The HLDA8 blind panel: Findings and conclusions

Swart, Bernadette; Salganik, M.P.; Wand, M. P.; Tinckam, Kathryn; Milford, Edgar L.; Drbal, Karel; Angelisová, Pavla; Hořejšı́, Václav; Macardle, Peter J.; Bailey, Sheree; Hollemweguer, Enoc; Hodge, Greg; Nairn, Judith; Millard, Debbrah J.; Dağdevıren, Attıla; Dandie, GW; Zola, Heddy

doi:10.1016/j.jim.2005.07.003

Cited by 4 publications

(1 citation statement)

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“…Viable cell isolation by antibody labeling has been instrumental in the characterization of functional cell subsets of hematopoietic, neural, and other cell types (Lawson et al, 2007; Maric and Barker, 2004; Swart et al, 2005). Excluding the well-studied hematopoietic field, however, the introduction of new cell lineage markers has been disappointingly elusive.…”

Section: Introductionmentioning

confidence: 99%

Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers

Dorrell

Grompe

Pan

et al. 2011

Molecular and Cellular Endocrinology

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Tools permitting the isolation of live pancreatic cell subsets for culture and/or molecular analysis are limited. To address this, we developed a collection of monoclonal antibodies with selective surface labeling of endocrine and exocrine pancreatic cell types. Cell type labeling specificity and cell surface reactivity were validated on mouse pancreatic sections and by gene expression analysis of cells isolated using FACS. Five antibodies which marked populations of particular interest were used to isolate and study viable populations of purified pancreatic ducts, acinar cells, and subsets of acinar cells from whole pancreatic tissue or of alpha or beta cells from isolated mouse islets. Gene expression analysis showed the presence of known endocrine markers in alpha and beta cell populations and revealed that TTR and DPPIV are primarily expressed in alpha cells whereas DGKB and GPM6A have a beta cell specific expression profile.

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Section: Introductionmentioning

confidence: 99%