The hsrω 05241 allele of the noncoding hsrω gene of Drosophila melanogaster is not responsible for male sterility as reported earlier

Akanksha, .; Mallik, Moushami; Fatima, Roshan; Lakhotia, S. C.

doi:10.1007/s12041-008-0012-8

Cited by 6 publications

(9 citation statements)

References 7 publications

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“…This line, like the parental one from which it was derived, was associated with male sterility in the homozygous state. As in case of the parental hsrω 05241 stock, 69 recessive male sterility in the derived EP93D line was also due to a second site mutation which was recombined out. Likewise, recessive embryonic lethality in the original EP3037 stock was also recombined out.…”

Section: Non-coding Hsrω Rna and Polyq Toxicity In Drosophilamentioning

confidence: 99%

RNAi for the large non-coding hsrω transcripts suppresses polyglutamine pathogenesis inDrosophilamodels

Mallik

Lakhotia²

2009

RNA Biology

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108

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Polyglutamine diseases are a class of inherited neurodegenerative disorders, characterized by expansion of CAG trinucleotide repeats translated into elongated glutamine tracts within the mutant proteins. Overexpression of the non-coding hsromega transcripts has been shown to dominantly enhance polyQ induced cytotoxicity in Drosophila. In the present study we demonstrate that RNA interference mediated downregulation of hsromega-n transcripts is sufficient to suppress pathogenesis in several Drosophila models of human polyQ neurodegenerative diseases. Loss of hsromega-n RNA not only suppresses the eye-specific degeneration mediated by GMR-GAL4 driven expression of the 127Q or MJDtr-Q78 or ataxin1 82Q or httex1p Q93 transgene, but also rescues premature death of flies expressing the expanded polyQ proteins pan-neuronally using the elav-GAL4 driver. We further demonstrate that the morphological and functional rescue of polyQ toxicity observed upon hsromega-n RNAi is associated with substantial reduction of polyQ protein aggregation without affecting transcription of the 127Q transgene. Unlike in the polyQ expressing cells, co-expression of hsromega-n RNAi also abolishes the induction of Hsp70. These results suggest that the hsromega transcripts have a role in early stages of polyQ aggregate formation. Interestingly, hsromega-RNAi has, at best, only a marginal effect on neuropathy following overexpression of normal or mutant tau protein in flies. Functional analogues of the large non-coding hsromega transcripts in human thus appear to be promising candidates as therapeutic targets for the polyQ-mediated neurodegenerative diseases.

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Section: Non-coding Hsrω Rna and Polyq Toxicity In Drosophilamentioning

confidence: 99%

RNAi for the large non-coding hsrω transcripts suppresses polyglutamine pathogenesis inDrosophilamodels

Mallik

Lakhotia²

2009

RNA Biology

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108

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“…For example, Dic61B codes for an axonemal dynein intermediate chain exhibiting strong testis-biased expression (FlyBase); it is required for development and precise assembly of sperm axonemes and is essential for male fertility in D . melanogaster [87,88]. Given the rapid evolution of sperm length in D .…”

Section: Resultsmentioning

confidence: 99%

Genomics of parallel adaptation at two timescales in Drosophila

Zhao

Begun

2017

PLoS Genet

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Two interesting unanswered questions are the extent to which both the broad patterns and genetic details of adaptive divergence are repeatable across species, and the timescales over which parallel adaptation may be observed. Drosophila melanogaster is a key model system for population and evolutionary genomics. Findings from genetics and genomics suggest that recent adaptation to latitudinal environmental variation (on the timescale of hundreds or thousands of years) associated with Out-of-Africa colonization plays an important role in maintaining biological variation in the species. Additionally, studies of interspecific differences between D. melanogaster and its sister species D. simulans have revealed that a substantial proportion of proteins and amino acid residues exhibit adaptive divergence on a roughly few million years long timescale. Here we use population genomic approaches to attack the problem of parallelism between D. melanogaster and a highly diverged conger, D. hydei, on two timescales. D. hydei, a member of the repleta group of Drosophila, is similar to D. melanogaster, in that it too appears to be a recently cosmopolitan species and recent colonizer of high latitude environments. We observed parallelism both for genes exhibiting latitudinal allele frequency differentiation within species and for genes exhibiting recurrent adaptive protein divergence between species. Greater parallelism was observed for long-term adaptive protein evolution and this parallelism includes not only the specific genes/proteins that exhibit adaptive evolution, but extends even to the magnitudes of the selective effects on interspecific protein differences. Thus, despite the roughly 50 million years of time separating D. melanogaster and D. hydei, and despite their considerably divergent biology, they exhibit substantial parallelism, suggesting the existence of a fundamental predictability of adaptive evolution in the genus.

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“…The recessive male sterile ms 21 mutation was maintained with the TM6B balancer [7], which established its linkage to chromosome 3 [6]. To map the cytological location of the ms 21 mutation, a series of overlapping, molecularly defined Drosdel and Exelixis deficiencies spanning the entire third chromosome were used.…”

Section: Resultsmentioning

confidence: 99%

“…However, further studies on the hsrω 05241 allele revealed that the recessive male sterility, initially ascribed to the mutation in hsrω gene, was actually due to a background second site mutation, which was named as ms 21 [6].…”

Section: Introductionmentioning

confidence: 99%

Drosophila Dynein Intermediate Chain Gene, Dic61B, Is Required for Spermatogenesis

Fatima

2011

PLoS ONE

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This study reports the identification and characterization of a novel gene, Dic61B, required for male fertility in Drosophila. Complementation mapping of a novel male sterile mutation, ms21, isolated in our lab revealed it to be allelic to CG7051 at 61B1 cytogenetic region, since two piggyBac insertion alleles, CG7051c05439 and CG7051f07138 failed to complement. CG7051 putatively encodes a Dynein intermediate chain. All three mutants, ms21, CG7051c05439 and CG7051f07138, exhibited absolute recessive male sterility with abnormally coiled sperm axonemes causing faulty sperm individualization as revealed by Phalloidin staining in Don Juan-GFP background. Sequencing of PCR amplicons uncovered two point mutations in ms21 allele and confirmed the piggyBac insertions in CG7051c05439 and CG7051f07138 alleles to be in 5′UTR and 4th exon of CG7051 respectively, excision of which reverted the male sterility. In situ hybridization to polytene chromosomes demonstrated CG7051 to be a single copy gene. RT-PCR of testis RNA revealed defective splicing of the CG7051 transcripts in mutants. Interestingly, expression of cytoplasmic dynein intermediate chain, α, β, γ tubulins and α-spectrin was normal in mutants while ultra structural studies revealed defects in the assembly of sperm axonemes. Bioinformatics further highlighted the homology of CG7051 to axonemal dynein intermediate chain of various organisms, including DNAI1 of humans, mutations in which lead to male sterility due to immotile sperms. Based on these observations we conclude that CG7051 encodes a novel axonemal dynein intermediate chain essential for male fertility in Drosophila and rename it as Dic61B. This is the first axonemal Dic gene of Drosophila to be characterized at molecular level and shown to be required for spermatogenesis.

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The hsrω 05241 allele of the noncoding hsrω gene of Drosophila melanogaster is not responsible for male sterility as reported earlier

Abstract: The hsrω 05241 allele of the noncoding hsrω gene of Drosophila melanogaster is not responsible for male sterility as reported earlier

Cited by 6 publications

References 7 publications

RNAi for the large non-coding hsrω transcripts suppresses polyglutamine pathogenesis inDrosophilamodels

RNAi for the large non-coding hsrω transcripts suppresses polyglutamine pathogenesis inDrosophilamodels

Genomics of parallel adaptation at two timescales in Drosophila

Drosophila Dynein Intermediate Chain Gene, Dic61B, Is Required for Spermatogenesis

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