The human LFA-3 gene is located at the same chromosome band as the gene for its receptor CD2

Abstract

Lymphocyte function-associated antigen 3 (LFA-3) is a widely distributed cell surface glycoprotein that has been assigned a role in cell-cell adhesion on the basis of its capacity to bind to the T-lymphocyte CD2 antigen. The amino acid sequences of the extracellular domains of these two antigens, predicted from their cDNA sequences, show significant similarities, and both are members of the immunoglobulin supergene family. In this communication, a probe prepared from LFA-3 cDNA has been used in Southern blot analyses of somatic cell hybrids and in in situ hybridization to assign the LFA-3 gene to the human chromosome band 1p13. This is the same location previously assigned to CD2. Thus the LFA-3 and CD2 genes have probably arisen by duplication of a common evolutionary precursor. These genes therefore represent a further instance in which related members of the immunoglobulin superfamily are located in adjacent regions of the genome.

Molecular Cloning of a Human cDNA IGSF3 Encoding an Immunoglobulin-like Membrane Protein: Expression and Mapping to Chromosome Band 1p13

“…The apparent clustering of these related genes raises the possibility that they have been generated by duplication of an ancestral IgSF gene. Other immune cell receptors genes, CD2 and its counter receptor CD58, are also localized in this region (Sewell et al, 1988), in addition to the translocation breakpoint (t(1;22)(p13;q13)) associated with acute megakaryocytic leukemia (Lion et al, 1992).…”

Ligand-induced Conformational Change Within the CD2 Ectodomain Accompanies Receptor Clustering: Implication for Molecular Lattice Formation

“…Additionally, it has been shown that CD2 binds to a second structurally related ligand, human CD48, but with an affinity two orders of magnitude weaker (K d 0 10 −4 M) than that of the CD2-CD58 interaction (K d 0 10 −6 M; Arulanandam et al, 1993a). The CD2, CD58 and CD48 molecules are each thought to have evolved from a common homophilic interaction precursor (Staunton et al, 1987;Sewell et al, 1988;Wong et al, 1990). In the rodent species, CD48 is the only known ligand for CD2 (Kato et al, 1992).…”

Gene structure, chromosomal location, and basis for alternative mRNA splicing of the human VCAM1 gene.

“…Ofthe 60 metaphases, 25 (41.7%) had a signal at 1p31-32. We note that two structurally homologous members of the immunoglobulin supergene family, lymphocyte function-associated antigen (LFA-3) and CD2 are also found on the short arm of chromosome 1 (31,32). Interestingly, a locus at 1p32, designated T-cell lymphoma 5 (TCLS), may specify a DNA binding protein with a helix-loop-helix domain (33,34).…”