The human female reproductive tract: Immunohistological localization of γA, γG, γM, secretory “piece,” and lactoferrin

Tourville, D.R.; Ogra, S.S.; Lippes, Jack; Tomasi, Thomas B.

doi:10.1016/0002-9378(70)90460-6

Cited by 139 publications

(58 citation statements)

References 21 publications

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“…Few human tissues (haematopoietic cells aside) are convincingly positive, with reports of lactoferrin in secretory phase endometrium (Tourville et al, 1970) and in seminal plasma (Goodman & Young, 1981) as a major sperm-coating antigen (Heckman & Rumke, 1969). Immunocytochemical staining has been reported (Mason & Taylor, 1978) in gastric mucus neck cells, some duodenal absorptive epithelia, glandular bronchial glands and most strongly in glandular epithelia (and their secretions) of lactating breast; also in the odd sample of uterus and a basal cell skin carcinoma.…”

Section: Discussionmentioning

confidence: 99%

Isolation of a lactoferrin cDNA clone and its expression in human breast cancer

Campbell

Skilton²,

Coombes³

et al. 1992

Br J Cancer

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Summary A cDNA library constructed from mRNA from a human breast carcinoma metastasis was screened with a polyclonal antibody to deglycosylated human milk fat globule membrane, resulting in the isolation of eight clones from a total of 105 plaques. One of these (J16) was identified as lactoferrin. It was highly expressed (as a 2.5 Kb mRNA) in lactating breast and in both normal resting tissue taken from adjacent to carcinoma or from reduction mammoplasties. Immunoreactive lactoferrin was localised to ductal cells and their secretions in both normal and mildly hyperplastic ducts. In a normal tissue screen J16 was highly expressed in stomach, poorly in skin and lymphocytes and absent from other organs examined. It was variably expressed in 33/59 invasive primary breast tumours; lactoferrin protein in these was heterogeneously distributed in epithelial tumour foci. Presence of J16 was inversely related to expression of oestrogen receptor protein (P = 0.0001). There was no significant relationship to other clinical parameters. We also found immunoreactivity in 20/41 (49%) cases of ductal carcinoma in situ. Expression was not observed in any breast or gastric cell line examined. Thus lactoferrin appears to be down regulated in some forms of cancer. The presence of lactoferrin could be a contraindication for effective endocrine therapy.

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Section: Discussionmentioning

confidence: 99%

Isolation of a lactoferrin cDNA clone and its expression in human breast cancer

Campbell

Skilton²,

Coombes³

et al. 1992

Br J Cancer

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“…Secretory component is manufactured in secretory epithelial cells (MartinezTello et al 1968;O'Daly and Cebra 1968;Tourville et al 1970) and is bound to dimeric IgA by covalent and disulfide bonds during passage of the immunoglobulin across the epithelial cell (Heremans and Vaerman 1971). There have been numer()us suggestions that secretory component serves a receptor and transport function in collecting IgA and ferrying it from the basal membrane of the epithelial cell to the lumen.…”

Section: Secretion Of Locally Synthesized Antibodymentioning

confidence: 99%

Immunological Functions of the Mammary Gland and Its Secretion-Comparative Review

Dl¹

1980

Aust. Jnl. Of Bio. Sci.

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The mammary gland performs vitally important immunological roles, both in providing passive immune protection to the suckling infant and in immunological defence of its own tissues against infection with microorganisms. These immunological functions differ greatly between species of mammals in both nature and magnitude. In ungulates the mammary gland is singularly responsible for transfer of immunoglobulin (IgG) from mother to young. This process is dependent on a highly selective mechanism which results in the transport of blood-borne IgG molecules across secretory epithelial cells of the colostrum-forming mammary gland and into secretion. Upon ingestion of colostrum by the young ungulate this immunoglobulin is absorbed across the wall of the small intestine and thence into the bloodstream.In other species, including rodents and primates, there is a well-developed local IgA system operating in the mammary gland. In this situation, plasma cells located near the basal membranes of secretory epithelial cells secrete IgA which passes through the epithelial cells and into colostrum or milk. In these species the IgA in mammary secretions is not absorbed into the circulation of the suckling infant; because of its unique property of resisting proteolytic degradation, it may mediate a local protective role in the lumen of the intestine of the suckling infant.Specific immunological protection of mammary tissue may be mediated through blood-derived antibody (particularly IgG), locally synthesized antibody (particularly IgA) or phagocytic cells. Neutrophils arrive in mammary tissue and secretions in very large numbers following bacterial invasion of the gland. It has been established recently that these cells carry cytophilic antibody on their cell membrane. This cytophilic antibody can play an important functional role in enhancing the phagocytic capacity of neutrophils in the mammary gland.

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“…Lactoferrin, an iron-binding glycoprotein originally discovered in bovine whey, is also present in human milk, wet surface mucosa, tears, and saliva (194,195), and secondary granules of mature neutrophils (196). Lactoferrin is also found in organs of reproduction such as mammary glands (197) and the uterus (198,199). The known biological functions of lactoferrin are the stimulation of DNA synthesis (200), the transport of iron through the fetal intestine (201), the modulation of the immune system (202,203), and bacteriostatic/bactericidal activities by chelating iron (204).…”

Section: Altered Steroid Hormone Homeostasis In Fishmentioning

confidence: 99%

Chronic Toxicity of Environmental Contaminants: Sentinels and Biomarkers

LeBlanc¹,

Bain²

1997

Environmental Health Perspectives

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Due to the use of a limited number of species and subchronic exposures, current ecological hazard assessment processes can underestimate the chronic toxicity of environmental contaminants resulting in adverse responses of sentinel species. Several incidences where sentinel species have responded to the effects of chronic exposure to ambient levels of environmental contaminants are discussed, including the development of neoplasia in fish, immunosuppression in marine mammals, pseudohermaphrodism in invertebrates, teratogenicity in amphibians, and aberrations in the sexual development of fish and reptiles. Biomarkers of chronic toxicity, including DNA mutations, alterations in specific protein and mRNA levels, and perturbations in metabolism, are presented. The incorporation of appropriate surrogate species and biomarkers of chronic toxicity into standard toxicity characterizations is proposed as a means of significantly refining the ecological hazard assessment process. -Environ Health Perspect 105(Suppl 1): 65-80 (1997)

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The human female reproductive tract: Immunohistological localization of γA, γG, γM, secretory “piece,” and lactoferrin

Cited by 139 publications

References 21 publications

Isolation of a lactoferrin cDNA clone and its expression in human breast cancer

Isolation of a lactoferrin cDNA clone and its expression in human breast cancer

Immunological Functions of the Mammary Gland and Its Secretion-Comparative Review

Chronic Toxicity of Environmental Contaminants: Sentinels and Biomarkers

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