The human embryo following biopsy on day 5 versus day 3: viability, ultrastructure and spindle/chromosome configurations

Chatzimeletiou, Katerina; Petrogiannis, N; Sioga, Antonia; Emmanouil-Nikoloussi, Elpida-Niki; Panagiotidis, Yannis; Prapa, Marialena; Patrikiou, Antonios; Filippa, Maria; Zervakakou, Glykeria; Papanikolaou, Kyriakos; Makedos, Anastasios; Kolibianakis, Efstratios; Tarlatzis, Basil C.; Grimbizis, Grigoris

doi:10.1016/j.rbmo.2022.02.022

Cited by 4 publications

(5 citation statements)

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“…However, embryonic development and subsequent implantation may be impaired in embryos derived from sperm with fragmented DNA. Although oocytes have the machinery to repair DNA damage, factors such as the quality of the oocyte itself and the type of sperm DNA damage may influence the extent to which this repair occurs [ 40 , 41 , 42 ]. Therefore, it is of utmost importance to accurately diagnose DNA fragmentation in sperm and, in men with high levels of sperm DNA fragmentation, suggest suitable treatments before the initiation of their assisted reproduction cycle.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry

Chatzimeletiou,

Fleva,

Nikolopoulos

et al. 2023

Medicina

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Background and Objectives: Sperm DNA fragmentation refers to any break in one or both of the strands of DNA in the head of a sperm. The most widely used methodologies for assessing sperm DNA fragmentation are the sperm chromatin structure assay (SCSA), the sperm chromatin dispersion assay (SCD), the single-cell gel electrophoresis assay (SCGE–comet), and the terminal-deoxynucleotidyl-transferase (TdT)-mediated dUTP nick end labelling (TUNEL) assay. The aim of this study was to compare the efficiency and sensitivity of the analysis of sperm DNA fragmentation using TUNEL via fluorescence microscopy, and flow cytometry. Materials and Methods: Semen samples were collected and analyzed for standard characteristics using light microscopy, and for sperm DNA fragmentation using both TUNEL via fluorescence microscopy, and flow cytometry. Results: There were no significant differences in the values of the sperm DNA fragmentation index (DFI) obtained when the analysis was performed using TUNEL or flow cytometry (p = 0.543). Spearman’s correlation analysis revealed a significant negative correlation between sperm motility (%) and sperm DNA fragmentation (p < 0.01), as well as between sperm concentration and sperm DNA fragmentation (p < 0.05). The Mann–Whitney U test showed no significant difference in the DFI among couples with repeated implantation failure (RIF) and miscarriages (p = 0.352). Conclusions: Both methods (TUNEL via fluorescence microscopy, and flow cytometry) have a high efficiency and sensitivity in accurately detecting sperm DNA fragmentation, and can be effectively used to assess male fertility.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry

Chatzimeletiou,

Fleva,

Nikolopoulos

et al. 2023

Medicina

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“…More specifically, among the numerical chromosomal abnormalities diagnosed by PGT is trisomy 21 (Down syndrome), contributing to the ongoing discussion regarding discrimination against people born with the particular syndrome and the potential use of PGT as a eugenics tool, as well as presenting future parents with the dilemma to proceed with the implantation of an aneuploid embryo or discard it. Moreover, the accuracy of the biopsy itself to properly determine embryo ploidy has been challenged, since studies showed that mosaicism exists at all stages of preimplantation development, due to the lack of cell cycle check pointsleading to spindle and nuclear abnormalities [ 4 , 5 , 14 , 42 , 43 ]. It is important to note that low-medium mosaicism in the trophectoderm mostly arises after TE and ICM differentiation, and such embryos have equivalent developmental potential as fully euploid ones [ 44 , 45 , 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Preimplantation genetic testing for aneuploidy (PGT-A) has therefore been proposed as a solution that allows the diagnosis of chromosomal abnormalities in embryos before implantation to the uterus [ 10 , 11 ]. PGT-A is offered to patients of advanced maternal age (AMA) ≥ 35 years, repeated implantation failure (RIF) > 3, repeated miscarriages (RM) > 2, previous pregnancy with a chromosomally abnormal fetus irrespective of maternal age, and in cases of severe male factor infertility including non-obstructive azoospermia (NOA) and oligoasthenoteratozoospermia (OAT) [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…The most widely used embryo biopsy strategies for PGT are: (a) cleavage stage biopsy on day 3 followed by aspiration of a single blastomere and fresh embryo transfer on day 5, and (b) blastocyst stage biopsy on day 5 followed by aspiration of 5–10 trophectoderm (TE) cells, immediate vitrification of the blastocyst post biopsy, and transfer after warming at a future cycle, in a synchronized well-prepared endometrium [ 13 , 14 , 16 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Clinical Outcome, Socioeconomic Status and Psychological Constrains of Patients Undergoing Preimplantation Genetic Testing (PGT) in Northern Greece

et al. 2022

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Background and objectives: Preimplantation genetic testing (PGT) offers patients the possibility of having a healthy baby free of chromosomal or genetic disorders. The present study focuses on the application of PGT for patients located in Northern Greece, investigating their clinical outcomes, their motives, and their overall physical and emotional experience during the treatment, in association with their socioeconomic background. Materials and Methods: Couples who underwent PGT for a monogenic condition (PGT-M, n = 19 cycles) or aneuploidy (PGT-A, n = 22 cycles) participated in the study. Fertilization, implantation, and pregnancy rates were recorded for all cycles. The couples were asked to fill in a questionnaire about the consultation they had received prior to treatment, their sociodemographic information, and the psychological impact PGT had on both the female and male partner. Results: The fertilization, implantation, and ongoing pregnancy rates for the PGT-M and PGT-A cycles were 81.3%, 70.6%, and 52.9%, and 78.2%, 64.3%, and 57.1%, respectively. Females experienced more intense physical pain than their male partners while psychological pain was encountered by both partners and occasionally in higher instances in males. No typical socioeconomic background of the patients referred for PGT in Northern Greece was noticed. Conclusion: PGT is an attractive alternative to prenatal diagnosis (PND), aiming to establisha healthy pregnancy by identifying and avoiding the transfer of chromosomally or genetically abnormal embryos to the uterus. Although the benefits of PGT were well-received by all patients undergoing the procedure, psychological pain was evident and especially prominent in patients with a previous affected child or no normal embryos for transfer. Holistic counseling is of utmost importance in order to make patients’ experience during their journey to have a healthy baby less emotionally demanding and help them make the right choices for the future.

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“…Cryopreservation of human embryos significantly improved in the last decade with the introduction of vitrification protocols (8)(9)(10). Embryo vitrification has been routinely applied for the "freeze-all" (FA) strategy, based on ovarian stimulation segmentation, the ovulation triggering, vitrified-warmed embryo transfer in subsequent natural or artificial cycles, and all viable embryos' elective cryopreservation (11).…”

Section: Introductionmentioning

confidence: 99%

The pregnancy results were not affected from the administration day of Depot GnRH agonists in artificial cycle frozen-thawed embryo transfers

AĞAR¹,

MADENLİ>

Güngör

et al. 2023

Journal of Medicine and Palliative Care

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Aim: In frozen-thawed embryo transfers (FET), Gonadotropin-Releasing Hormone (GnRH) agonists have recently been used to improve implantation results. It is preferred to administer it in the luteal phase of the previous cycle. The objective was to compare the effects of different administration days of depot GnRH agonists on implantation and pregnancy rates in the artificial cycle of FET. Material and Method: A retrospective case-control study was conducted in an in vitro fertilization (IVF) center in a university hospital, including all women starting an artificial cycle of FET. One thousand two hundred and twenty-seven (n:1227) FET cycles were scanned from the files from October 2014 to December 2021. Depot agonists (Lucrin depot 3.75 mg sc Abbott USA.-leuprolide acetate) were used in 219 patients with endometriosis. In 58 patients, it was administered on day 21 of the previous cycle (Group 1), and in 161 patients, it was administered on day 2 of the same cycle (Group 2). Results: This study showed no statistically significant difference between the two groups in laboratory parameters and endometrial thickness (p>0.05). There was no statistically significant association between the abort rate and transfer day (p>0.05). There was no statistically significant association between the pregnancy results and transfer day (p>0.05). The ongoing pregnancy rate (OPR) rate was relatively high in the second group compared to the twenty-first day of the previous cycle (87/161(54%) vs. 30/58 (51.7%)). The biochemical pregnancy was relatively high in the second-day group compared to the twenty-first day of the previous cycle (62/161(38.5%) vs. 21/58 (36.2%)). The abort rate was relatively high in the twenty-first-day group compared to the second day of the cycle (25/87(28.75%) vs. 9/30(30%)). Conclusion: In conclusion, the impacts of various administration days of depot Gonadotropin-releasing hormone (GnRH) agonists on implantation and pregnancy rates were not statistically significant.

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The human embryo following biopsy on day 5 versus day 3: viability, ultrastructure and spindle/chromosome configurations

Cited by 4 publications

References 70 publications

Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry

Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry

Clinical Outcome, Socioeconomic Status and Psychological Constrains of Patients Undergoing Preimplantation Genetic Testing (PGT) in Northern Greece

The pregnancy results were not affected from the administration day of Depot GnRH agonists in artificial cycle frozen-thawed embryo transfers

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