The hormonal and neural control of egg production in the historically important model insect, Rhodnius prolixus: A review, with new insights in this post-genomic era

Lange, Angela B.; Leyria, Jimena; Orchard, Ian

doi:10.1016/j.ygcen.2022.114030

Cited by 18 publications

(38 citation statements)

References 113 publications

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“…Given the rise in insecticide‐resistant vectors, the development of innovative control strategies relies on a thorough understanding of vector biology and pathogen transmission (Wilson et al, 2020). In this context, targeting the molecular mechanisms of vectors reproduction emerges as a promising approach to allow specific interventions on oocyte formation and reproduction in species that transmit human diseases such as R. prolixus (Lange et al, 2022; Nunes‐da‐Fonseca et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Knockdown of E1‐ and E2‐ubiquitin enzymes triggers defective chorion biogenesis and modulation of autophagy‐related genes in the follicle cells of the vector Rhodnius prolixus

Pereira

Dias

Ramos

2022

Journal Cellular Physiology

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In insects, the last stage of oogenesis is the process where the chorion layers (eggshell) are synthesized and deposited on the surface of the oocytes by the follicle cells. Protein homeostasis is determined by the fine‐tuning of translation and degradation pathways, and the ubiquitin–proteasome system is one of the major degradative routes in eukaryotic cells. The conjugation of ubiquitin to targeted substrates is mediated by the ordered action of E1‐activating, E2‐conjugating, and E3‐ligase enzymes, which covalently link ubiquitin to degradation‐targeted proteins delivering them to the proteolytic complex proteasome. Here, we found that the mRNAs encoding polyubiquitin (pUbq), E1, and E2 enzymes are highly expressed in the ovaries of the insect vector of Chagas Disease Rhodnius prolixus. RNAi silencing of pUbq was lethal whereas the silencing of E1 and E2 enzymes resulted in drastic decreases in oviposition and embryo viability. Eggs produced by the E1‐ and E2‐silenced insects presented particular phenotypes of altered chorion ultrastructure observed by high‐resolution scanning electron microscopy as well as readings for dityrosine cross‐linking and X‐ray elemental microanalysis, suggesting a disruption in the secretory routes responsible for the chorion biogenesis. In addition, the ovaries from silenced insects presented altered levels of autophagy‐related genes as well as a tendency of upregulation in ER chaperones, indicating a disturbance in the general biosynthetic‐secretory pathway. Altogether, we found that E1 and E2 enzymes are essential for chorion biogenesis and that their silencing triggers the modulation of autophagy genes suggesting a coordinated function of both pathways for the progression of choriogenesis.

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Section: Introductionmentioning

confidence: 99%

Knockdown of E1‐ and E2‐ubiquitin enzymes triggers defective chorion biogenesis and modulation of autophagy‐related genes in the follicle cells of the vector Rhodnius prolixus

Pereira

Dias

Ramos

2022

Journal Cellular Physiology

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“…The molecular physiology of oogenesis is highly conserved within the different insect vectors [11][12][13]. In brief, oogenesis is triggered by signals from nutritional status and the blood meal.…”

Section: Reproduction As a Target For Vector Controlmentioning

confidence: 99%

“…Major advancements have been achieved in the understanding of the neurohormonal control of egg production. In R. prolixus, a detailed model depicting the control of oogenesis, ovulation, and oviposition has been designed and elegantly reviewed by Lange and colleagues [13]. Post-genomics has allowed the identification and functional characterization of dozens of reproduction-related neuropeptide receptor families, processing enzymes, and neurochemicals.…”

Section: Molecular Neuroendocrine Control Of Egg Productionmentioning

confidence: 99%

Vector Control: Insights Arising from the Post-Genomics Findings on Insects’ Reproductive Biology

Ramos¹,

Gomes²

2023

New Advances in Neglected Tropical Diseases

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The high prevalence of neglected vector-borne diseases, such as Chagas disease and dengue fever, imposes enormous health and financial burdens in developing countries. Historically, and still, to this day, the main effective methods to manage those diseases rely on vector population control. Although early efforts in understanding vector-specific biology resulted in important advancements in the development of strategies for the management of vector-borne diseases, studies regarding the complex physiology of local vector species were weakened by the expanding use of insecticide-based tools, which were, at the time, proven simpler and effective. The rising threat of insecticide resistance and climate change (which can expand endemic areas) has reemphasized the need to rely on thorough species-specific vector biology. One approach to controlling vector populations is to disrupt molecular processes or antagonize the metabolic targets required to produce viable eggs. Here, we discuss new findings arising from post-genomics molecular studies on vector reproductive biology and discuss their potential for the elaboration of new effective vector control interventions.

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“…These biogenic amines directly regulate oogenesis and oviposition in a range of insects (e.g. 10 , 16 , 17 , 18 ) and also indirectly affect oogenesis by regulating JH titres 19 , 20 , 21 .…”

Section: Neuroendocrine Control Of Reproduction In Female Insectsmentioning

confidence: 99%

Environmentally responsive reproduction: neuroendocrine signalling and the evolution of eusociality

Knapp¹,

Norman²,

Rouse³

et al. 2022

Current Opinion in Insect Science

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The hormonal and neural control of egg production in the historically important model insect, Rhodnius prolixus: A review, with new insights in this post-genomic era

Cited by 18 publications

References 113 publications

Knockdown of E1‐ and E2‐ubiquitin enzymes triggers defective chorion biogenesis and modulation of autophagy‐related genes in the follicle cells of the vector Rhodnius prolixus

Knockdown of E1‐ and E2‐ubiquitin enzymes triggers defective chorion biogenesis and modulation of autophagy‐related genes in the follicle cells of the vector Rhodnius prolixus

Vector Control: Insights Arising from the Post-Genomics Findings on Insects’ Reproductive Biology

Environmentally responsive reproduction: neuroendocrine signalling and the evolution of eusociality

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