The homeodomain transcription factor Pitx3 facilitates differentiation of mouse embryonic stem cells into AHD2-expressing dopaminergic neurons

Chung, Sangmi; Hedlund, Eva; Hwang, Moon‐Hyon; Kim, D.W.; Shin, Byoung‐Soo; Hwang, Deuk-Soo; Kang, Un Jung; Isacson, Ole; Kim, Kwangsoo

doi:10.1016/j.mcn.2004.09.008

Cited by 134 publications

(112 citation statements)

References 43 publications

Supporting

Mentioning

106

Contrasting

Unclassified

Order By: Relevance

“…The mouse blastocyst-derived ES cell line J1 was obtained from ATCC (Rockland, MD), and was propagated and maintained as described previously (Chung et al, 2005). Briefly, undifferentiated mES cells were cultured on gelatin-coated dishes in DMEM supplemented with 2 mM glutamine (Life Technologies, Boone, NC), 0.001% β-mercaptoethanol (Life Technologies), 1× nonessential amino acids (Life Technologies), 10% donor horse serum (Sigma), and 2000 U/ml human recombinant leukemia inhibitory factor (LIF; Invitrogen).…”

Section: Cell Culture and Neuronal Differentiation Of Mes Cellsmentioning

confidence: 99%

Induction of unfolded protein response during neuronal induction of rat bone marrow stromal cells and mouse embryonic stem cells

Cho

Jang

et al. 2009

Exp Mol Med

Self Cite

View full text Add to dashboard Cite

When we treated rat bone marrow stromal cells (rBMSCs) with neuronal differentiation induction media, typical unfolded protein response (UPR) was observed. BIP/GRP78 protein expression was time-dependently increased, and three branches of UPR were all activated. ATF6 increased the transcription of XBP1 which was successfully spliced by IRE1. PERK was phosphorylated and it was followed by eIF2α phosphorylation. Transcription of two downstream targets of eIF2α, ATF4 and CHOP/GADD153, were transiently up-regulated with the peak level at 24 h. Immunocytochemical study showed clear coexpression of BIP and ATF4 with NeuN and Map2, respectively. UPR was also observed during the neuro-

show abstract

Section: Cell Culture and Neuronal Differentiation Of Mes Cellsmentioning

confidence: 99%

Induction of unfolded protein response during neuronal induction of rat bone marrow stromal cells and mouse embryonic stem cells

Cho

Jang

et al. 2009

Exp Mol Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several developmental genes, such as Engrailed (En) 1 and 2, and Lmx1b, and cellular proteins such as aldehyde dehydrogenase-1 (Aldh1, also known as AHD-2), have been shown to be expressed in DA neuron precursors in the developing VM. However, the expression of these proteins is not restricted to the precursor pool, but is maintained also in the differentiated, post-mitotic mesDA neurons (9,14,21,30,32,37). Here we report that the proneural gene product Neurogenin2 (Ngn2) within the VM is expressed exclusively in the part of the proliferating ventricular zone (VZ), which gives rise to the migrating mesDA neuron precursors, but not in the differentiated, tyrosine hydroxylase (TH)-expressing postmitotic mesDA neurons.…”

Section: Introductionmentioning

confidence: 98%

Neurogenin2 identifies a transplantable dopamine neuron precursor in the developing ventral mesencephalon

Thompson

Andersson

Jensen

et al. 2006

Experimental Neurology

View full text Add to dashboard Cite

“…Pitx3 is a member of the pituitary family of bicoid type homeobox transcription factors (Semina et al, 1997). It is expressed in midbrain dopaminergic neurons by E11.5 (Smidt et al, 1997), where it facilitates differentiation of the A9 cell group constituting the substantia nigra pars compacta (SNc; Chung et al, 2005). In homozygous ak mice, genomic deletions within the promoter and exon1 of the Pitx3 gene (Rieger et al, 2001;Semina et al, 2000) result in incomplete lens development and aphakia.…”

Section: Introductionmentioning

confidence: 99%

Motor deficits and altered striatal gene expression in aphakia (ak) mice

et al. 2007

View full text Add to dashboard Cite

Like humans with Parkinsons disease (PD), the ak mouse lacks the majority of the substantia nigra pars compacta (SNc) and experiences striatal denervation. The purpose of this study was to test whether motor abnormalities in the ak mouse progress over time, and whether motor function could be associated with temporal alterations in the striatal transcriptome. Ak and wt mice (28 to 180 days old) were tested using paradigms sensitive to nigrostriatal dysfunction. Results were analyzed using a linear mixed model. Ak mice significantly underperformed wt controls in rotarod, balance beam, string test, pole test and cotton shred tests at all ages examined. Motor performance in ak mice remained constant over the first 6 months of life, with the exception of the cotton shred test, in which ak mice exhibited marginal decline in performance. Dorsal striatal semi-quantitative RT-PCR for 19 dopaminergic, cholinergic, glutaminergic and catabolic genes was performed in 1 and 6 month old groups of ak and wt mice. Preproenkephalin levels in ak mice were elevated in both age groups. Drd1, 3 and 4 levels declined over time, in contrast to increasing Drd2 expression. Additional findings included decreased Chrnα6 expression and elevated VGluT1 expression at both time points in ak mice, and elevated AchE expression in young ak mice only. Results confirm that motor ability does not decline significantly for the first 6 months of life in ak mice. Their striatal gene expression patterns are consistent with dopaminergic denervation, and change over time, despite relatively unaltered motor performance.

show abstract

The homeodomain transcription factor Pitx3 facilitates differentiation of mouse embryonic stem cells into AHD2-expressing dopaminergic neurons

Cited by 134 publications

References 43 publications

Induction of unfolded protein response during neuronal induction of rat bone marrow stromal cells and mouse embryonic stem cells

Induction of unfolded protein response during neuronal induction of rat bone marrow stromal cells and mouse embryonic stem cells

Neurogenin2 identifies a transplantable dopamine neuron precursor in the developing ventral mesencephalon

Motor deficits and altered striatal gene expression in aphakia (ak) mice

Contact Info

Product

Resources

About