The hormonal coordination of cuticulin deposition and morphogenesis in Drosophila imaginal discs in vivo and in vitro

Fristrom, Dianne; Liebrich, Winfrid

doi:10.1016/0012-1606(86)90378-7

Cited by 36 publications

(16 citation statements)

References 17 publications

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“…The adult cuticle eventually forms under the pupal cuticle so that prior to eclosion the organism is enclosed within three cuticles. The formation of the pupal epicuticle starts about 2 h [15] and the procuticle about 9 h after puparium formation at 25°C [ll] (Fig. 1).…”

Section: Pupal Cuticle Formation In Vivomentioning

confidence: 99%

Ecdysone regulation of cuticle protein gene expression inDrosophila

Fristrom

Alexander

Brown

et al. 1986

Arch. Insect Biochem. Physiol.

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The deposition and apolysis of insect cuticles have long been known to be regulated by ecdysone. Unsclerotized, chitin-containing procuticles contain evolutionarily conserved, hydrophobic proteins that are soluble in solutions of denaturing agents. The pupal procuticle of Drosophila is deposited by larval and imaginal epidermis starting 9 h after puparium formation when the ecdysone titer is low. Initially, a set of low molecular weight proteins (less than 25,000 daltons; low molecular weight pupal cuticle proteins = S-PCPs) is synthesized. However, about the time of pupation, synthesis of S-PCPs ceases, and high molecular weight proteins (greater than 50,000 daltons; HPCPs) are synthesized. In vitro experiments indicate that the initial formation of the procuticle with synthesis of the S-PCPs requires a pulse of hormone followed by withdrawal (6 h with 20-hydroxyecdysone, 1 pg/ml). The switch from synthesis of S-PCPs to H-PCPs is facilitated by a second, short pulse of 20-hydroxyecdysone (0.1 pglml, 3 h). Ultrastructural localization demonstrates that the S-PCPs are located only in the external lamellae of the procuticle, while the H-PCPs are present only in internal lamellae. Developmental analyses with cloned genes indicate that cuticle protein genes are expressed during only one stage of Drosophila development. Some of the genes encoding S-PCPs are limited in their expression to larval (posterior) or imaginal (anterior) epidermis. Preliminary molecular analyses of the larval and pupal cuticle protein genes indicate that they are organized in different ways. For example, four larval genes exist in a cluster with divergent transcription, and one PCP gene, PCP-CART, is located within an intron of a "housekeeping" gene.

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Section: Pupal Cuticle Formation In Vivomentioning

confidence: 99%

Ecdysone regulation of cuticle protein gene expression inDrosophila

Fristrom

Alexander

Brown

et al. 1986

Arch. Insect Biochem. Physiol.

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“…This morphogenesis involves cell rearrangement (Fristrom and kckoll, 1982) leading to evagination, during which epithelium shifts from its position within the thoracic cavity to the exterior surface. Pupal cuticle is the first cuticle produced by the wing disc epithelium (Doctor et al, 1985;Fristrom and Liebrich, 1986) and consists of two major components: an outer layer, the epicuticle, composed mainly of proteins without chitin, and an inner layer, the procuticle, which contains chitin in association with proteins.…”

Section: Introductionmentioning

confidence: 99%

Chitin synthesis in Spodoptera frugiperda wing imaginal discs. II. Selective action of chlorfluazuron on wheat germ agglutinin binding and cuticle ultrastructure

Zimowska

Mikołajczyk

Silhacek

et al. 1994

Arch Insect Biochem Physiol

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The action of the chitin synthesis inhibitor, chlorfluazuron, was investigated in Spodoptera frugiperda wing imaginal discs cultured in vitro. Electron microscopy and cytochemical labeling with a lectin, wheat germ agglutinin (WGA), were used to monitor morphogenesis, as well as the presence and localization of chitin and non-polymerized N-acetyl-D-glucosamine (GlcNAc). Chlorfluazuron (CFA) selectively inhibited 20-hydroxyecdysone-stimulated chitin synthesis and procuticle deposition in imaginal discs, without otherwise affecting their morphogenesis. Tracheole migration, evagination, exocytosis, and endocytosis in the epithelial cells, and the presence of non-polymerized GlcNAc in the extracellular matrix, were observed in both CFA-treated and control wing discs. O n the other hand, CFA prevented the appearance of WGA-labeled chitin in newly formed procuticle, while the deposition of proteinaceous cuticulin and epicuticle was unaffected. A brief treatment with CFA resulted in WGA labeling of non-polymerized GlcNAc, but not chitin in the procuticle region. The lack of chitin in CFA treated wing discs was correlated with the appearance of an amorphous, non-lamellar procuticle region. 0 1994 Wiley-Liss, Inc.'

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“…The regime of steroid administration that elicited DDC transcript accumulation in vitro was precisely that encountered by the discs in vivo. As was shown by Hodgetts et al [7l, the high titer of 20-OH-ecdysone reached at pupariation rapidly decreases thereafter, and by the time imaginal discs begin to produce the DDC required to sclerotize the pupal cuticle (the secretion of which begins 8 h after pupariation [21]) levels of 20-OH-ecdysone are near the minimum detectable level.…”

Section: ; IIImentioning

confidence: 59%