The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogaster

Frey, Th.; Kwadha, Charles A.; Haag, Franziska; Pelletier, Julien; Wallin, Erika A.; Holgersson, Elsa; Hedenström, Erik; Bohman, Björn; Bengtsson, Marie; Becher, Paul G.; Krautwurst, Dietmar; Witzgall, Peter

doi:10.1016/j.isci.2022.105269

Cited by 3 publications

(2 citation statements)

References 111 publications

(153 reference statements)

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“…Evidence of limited ab3A spiking when we attempted to express GCPRs into ab3A neurons (Figure 1) directed our efforts towards selecting only the most active ligands for these ORs, based on studies reported for OR1A1 (Geithe et al, 2017) and OR2W1 (Adipietro et al, 2012), indicating both the isomers of carvone as the most promising (Figure 2). In addition, we included several aldehydes in our screening, among which (Z)-4-nonenal, (Z)-4-undecenal, and (Z)-6-undecenal have been more recently demonstrated to be the strongest candidates to activate OR2W1 (Frey et al, 2022). While the given absence of any effect may indicate the basic ab3A spiking to be an artifact (Figure 2), we cannot exclude that such spiking may result from the expression of a metabotropically regulating subunit involved in gating the wide asset of ion channels that are expressed in Drosophila neurons (Hodge, 2009).…”

Section: Discussionmentioning

confidence: 99%

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Heterologous investigation of metabotropic and ionotropic odorant receptors in ab3A neurons of Drosophila melanogaster

Pettersson,

Cattaneo

2024

Front. Mol. Biosci.

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In insects, antennal ionotropic receptors (IRs) and odorant receptors (ORs) are among the main sensors of olfactory cues. To functionally characterize the subunits from these receptors, the use of ab3A neurons from transgenic Drosophila melanogaster represented one of the most powerful tools, allowing the identification of ligands (deorphanization) and decrypting their pharmacological properties. However, further investigation is needed to shed light on possible metabotropic functionalities behind insect olfactory receptors and test potentials from the up-to-now-used empty neuronal systems to express subunits belonging to variegate receptor classes. In this project, we adopted the most updated system of Drosophila ab3A empty neurons to test various olfactory receptors, ranging from human ORs working as metabotropic G-protein coupled receptors to insect ionotropic IRs and ORs. Testing transgenic Drosophila expressing human ORs into ab3A neurons by single sensillum recording did not result in an OR response to ligands, but it rather re-established neuronal spiking from the empty neurons. When transgenic D. melanogaster expressed ionotropic IRs and ORs, both heterologous and cis-expressed IRs were non-functional, but the Drosophila suzukii OR19A1 subunit responded to a wide asset of ligands, distinguishing phasic or tonic compound-dependent effects. Despite the use of Drosophila ab3A neurons to test the activation of some metabotropic and ionotropic receptor subunits resulted non-functional, this study deorphanized a key OR of D. suzukii demonstrating its binding to alcohols, ketones, terpenes, and esters.

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Section: Discussionmentioning

confidence: 99%

“…Compounds tested on human ORs were selected from previous studies demonstrating tuning of OR1A1 and OR2W1 (Adipietro et al, 2012;Geithe et al, 2017) and adding a panel of aldehydes from our previous screening (Cattaneo et al, 2022) that have also been reported to be active on the human receptors (Frey et al, 2022).…”

Section: Stimulimentioning

confidence: 99%

Heterologous investigation of metabotropic and ionotropic odorant receptors in ab3A neurons of Drosophila melanogaster

Pettersson,

Cattaneo

2024

Front. Mol. Biosci.

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M2OR: a database of olfactory receptor–odorant pairs for understanding the molecular mechanisms of olfaction

Lalis,

Hladiš,

Khalil

et al. 2023

Nucleic Acids Research

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Mammalian sense of smell is triggered by interaction between odorant molecules and a class of proteins, called olfactory receptors (ORs). These receptors, expressed at the surface of olfactory sensory neurons, encode myriad of distinct odors via a sophisticated activation pattern. However, determining the molecular recognition spectrum of ORs remains a major challenge. The Molecule to Olfactory Receptor database (M2OR, https://m2or.chemsensim.fr/) provides curated data that allows an easy exploration of the current state of the research on OR-molecule interaction. We have gathered a database of 75,050 bioassay experiments for 51 395 distinct OR-molecule pairs. Drawn from published literature and public databases, M2OR contains information about OR responses to molecules and their mixtures, receptor sequences and experimental details. Users can obtain information on the activity of a chosen molecule or a group of molecules, or search for agonists for a specific OR or a group of ORs. Advanced search allows for fine-grained queries using various metadata such as species or experimental assay system, and the database can be queried by multiple inputs via a batch search. Finally, for a given search query, users can access and download a curated aggregation of the experimental data into a binarized combinatorial code of olfaction.

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Sex Pheromone Mediates Resource Partitioning Between Drosophila melanogaster and D. suzukii

Kwadha,

Rehermann,

Tasso

et al. 2024

Evolutionary Applications

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The spotted‐wing drosophila, Drosophila suzukii and the cosmopolitan vinegar fly D. melanogaster feed on soft fruit and berries and widely overlap in geographic range. The presence of D. melanogaster reduces egg‐laying in D. suzukii, possibly because D. melanogaster outcompetes D. suzukii larvae feeding in the same fruit substrate. Flies use pheromones to communicate for mating, but pheromones also serve a role in reproductive isolation between related species. We asked whether a D. melanogaster pheromone also modulates oviposition behaviour in D. suzukii. A dual‐choice oviposition assay confirms that D. suzukii lays fewer eggs on blueberries exposed to D. melanogaster flies and further shows that female flies have a stronger effect than male flies. This was corroborated by treating berries with synthetic pheromones. Avoidance of D. suzukii oviposition is mediated by the female D. melanogaster pheromone (Z)‐4‐undecenal (Z4‐11Al). Significantly fewer eggs were laid on berries treated with synthetic Z4‐11Al. In comparison, the male pheromone (Z)‐11‐octadecenyl acetate (cVA) had no effect on D. suzukii oviposition. Z4‐11Al is a highly volatile compound that is perceived via olfaction and it is accordingly behaviourally active at a distance from the source. D. suzukii is known to engage in mutual niche construction with the yeast Hanseniaspora uvarum, which strongly attracts flies. Adding Z4‐11Al to fermenting H. uvarum significantly decreased D. suzukii flight attraction in a laboratory wind tunnel and a field trapping assay. That a D. melanogaster pheromone regulates oviposition in D. suzukii demonstrates that heterospecific pheromone communication contributes to reproductive isolation and resource partitioning in cognate species. Stimulo‐deterrent diversion or push‐pull methods, building on combined use of attractant and deterrent compounds, have shown promise for control of D. suzukii. A pheromone that specifically reduces D. suzukii attraction and oviposition adds to the toolbox for D. suzukii integrated management.

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The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogaster

Cited by 3 publications

References 111 publications

Heterologous investigation of metabotropic and ionotropic odorant receptors in ab3A neurons of Drosophila melanogaster

Heterologous investigation of metabotropic and ionotropic odorant receptors in ab3A neurons of Drosophila melanogaster

M2OR: a database of olfactory receptor–odorant pairs for understanding the molecular mechanisms of olfaction

Sex Pheromone Mediates Resource Partitioning Between Drosophila melanogaster and D. suzukii

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