The Hot‐Water Extract of Smilacis Chinae Rhizome Suppresses 2,4‐Dinitrochlorobenzene and House Dust Mite‐Induced Atopic Dermatitis‐Like Skin Lesions in Mice

Abstract

Smilacis Chinae Rhizome (SCR) has been used as an oriental folk medicine for various biological activities. However, its effect on atopic dermatitis (AD) remains undetermined to date. We assessed the effect of orally administered hot-water extract of SCR on AD-like skin lesions in mice and its underlying mechanisms. AD-like murine model was prepared by repeated alternate application of house dust mite (Dermatophagoides farinae) extract (DFE) and 2,4-dinitrochlorobenzene (DNCB) for 4 weeks, topically to the ears. Daily oral administration of SCR for 3 and 4 weeks significantly reduced inflammatory ear thickening, with the effect being enhanced at the earlier start and longer period of administration. This effect was accompanied by a significant decrease in both Th2 and Th1 serum antibodies (total IgE, DFE-specific IgE, and IgG2a). Histological analysis showed that SCR markedly decreased the epidermal/dermal ear thickening and the dermal infiltration of inflammatory cells. Furthermore, SCR suppressed DFE/DNCB-induced expression of IL-4, IL-13, IL-17, IL-18, TSLP, and IFN-γ genes in the ear tissue. Taken together, our observations demonstrate that chronic oral administration of SCR exerts beneficial effect in mouse AD model, suggesting that SCR has the therapeutic potential as an orally active treatment of AD by modulating both Th1 and Th2 responses.

Hydrogel-Based Treatment of House Dust Mite-Induced Atopic Dermatitis through Triple Cleaning of Mites, Bacteria, and ROS-Related Inflammation

“…In this study, HDM, the most common allergen in clinical settings, was used as the triggering antigen during the preparation of the AD mice model, which was coinduced with 1-chloro-2,4-dinitrobenzene (DNCB), allowing the animal model to better mimic the clinical disease. − Before applying the CPDP hydrogel to AD treatment, we initially explored its penetration efficiency into the mice’skin (Figure S15), which clarified that the hydrogel was able to penetrate about 23% within 5 h. In the animal experiments (Figure a), the mice were randomly divided into 5 groups, i.e. , Normal group, Model group, dexamethasone (DEX) group, CPDP– group, and CPDP+ group (“–” means without light irradiation, and “+” means 808 nm NIR irradiation).…”

Ethanolic Extracts of Artemisia apiacea Hance Improved Atopic Dermatitis-Like Skin Lesions In Vivo and Suppressed TNF-Alpha/IFN-Gamma–Induced Proinflammatory Chemokine Production In Vitro

“…Repeated cutaneous application of DNCB induces AD-like dermatitis in BALB/c mice. In AD-like skin by DNCB, many changes such as a thick epidermis of the epidermis and dermis, significant keratosis, infiltration of inflammatory cells, ulceration, and hemorrhage are observed [ 31 ]. We found that EAH restrained edema of the ear skin and restored epidermal thickness through the histological analysis ( Figure 5 ).…”

Inhibition of Th1 and Th17 Cells by Medicinal Plants and Their Derivatives: A Systematic Review

“…These plants can exert many biological effects. In several studies, their effects on prevention and treatment of different diseases have been reported (Kafash‐Farkhad et al , ; Bahmani et al , ; Saki et al , ; Asadbeigi et al , ; Asadi et al , ; Shirzad et al , ; Bahmani et al , ; Asadi‐Samani et al , ; Bahmani et al , ; Bahmani et al , ; Asadi‐Samani et al , ; Mahmoud et al , ; Ki et al , ; Talat et al , ). They are a rich source of natural compounds for preparation of safe and effective medications which can be used to treat inflammatory diseases.…”