The Human Spindle Assembly Checkpoint Protein Bub3 Is Required for the Establishment of Efficient Kinetochore–Microtubule Attachments

Logarinho, Elsa; Resende, Tatiana P.; Torres, C.E. Rodrı́guez; Bousbaa, Hassan

doi:10.1091/mbc.e07-07-0633

Cited by 93 publications

(127 citation statements)

References 65 publications

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“…Depletion of Bub3 produced a severe defect in nocodazole-induced, chronic mitotic checkpoint signaling ( Fig. S1 A and B), as expected from prior work (23). The BubR1 E409K variant with a defect in Bub3 binding gave rise to extensive chromosome misalignment yet failed to delay anaphase onset in an unperturbed mitosis ( Fig.…”

Section: Resultssupporting

confidence: 81%

“…Bub3 was identified as a mitotic checkpoint protein through genetic screening in budding yeast (19), and subsequent work in higher eukaryotes also demonstrated a profound defect in this checkpoint in the absence of Bub3 (20)(21)(22)(23). Recently, Bub3 has been shown to bind the phospho MELT motif on KNL-1 for kinetochore localization of Bub1 (budding uninhibited by benzimidazole 1), disruption of which caused a defective checkpoint (24)(25)(26)(27) with Bub1 binding to kinetochores apparently required for binding of other checkpoint proteins (28)(29)(30)(31)(32).…”

mentioning

confidence: 99%

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Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling

Han¹,

Vitre²,

Fachinetti³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance The mitotic checkpoint (or the spindle assembly checkpoint) ensures genome integrity by preventing premature chromosome segregation. The pathway is triggered locally by kinetochores, multiprotein complexes assembled onto centromeres. Unattached kinetochores produce Mad2 bound to Cdc20, the mitotic activator of the E3 ubiquitin ligase APC/C. The initial Mad2–Cdc20 complex is then converted into the final mitotic checkpoint inhibitor Bub3–BubR1–Cdc20 that blocks APC/C (anaphase promoting complex or cyclosome)-dependent ubiquitination of cyclin B and securin, thereby stabilizing them and preventing an advance to anaphase. In this study, we identify dual mechanisms by which Bub3 promotes mitotic checkpoint signaling. Bub3 binding to BubR1 promotes two distinct BubR1–Cdc20 interactions, one acting at unattached kinetochores and the other cytoplasmically to facilitate production of the mitotic checkpoint inhibitor.

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Section: Resultssupporting

confidence: 81%

mentioning

confidence: 99%

Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling

Han¹,

Vitre²,

Fachinetti³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Logarinho et al (54) confirmed and extended these results. They showed that 10 -30% of cells depleted of Bub1, Bub3, and BubR1 at metaphase had more than four misaligned chromosomes.…”

Section: Promoting Proper Kinetochore-microtubule Attachment By Spindmentioning

confidence: 72%

Kinase Signaling in the Spindle Checkpoint

Kang

2009

Journal of Biological Chemistry

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The spindle checkpoint is a cell cycle surveillance system that ensures the fidelity of chromosome segregation. In mitosis, it elicits the "wait anaphase" signal to inhibit the anaphase-promoting complex or cyclosome until all chromosomes achieve bipolar microtubule attachment and align at the metaphase plate. Because a single kinetochore unattached to microtubules activates the checkpoint, the wait anaphase signal is thought to be generated by this kinetochore and is then amplified and distributed throughout the cell to inhibit the anaphase-promoting complex/cyclosome. Several spindle checkpoint kinases participate in the generation and amplification of this signal. Recent studies have begun to reveal the activation mechanisms of these checkpoint kinases. Increasing evidence also indicates that the checkpoint kinases not only help to generate the wait anaphase signal but also actively correct kinetochore-microtubule attachment defects.

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“…49,71,72 The next proteins that bind to kinetochors are Bub3 and BubR1 (mammalian homolog of yeast Mad3). 73 As a platform, Bub3 associates with Mad2, Mad3 and Cdc20, which directly inhibit anaphase promoting complex/cyclosome (APC/C). 74,75 BubR1 is required for kinetochore loading of Mad1 and Mad2.…”

Section: Reviewmentioning

confidence: 99%

Molecular insights into mechanisms regulating faithful chromosome separation in female meiosis

Yin

Sun²,

Schatten³

et al. 2008

Cell Cycle

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The faithful segregation of chromosomes into daughter cells in meiosis is crucial to produce healthy progeny. In gametogenesis, two consecutive rounds of chromosome separation occur with only one round of DNA replication, and the chromosome number is reduced to half to produce haploid gametes. Here, we discuss the molecular mechanisms underlying faithful chromosome separation in meiosis from three aspects: spindle checkpoint, two-step releases of cohesion, and the specific space-time protection of cohesin.

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The Human Spindle Assembly Checkpoint Protein Bub3 Is Required for the Establishment of Efficient Kinetochore–Microtubule Attachments

Cited by 93 publications

References 65 publications

Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling

Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling

Kinase Signaling in the Spindle Checkpoint

Molecular insights into mechanisms regulating faithful chromosome separation in female meiosis

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