The human nasal mucosa in the menstrual cycle

Toppozada, Hussein H.; Michaels, L.; Toppozada, M.; El-Ghazzawi, E.; Talaat, Ahmed; Elwany, Samy

doi:10.1017/s0022215100092082

Cited by 35 publications

(12 citation statements)

References 13 publications

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“…The relationship between the hormone estrogen and the nasal mucosa has been postulated by clinical studies that advocate the existence of an entity named hormonal rhinitis, or nasal diseases caused by variations in the hormone levels, especially estrogen, and according to the work of Philpott et al (2004Philpott et al ( , 2008, Shirasaki et al (2004); Toppozada et al (1981Toppozada et al ( , 1982Toppozada et al ( , 1984Toppozada et al ( , 1988 and Zhao et al (2001).…”

Section: Discussionmentioning

confidence: 99%

“…Estrogen receptors (ER) are detectable in the human body tissues, including tissues of reproductive organs, nasal mucosa and nervous system (Ciocca & Roig, 1995;Bowser & Riederer, 2001;Berger et al, 2003;Kuiper et al, 1997;Millas et al, 2011;Philpott et al, 2004Philpott et al, , 2008Shirasaki et al, 2004;Toppozada et al, 1981Toppozada et al, , 1982Toppozada et al, , 1984Toppozada et al, , 1988. To evaluate staining intensity, different methods have been designed as RT-PCR and immunohistochemistry (Bowser & Riederer;Pavao & Traish;Shirasaki et al;Zhao et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods

et al. 2014

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SUMMARY:Immunohistochemistry is a specific and sensitive staining method for detection of several proteins. One important function of this method is to help us on the diagnosis of the presence of specific receptors as the estrogen receptors. The aim of this study was to compare two different methods to evaluate immunohistochemical staining intensity to detect the presence of ER in the nasal mucosa tissue: one using a digital system and the other through conventional direct microscopy observation. Sixty two stained samples were observed and analyzed under optic microscopy by three specialized professionals, who have graded intensity from: absent, mild, moderate and intense staining. Afterwards, an objective measurement was obtained by a relative optical density (ROD) reading, through the MCID 7.0 system of densitometric digital analysis (Inter Focus Imaging LTDA, Linton, England). Subjective and objective classifications were compared under statistical analysis (Kolmogorov-Smirnov and Spearman Correlation Test, p<0.05). We found a positive correlation between the subjective findings of observers and digital analysis in all the categories of beta receptor. For the alpha receptor, there was a correlation only in extreme categories. The subjective evaluations by observers and digital method by measuring the relative optical density show statistically significant correlations in quantifying the estrogen receptors by immunohistochemistry staining. This indicates that both methods show accuracy for the proposed study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods

et al. 2014

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“…Such patterns are generally considered typical of cells producing and discharging the mucous secretion in a continuous, active and energetic mode, as under flogistic conditions (Jahnke 1972;Toppozada et al 1981;Rucci et al 1984). This can induce variations in the glycoproteic composition of the mucus, which suggested that the term "seromucous secretion" could be more correct (Casasco 1989).…”

Section: Discussionmentioning

confidence: 99%

In vitro effects of some differentiation inductors in metaplastic epithelium of the human nasal cavity

Gulisano

Pacini

Ruggiero

et al. 1996

Cell and Tissue Research

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The alterations in the mucociliary unit in the course of chronic inflammation of the upper respiratory tract correspond to morphologic anomalies of the respiratory epithelium and induce cuboidal and squamous metaplasia. While the squamous pattern is most probably irreversible, it is still not clear whether it is possible to restore ciliogenesis in cuboidal metaplasia. In the present study, the action of different inductors of differentiation was evaluated in vitro in isolated cells and explants from human nasal metaplastic epithelium. Polar/apolar compounds induced secretory activity, whereas retinoic acid was able to induce ciliogenesis in some cases. Therefore, the cuboidal metaplastic condition appears to be reversible, and two distinct pathways of differentiation, secretory and ciliogenetic, are identifiable.

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“…Helmi et al [19] and Toppozada et al [20] reviewed the effects of sex hormones on the nasal mucosa and reported squamous metaplasia, glandular hyperactivity and vas cular congestion following prolonged admin istration of contraceptive pills and estrogens. None of these features was observed in the present series thus invalidating the hor monal theory [21], On the other hand, the correctness of the older telelogic speculation of estrogen depletion has been questioned in Harrison's [21] review article, as it was hard to explain the absence of signs of estrogen deficiency in other more estrogen-dependent organs.…”

Section: Discussionmentioning

confidence: 99%