The humic acid-induced changes in the root concentration of nitric oxide, IAA and ethylene do not explain the changes in root architecture caused by humic acid in cucumber

Mora, Verónica; Baigorri, Roberto; Bacaicoa, Eva; Zamarreño, Ángel M.; García-Mina, José M.

doi:10.1016/j.envexpbot.2011.10.001

Cited by 93 publications

(107 citation statements)

References 33 publications

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“…The concentration of the main phytoregulators in SHA composition was assessed using HPLC-mass spectrometry (MS) as described in Aguirre et al (2009). Finally, SHA was characterized using 13 C NMR dynamic light scattering and elemental analysis as described in Mora et al (2012) (Supplemental Figs. S1 and S2; Supplemental Table S3).…”

Section: Methodsmentioning

confidence: 99%

“…or water reservoirs (rivers, lakes, etc. ), extracted with alkaline water solutions, or isolated by resin fixation, reverse osmosis, or ultrafiltration (Alberts and Takács, 2004) Zandonadi et al, 2010;Canellas et al, 2011;Trevisan et al, 2011;Mora et al, 2012Mora et al, , 2014a, ethylene, and abscisic acid (ABA) in roots (Mora et al, 2012(Mora et al, , 2014a as well as cytokinins in shoots (Mora et al, 2010(Mora et al, , 2014b. Recently, Mora et al, 2014a showed that the HA ability to enhance both shoot growth and ABA root concentration in cucumber was regulated by IAA and NO root signaling pathways.…”

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confidence: 99%

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ABA-regulation of root hydraulic conductivity and aquaporin gene- expression is crucial to the plant shoot rise caused by rhizosphere humic acids

et al. 2015

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The physiological and metabolic mechanisms behind the humic acid-mediated plant growth enhancement are discussed in detail. Experiments using cucumber (Cucumis sativus) plants show that the shoot growth enhancement caused by a structurally well-characterized humic acid with sedimentary origin is functionally associated with significant increases in abscisic acid (ABA) root concentration and root hydraulic conductivity. Complementary experiments involving a blocking agent of cell wall pores and water root transport (polyethylenglycol) show that increases in root hydraulic conductivity are essential in the shoot growth-promoting action of the model humic acid. Further experiments involving an inhibitor of ABA biosynthesis in root and shoot (fluridone) show that the humic acid-mediated enhancement of both root hydraulic conductivity and shoot growth depended on ABA signaling pathways. These experiments also show that a significant increase in the gene expression of the main root plasma membrane aquaporins is associated with the increase of root hydraulic conductivity caused by the model humic acid. Finally, experimental data suggest that all of these actions of model humic acid on root functionality, which are linked to its beneficial action on plant shoot growth, are likely related to the conformational structure of humic acid in solution and its interaction with the cell wall at the root surface.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

ABA-regulation of root hydraulic conductivity and aquaporin gene- expression is crucial to the plant shoot rise caused by rhizosphere humic acids

et al. 2015

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“…In this regard, HS of both relative high and low molecular mass isolated from different sources in several exposure times and concentrations were able to exert its hormone-like activity in maize, tomato, Arabidopsis and cucumber (Dobbss et al 2007, Zandonadi et al 2007, Carletti et al 2008, Elena et al 2009, Mora et al 2010, Schiavon et al 2010, Zandonadi et al 2010, Trevisan et al 2011, Mora et al 2012. The problem of comparing these and others results in the literature is the heterogeneity of HS extraction methods and chemical characteristics, and also the plant treatment approaches.…”

Section: Evidences For the Hormone-like Activity Of Humusmentioning

confidence: 95%

Plant physiology as affected by humified organic matter

Zandonadi¹,

Santos²,

Busato³

et al. 2013

Theor. Exp. Plant Physiol.

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ABSTRACT:Since the beginning of Human civilization, the soil organic matter has been used as plant growth promoter and/or regulator.Indeed, early in plant science history, even before the auxin concept has been established, the term "auximones" was coined to describe plant growth promoting humic acids derived from peat. Despite of this, until the end of the 20 th century, humic substances remained as some of the most neglected environment signals in plant physiology research. However, this scenario has changed in last decade with the discovery that the major systems of energy transduction of the plant cell membranes, the proton pumps, can be tightly orchestrated by humic substances just as elicited by a hormonal signaling. Differential activations of both plasma membrane (PM H + -ATPase) and vacuolar pumps (V-ATPase and H + -PPase) are modulated by humic substances triggering ion signatures related to specific patterns of plant growth and development. Phytohormones have been found to be associated with this humus bioactivity, and nitric oxide acting as a second messenger in a signaling pathway in which plants can sense the soil environment to cope with specific conditions. In this review, we discuss some of the most influential data available in literature, which have shaped this underexplored interface between the chemistry of the organic matter and the plant physiology. The key role of organic matter in the sustainable agriculture will also be highlighted from a biochemical perspective of the plant cell responses to biofertilization, specially in tropical environments.

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“…NO was also shown to be involved in Arabidopsis root hair formation in both initiation and elongation processes [31,32]. The application of HS on roots of cucumber plants caused a primary increase in NO accumulation and it was associated by Mora et al [33] with the expression of the following morphological root changes: (1) increase in the number of secondary roots that were measured in the medium region of root system, (2) increase in root thickness, and (3) increase in root fresh weight.…”

Section: Reviewmentioning

confidence: 96%

Physiological responses to humic substances as plant growth promoter

Canellas

Olivares

2014

Chem Biol Techn Agric

387

243

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Humic substances (HS) have been widely recognized as a plant growth promoter mainly by changes on root architecture and growth dynamics, which result in increased root size, branching and/or greater density of root hair with larger surface area. Stimulation of the H + -ATPase activity in cell membrane suggests that modifications brought about by HS are not only restricted to root structure, but are also extended to the major biochemical pathways since the driving force for most nutrient uptake is the electrochemical gradient across the plasma membrane. Changes on root exudation profile, as well as primary and secondary metabolism were also observed, though strongly dependent on environment conditions, type of plant and its ontogeny. Proteomics and genomic approaches with diverse plant species subjected to HS treatment had often shown controversial patterns of protein and gene expression. This is a clear indication that HS effects of plants are complex and involve non-linear, cross-interrelated and dynamic processes that need be treated with an interdisciplinary view. Being the humic associations recalcitrant to microbiological attack, their use as vehicle to introduce beneficial selected microorganisms to crops has been proposed. This represents a perspective for a sort of new biofertilizer designed for a sustainable agriculture, whereby plants treated with HS become more susceptible to interact with bioinoculants, while HS may concomitantly modify the structure/activity of the microbial community in the rhizosphere compartment. An enhanced knowledge of the effects on plants physiology and biochemistry and interaction with rhizosphere and endophytic microbes should lead to achieve increased crop productivity through a better use of HS inputs in Agriculture.

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The humic acid-induced changes in the root concentration of nitric oxide, IAA and ethylene do not explain the changes in root architecture caused by humic acid in cucumber

Cited by 93 publications

References 33 publications

ABA-regulation of root hydraulic conductivity and aquaporin gene- expression is crucial to the plant shoot rise caused by rhizosphere humic acids

ABA-regulation of root hydraulic conductivity and aquaporin gene- expression is crucial to the plant shoot rise caused by rhizosphere humic acids

Plant physiology as affected by humified organic matter

Physiological responses to humic substances as plant growth promoter

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