The Human Host Defense Ribonucleases 1, 3 and 7 Are Elevated in Patients with Sepsis after Major Surgery—A Pilot Study

Abstract

Sepsis is the most common cause of death in intensive care units and associated with widespread activation of host innate immunity responses. Ribonucleases (RNases) are important components of the innate immune system, however the role of RNases in sepsis has not been investigated. We evaluated serum levels of RNase 1, 3 and 7 in 20 surgical sepsis patients (Sepsis), nine surgical patients (Surgery) and 10 healthy controls (Healthy). RNase 1 and 3 were elevated in Sepsis compared to Surgery (2.2- and 3.1-fold, respectively; both p < 0.0001) or compared to Healthy (3.0- and 15.5-fold, respectively; both p < 0.0001). RNase 1 showed a high predictive value for the development of more than two organ failures (AUC 0.82, p = 0.01). Patients with renal dysfunction revealed higher RNase 1 levels than without renal dysfunction (p = 0.03). RNase 1 and 3 were higher in respiratory failure than without respiratory failure (p < 0.0001 and p = 0.02, respectively). RNase 7 was not detected in Healthy patients and only in two patients of Surgery, however RNase 7 was detected in 10 of 20 Sepsis patients. RNase 7 was higher in renal or metabolic failure than without failure (p = 0.04 and p = 0.02, respectively). In conclusion, RNase 1, 3 and 7 are secreted into serum under conditions with tissue injury, such as major surgery or sepsis. Thus, RNases might serve as laboratory parameters to diagnose and monitor organ failure in sepsis.

Ribonuclease 1 attenuates septic cardiomyopathy and cardiac apoptosis in a murine model of polymicrobial sepsis

“…Specifically, we report for the first time to our knowledge that the levels of RNH1, the endogenous inhibitor of RNase 1, are significantly elevated on the day of diagnosis and 3 days after diagnosis of sepsis ( Figure 1A). We have also recently reported an increased serum expression of RNase 1 at the onset of sepsis (22). RNH1 specifically binds to RNase 1 and inhibits its activity (20).…”

“…RNH1 is elevated in serum of septic patients. We recently reported that serum levels of RNase 1 are substantially elevated in the early phase of sepsis (22). Because RNH1 inhibits the enzymatic activity of RNase 1, serum levels of RNH1 in septic patients (n = 21) were investigated on the day of and 3 days after diagnosis.…”

“…Serum sampling. After 10 minutes of coagulation at room temperature, the serum samples were centrifuged at 2000 g for 10 minutes and stored at -80°C (22). The characteristics of the study population are shown in Supplemental Table 1 according to the groups: healthy subjects, patient with sepsis on the day of diagnosis (Sepsi DD), and patients with sepsis 3 days after diagnosis (Sepsis D3).…”

Potential humoral mediators of remote ischemic preconditioning in patients undergoing surgical coronary revascularization

“…For the detection of Apo-A1 90 , EPO 91 , GDF-11 92 , RNase-1 93 (ELISA Cloud-Immunoassay, Houston, USA) and HIF-1α 94 (RayBio, Georgia, USA) avidin-conjugated horseradish peroxidase was supplemented. For the detection of GLP-1 95 (Abcam, Cambridge, UK) an antibody cocktail consisting of a capture and a detector antibody was supplemented.…”

Antifungal Activity of Human and Some Mammals Sera

“…Наиболее представленными в человеческой сыворотке в количественном отношении являются лизоцим (2800±800 нг/мл) [7], РНКазы (2200±400 нг/мл) [10] и дермцидины (2100±100 нг/мл) [11]. Их концентрации в данном ликворе вполне соотносятся с их минимальными ингибирующими концентрациями в отношении C. albicans, что дает основание считать именно эти АМП отвечающими за противокандидозную активность сыворотки.…”