(61)(62)(63). The HLA-A assay has been applied to the study of the effects of age and to chemotherapy-exposed individuals (25,27,64,65). The Hb-S assay has been applied to a small number of subjects with different mutagenic exposures (28) and with cancer-prone syndromes.…”

Section: Practical Considerations In Using These Assays In Epidemiolomentioning

confidence: 99%

Human Somatic Mutation Assays As Biomarkers of Carcinogenesis

Compton¹,

Hooper²,

Smith³

1991

Environmental Health Perspectives

View full text Add to dashboard Cite

lhis paper describes fourassaysthat detect somatic genemu in humans: thehypanthine-guin phosphoribosyl transferase assay, the glycophorin A assay, the HLA-A assay, and the sickle cell hemoglobin assay. Somatic gene mutation can be considered a bionarker ofcarciogenesi, andassays for somatic mutationm y assist epidemiologists in studies that attempt to identify factors associated with increased risks ofcancer. Practcal aspects ofthe use of these assays are discussed.

show abstract

The HLA-A mutation assay: Improved technique and normal results

Cited by 36 publications

References 13 publications

Somatic Mutation Detection in Human Biomonitoring

Somatic Mutation Detection in Human Biomonitoring

Estimation of mutation rate at human glycophorin A locus in hematopoietic stem cell progenitors

Human Somatic Mutation Assays As Biomarkers of Carcinogenesis

Contact Info

Product

Resources

About