The host effects of Gambusia affinis with an antibiotic-disrupted microbiome

Carlson, Jeanette M.; Hyde, Embriette R.; Petrosino, Joseph F.; Manage, Ananda B. W.; Primm, Todd P.

doi:10.1016/j.cbpc.2015.10.004

Cited by 47 publications

(39 citation statements)

References 23 publications

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“…Excess antibiotics in the water may affect the natural composition of bacteria externally and internally in fish (Daughton and Brooks 2011, Carlson et al, 2015). Possible effects include the suppression of beneficial bacteria and enhancement of pathogenic bacterial resistance to antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Contaminants of emerging concern in a large temperate estuary

Meador

Yeh

Young

et al. 2016

Environmental Pollution

203

132

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This study was designed to assess the occurrence and concentrations of a broad range of contaminants of emerging concern (CECs) from three local estuaries within a large estuarine ecosystem. In addition to effluent from two wastewater treatment plants (WWTP), we sampled water and whole-body juvenile Chinook salmon (Oncorhynchus tshawytscha) and Pacific staghorn sculpin (Leptocottus armatus) in estuaries receiving effluent. We analyzed these matrices for 150 compounds, which included pharmaceuticals, personal care products (PPCPs), and several industrial compounds. Collectively, we detected 81 analytes in effluent, 25 analytes in estuary water, and 42 analytes in fish tissue. A number of compounds, including sertraline, triclosan, estrone, fluoxetine, metformin, and nonylphenol were detected in water and tissue at concentrations that may cause adverse effects in fish. Interestingly, 29 CEC analytes were detected in effluent and fish tissue, but not in estuarine waters, indicating a high potential for bioaccumulation for these compounds. Although concentrations of most detected analytes were present at relatively low concentrations, our analysis revealed that overall CEC inputs to each estuary amount to several kilograms of these compounds per day. This study is unique because we report on CEC concentrations in estuarine waters and whole-body fish, which are both uncommon in the literature. A noteworthy and unexpected finding was the preferential bioaccumulation of CECs in free-ranging juvenile Chinook salmon relative to staghorn sculpin, a benthic species with relatively high site fidelity.

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Section: Discussionmentioning

confidence: 99%

Contaminants of emerging concern in a large temperate estuary

Meador

Yeh

Young

et al. 2016

Environmental Pollution

203

132

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show abstract

“…This suggests that individual antibiotics affect a key function of the normal microbiome, namely colonization resistance, very differently. The controllable vertebrate model used in this study is now being used to investigate the effects on the host organism resulting from significant antibiotic-induced microbiome community changes,24,25 and driving factors during the recovery of the microbiome after antibiotic stress. A 3-day exposure of fish to rifampicin at the same concentration and same method used in this study resulted in reduced resistance to the pathogen Edwardsiella ictaluri , reduced weight gain over a month, and increased susceptibility to osmotic stress, compared to controls.…”

Section: Discussionmentioning

confidence: 99%

Microbiome disruption and recovery in the fish <em>Gambusia affinis</em> following exposure to broad-spectrum antibiotic

Carlson¹,

Leonard²,

Hyde

et al. 2017

IDR

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Antibiotics are a relatively common disturbance to the normal microbiota of humans and agricultural animals, sometimes resulting in severe side effects such as antibiotic-associated enterocolitis. Gambusia affinis was used as a vertebrate model for effects of a broad-spectrum antibiotic, rifampicin, on the skin and gut mucosal microbiomes. The fish were exposed to the antibiotic in the water column for 1 week, and then monitored during recovery. As observed via culture, viable counts from the skin microbiome dropped strongly yet returned to pretreatment levels by 1.6 days and became >70% resistant. The gut microbiome counts dropped and took longer to recover (2.6 days), and became >90% drug resistant. The resistance persisted at ~20% of skin counts in the absence of antibiotic selection for 2 weeks. A community biochemical analysis measuring the presence/absence of 31 activities observed a 39% change in results after 3 days of antibiotic treatment. The antibiotic lowered the skin and gut microbiome community diversity and altered taxonomic composition, observed by 16S rRNA profiling. A 1-week recovery period did not return diversity or composition to pretreatment levels. The genus Myroides dominated both the microbiomes during the treatment, but was not stable and declined in abundance over time during recovery. Rifampicin selected for members of the family Comamonadaceae in the skin but not the gut microbiome. Consistent with other studies, this tractable animal model shows lasting effects on mucosal microbiomes following antibiotic exposure, including persistence of drug-resistant organisms in the community.

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“…(48), starting from Eqs. (33) and (34), we will perform the integration, using two choices of Breit frames with the aim to cross check the method and its application. In a first choice, which we denote as P -frame, the kinematics of the external particles is defined by K = K 0 , 0, 0, | K| , P = P (1, 0, 0, 0) , q = Q (0, 1, 0, 0) .…”

Section: Evaluation Of the Inelastic Tpe Contributionmentioning

confidence: 99%

“…The importance of the qualitative and quantitative understanding of TPE effects is also necessary in view of the 4-7 standard deviations discrepancy in the proton rms charge radius extraction when comparing muonic hydrogen spectroscopy extractions [28,29] with experiments using electrons [21,24,[30][31][32]; see Ref. [33] for a recent review. The TPE corrections constitute the leading hadronic uncertainties in the extraction of the proton charge radius from muonic hydrogen spectroscopy.…”

mentioning

confidence: 99%

Two-photon exchange correction in elastic unpolarized electron-proton scattering at small momentum transfer

Tomalak

Vanderhaeghen

2016

Phys. Rev. D

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We evaluate the two-photon exchange (TPE) correction to the unpolarized elastic electron-proton scattering at small momentum transfer Q 2 . We account for the inelastic intermediate states approximating the double virtual Compton scattering by the unpolarized forward virtual Compton scattering. The unpolarized proton structure functions are used as input for the numerical evaluation of the inelastic contribution. Our calculation reproduces the leading terms in the Q 2 expansion of the TPE correction and goes beyond this approximation by keeping the full Q 2 dependence of the proton structure functions. In the range of small momentum transfer our result is in good agreement with the empirical TPE fit to existing data. Contents

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The host effects of Gambusia affinis with an antibiotic-disrupted microbiome

Cited by 47 publications

References 23 publications

Contaminants of emerging concern in a large temperate estuary

Contaminants of emerging concern in a large temperate estuary

Microbiome disruption and recovery in the fish <em>Gambusia affinis</em> following exposure to broad-spectrum antibiotic

Two-photon exchange correction in elastic unpolarized electron-proton scattering at small momentum transfer

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