The homing of human umbilical cord-derived mesenchymal stem cells and the subsequent modulation of macrophage polarization in type 2 diabetic mice

Yin, Yaqi; Hao, Haojie; Cheng, Yu; Gao, Jian; Liu, Jiejie; Xie, Zongyan; Zhang, Qi; Zang, Li; Han, Weidong; Mu, Yiming

doi:10.1016/j.intimp.2018.04.051

Cited by 46 publications

(31 citation statements)

References 42 publications

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“…Exogenous MSCs can also migrate to target tissues or organs to play a role in repair [34]. Several studies have demonstrated that the ability of MSCs to migrate to target tissues plays essential roles in many diseases [32,[35][36][37][38][39], such as breast cancer [35], ischemic kidney injury [36], bone healing [37], type 2 diabetes [38], and cardiac ischemia/perfusion injury [39]. In our study, we demonstrated that the combination of hUCMSCs or estrogen with a collagen scaffold enhanced HuNu and vimentin expression, suggesting that combination therapy can facilitate homing of stem cells in rats with IUA.…”

Section: Discussionmentioning

confidence: 99%

Collagen Scaffold with Human Umbilical Cord Mesenchymal Stem Cells Remarkably Improves Intrauterine Adhesions in a Rat Model

Liu

Cai

Wen

et al. 2020

Gynecol Obstet Invest

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Background: Intrauterine adhesion (IUA) is the second leading cause of secondary infertility in women. Research has shown that stem cells can promote endometrial regeneration and that biomaterials are also helpful in tissue regeneration. Therefore, we compared the efficacy of a collagen scaffold combined with either human umbilical cord mesenchymal stem cells (hUCMSC) or estrogen for the treatment of IUA. Methods: The IUA-induced rats were injected with hUCMSCs or estrogen, and with a collagen scaffold. The endometrial glands and amount of fibrosis were assessed using hematoxylin and eosin and Masson staining. The extent of fibrosis and levels of regeneration-related cytokines were examined by real-time quantitative PCR, and the expression levels of the estrogen receptor, KI67 and cytokeratin were analyzed using an immunochemistry assay. In addition, human nuclear antigen (HuNu) and vimentin were examined by immunofluorescence microscopy. Results: The collagen scaffold administered with hUCMSCs markedly increased the number of endometrial glands and reduced the area of fibrosis compared with either the collagen scaffold or hUCMSCs alone. In addition, the collagen scaffold with hUCMSCs significantly regulated the expression levels of fibrosis, estrogen, and differentiation-related genes relative to the collagen scaffold or hUCMSCs alone. Furthermore, the hUCMSCs alone or in combination with the collagen scaffold increased the expression of HuNu and vimentin in the IUA-induced rat model. In addition, protein levels of the p-transcriptional co-activator with PDZ-binding motif, stromal cell-derived factor-1, and C-X-C chemokine receptor type 4 were upregulated in the group that received the collagen scaffold in combination with hUCMSCs. Conclusion: Our results suggest that the combination of the collagen scaffold with hUCMSCs may be an alternative approach for treating IUA.

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Section: Discussionmentioning

confidence: 99%

Collagen Scaffold with Human Umbilical Cord Mesenchymal Stem Cells Remarkably Improves Intrauterine Adhesions in a Rat Model

Liu

Cai

Wen

et al. 2020

Gynecol Obstet Invest

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“… 12 The homing capacity of MSCs allows them to migrate to sites of injury and inflammation. 13 Several studies have demonstrated that after intravenous delivery, BM-MSCs migrate to the site of bone or cartilage damage, myocardial infarction, and ischemic cerebral injury. 14 – 16 Although MSCs were initially shown to promote tissue repair by trans-differentiation of specific cell types required to repair damaged tissue, current data suggest paracrine signaling as the primary mediator of the reparative effects of MSCs.…”

Section: Introductionmentioning

confidence: 99%

<p>Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats</p>

Wei

Zhang

et al. 2020

IJN

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Introduction: Mesenchymal stem cells (MSCs) are a promising resource for tissue regeneration and repair. However, their clinical application is hindered by technical limitations related to MSC enrichment at the target sites. Methods: MSCs were labeled with magnetic Fe 3 O 4 nanoparticles (NPs). We analyzed the effects of NP on cell proliferation, stem cell characteristics, and cytokine secretion. Furthermore, we induced NP-labeled MSC migration with an external magnetic field toward laser-induced skin wounds in rats and evaluated the associated anti-inflammatory effects. Results: Fe 3 O 4 NP application did not adversely affect MSC characteristics. Moreover, Fe 3 O 4 NP-labeled MSCs presented increased anti-inflammatory cytokine and chemokine production compared with unlabeled MSCs. Furthermore, MSCs accumulated at the injury site and magnetic targeting promoted NP-labeled MSC migration toward burn injury sites in vivo. On day 7 following MSC injection, reduced inflammation and promoted angiogenesis were observed in the magnetically targeted MSC group. In addition, anti-inflammatory factors were upregulated, whereas pro-inflammatory factors were downregulated within the magnetically targeted MSC group compared with those in the PBS group. Conclusion: This study demonstrates that magnetically targeted MSCs contribute to cell migration to the site of skin injury, improve anti-inflammatory effects and enhance angiogenesis compared with MSC injection alone. Therefore, magnetically targeted MSC therapy may be an effective treatment approach for epithelial tissue injuries.

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“…As for the therapeutic efficacy, various mechanisms have been proposed by which WJ-MSCs could mediate their observed beneficial effects in T1DM/T2DM [46]. Briefly, WJ-MSCs have the ability for "homing" to sites of tissue injury and secrete multiple bioactive mediators which are capable of stimulating recovery of injured cells, as well as various immuno-modulatory functions [52,53], and to a lesser extent, the observed improvement in C-peptide levels might also be attributed to their differentiation potential into insulin-producing β cells [54][55][56]. On the other hand, the putative therapeutic potential of UCB for DM was originally based on their proposed immunomodulatory actions especially for T1DM being an autoimmune disease [57].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic efficacy of umbilical cord-derived stem cells for diabetes mellitus: a meta-analysis study

Kassem

Kamal

2020

Stem Cell Res Ther

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Background Stem cell therapy provides great hope for patients with diabetes mellitus (DM). DM is a seriously alarming metabolic disease characterized by hyperglycemia and β cell dysfunction. Efficient novel therapeutic modalities to treat DM are indeed warranted. Stem cells (SC) derived from the umbilical cord specifically provide several advantages and unique characteristics being a readily available non-invasive source, with an additional credit for their banking potential. This meta-analysis study aims to provide a focused assessment for therapeutic efficacy of umbilical cord (UC)-derived SC-transplantation, namely Wharton’s jelly mesenchymal stem cells (WJ-MSCs) and umbilical cord blood (UCB) for DM. Methods The clinical efficacy was evaluated based on glycemic control status (reflected on HbA1c%) and β cell function (reflected on C-peptide levels), as well as the daily insulin requirement in diabetic patients after receiving UC-derived SC-transplantation compared to baseline values. Moreover, we assessed these outcome measures in patients who received such intervention compared to those who did not receive it in randomized/non-randomized controlled clinical trials. We employed a random-effects model and standardized mean difference for this meta-analysis. Results Eleven eligible clinical studies were included; WJ-MSCs (6 studies; 172 patients including 71 controls) and UCB (5 studies; 74 patients including 15 controls). WJ-MSCs significantly improved HbA1c% (pooled-estimate − 1.085; 95%CI (− 1.513, − 0.657); p < 0.001) and C-peptide levels (pooled-estimate 1.008; 95%CI (0.475, 1.541); p < 0.001), as well as the daily insulin-requirement (pooled-estimate − 2.027; 95%CI (− 3.32, − 0.733); p = 0.002). On the contrary, UCB was found to be uniformly ineffective; HbA1c% (pooled-estimate − 0.091, 95%CI (− 0.454, 0.271); p = 0.622), significantly deteriorated C-peptide levels (pooled-estimate − 0.789; 95%CI (− 1.252, − 0.325); p < 0.001) and daily insulin-requirement (pooled-estimate 0.916; 95%CI (0.247, 1.585); p = 0.007). All these observations remained consistent when we carried out sub-group meta-analysis for T1DM and T2DM and also when we compared patients who received WJ-MSCs or UCB to controls. Conclusions The results of our meta-analysis provide a clear evidence for the superior efficacy of WJ-MSCs over UCB in DM. This sheds lights on the importance to consider banking of WJ-MSCs together with the well-established routine UCB-banking, especially for those with family history of DM. Additionally, further clinical studies are required to investigate therapeutic efficacy of selected/enriched UCB-derived cell populations with immunomodulatory/regenerative potential in DM.

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The homing of human umbilical cord-derived mesenchymal stem cells and the subsequent modulation of macrophage polarization in type 2 diabetic mice

Cited by 46 publications

References 42 publications

Collagen Scaffold with Human Umbilical Cord Mesenchymal Stem Cells Remarkably Improves Intrauterine Adhesions in a Rat Model

Collagen Scaffold with Human Umbilical Cord Mesenchymal Stem Cells Remarkably Improves Intrauterine Adhesions in a Rat Model

<p>Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats</p>

Therapeutic efficacy of umbilical cord-derived stem cells for diabetes mellitus: a meta-analysis study

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