The Hybridization Barrier between Herbaceous Medicago sativa and Woody M. arborea Is Weakened by Selection of  Seed Parents

Bingham, E. T.; Armour, D. J.; Irwin, J. A. G.

doi:10.3390/plants2020343

Cited by 13 publications

(41 citation statements)

References 15 publications

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“…The seeds of M. arborea, M. sativa and Alborea used in the present study was obtained from E. Bingham from the Agronomy Department, University of -Madison-Wisconsin"Wisconsin, United States. The development of Alborea was described in detail by [22,24].…”

Section: Plant Materialsmentioning

confidence: 99%

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Growth, Physiological, Biochemical, and Transcriptional Responses to Drought Stress in Seedlings of Medicago sativa L., Medicago arborea L. and Their Hybrid (Alborea)

et al. 2019

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Medicago sativa L. is a tetraploid perennial forage legume of great agronomical interest. The increasing need for its use under water-deficit conditions as well as low-input systems demands further improvement of its drought tolerance. On the other hand, Medicagoarborea L. is a perennial leguminous shrub, which is knownas a drought-tolerant species. In the present study, drought stress responses of the aforementioned medicago species, along with their hybrid, named Alborea, were comparatively assayed at the morphological, physiological, biochemical, and transcriptional levels. In particular, transcript abundance of representative genes that: (a) control ion transport, intracellular Na + /H + antiporters(NHX1) and rare cold inducible2A (RCI2A); (b) have an osmotic function ∆1-pyrroline-5-carboxylate synthetase 1 (P5CS1); and (c) participate in signaling pathways and control cell growth and leaf function stress-induced mitogen-activated protein kinases kinases (SIMKK), Zinc Finger (ZFN), apetala2/ethylene-responsive element binding (AP2/EREB), basic leucine zipper (bzip) and Medicago sativa Helicase 1(MH1) were evaluated. Under well-watered conditions, the studied population of Alborea showed the highest stem elongation rate and photosynthetic rate that were dramatically reduced under drought conditions compared to M. sativa and M. arborea. Under drought conditions, the studied population of M. arborea showed less reduction of relative water content, all gas-exchange parameters, less lipid peroxidation, and more antioxidant capacity. Moreover, transcriptional analysis demonstrated that the population of M. arborea exhibited significantly higher transcript levels of drought-responsive genes in both leaves and roots under drought stress conditions. M. sativa has better antioxidant capacity than Alborea and had a higher induction of stress-related genes, thus it performs better than Alborea under drought conditions. Among the studied genes, it seems that AP2/EREB play a critical role in the response of the studied population to drought stress.

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Section: Plant Materialsmentioning

confidence: 99%

“…Researchers have attempted the introgression of favorable traits from M. arborea genome to M. sativa such as drought tolerance. Hybrids between M. sativa and M. arborea named Alborea have been produced in the USA and Australia [22,23].…”

Section: Introductionmentioning

confidence: 99%

Growth, Physiological, Biochemical, and Transcriptional Responses to Drought Stress in Seedlings of Medicago sativa L., Medicago arborea L. and Their Hybrid (Alborea)

et al. 2019

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“…The M. sativa parent population used in this study was a hybrid of the two M. sativa parents. Two M. sativa parents were used in crosses of M. sativa × M. arborea to produce 27 initial Alborea hybrids [27]. The Alborea population used in this study was developed from intercrosses of 20 initial hybrids using methods described by Reference [28].…”

Section: Plant Materialsmentioning

confidence: 99%

“…However, there is limited research on the molecular mechanisms related to its salt tolerance. Hybrids between M. sativa and M. arborea were produced in the United States of America (USA) and Australia, and are named Alborea, a high-yielding hybrid [27,28]. There is evidence that the high-yielding hybrids in some cases are more sensitive to stresses compared to their parents [29].…”

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confidence: 99%

Seedling Growth and Transcriptional Responses to Salt Shock and Stress in Medicago sativa L., Medicago arborea L., and Their Hybrid (Alborea)

et al. 2018

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Salinity is a major limiting factor in crop productivity worldwide. Medicago sativa L. is an important fodder crop, broadly cultivated in different environments, and it is moderately tolerant of salinity. Medicago arborea L. is considered a stress-tolerant species and could be an important genetic resource for the improvement of M. sativa’s salt tolerance. The aim of the study was to evaluate the seedling response of M. sativa, M. arborea, and their hybrid (Alborea) to salt shock and salt stress treatments. Salt treatments were applied as follows: salt stress treatment at low dose (50 mM NaCl), gradual acclimatization at 50–100 and 50–100–150 mM NaCl, and two salt shock treatments at 100 and 150 mM NaCl. Growth rates were evaluated in addition to transcriptional profiles of representative genes that control salt uptake and transport (NHX1 and RCI2A), have an osmotic function (P5CS1), and participate in signaling pathways and control cell growth and leaf function (SIMKK, ZFN, and AP2/EREB). Results showed that the studied population of M. sativa and M. arborea performed equally well under salt stress, whereas that of M. sativa performed better under salt shock. The productivity of the studied population of Alborea exceeded that of its parents under normal conditions. Nevertheless, Alborea was extremely sensitive to all initial salt treatments except the low dose (50 mM NaCl). In addition, significantly higher expression levels of all the studied genes were observed in the population of M. arborea under both salt shock and salt stress. On the other hand, in the population of M. sativa, NHX1, P5CS1, and AP2/EREB were highly upregulated under salt shock but to a lesser extent under salt stress. Thus, the populations of M. sativa and M. arborea appear to regulate different components of salt tolerance mechanisms. Knowledge of the different parental mechanisms of salt tolerance could be important when incorporating both mechanisms in Alborea populations.

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“…Up to now, the intraspecific hybridization with the wild-growing subspecies of legumes has been used most successfully in alfalfa (Bingham et al 2013;Milić et al 2016). Intersubspecific sativa by falcata alfalfa (Medicago sativa L.) hybrids offer a means of improving alfalfa dry-matter yields (Riday & Brummer 2004).…”

Section: Introductionmentioning

confidence: 99%

The potential breeding value of snow clover (Trifolium pratense ssp. nivale (W.D.J. Koch) Arcang.) and red clover (Trifolium pratense ssp. sativum) hybrids

Naydenova¹,

Vasileva²

2019

TJB

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The potential breeding value of snow clover (Trifolium pratense ssp. nivale (W.D.J. Koch) Arcang.) and red clover (Trifolium pratense ssp. sativum) hybrids Naydenova, G. & Vasileva, V. (2019): The potential breeding value of snow clover (Trifolium pratense ssp. nivale (W.D.J. Koch) Arcang.) and red clover (Trifolium pratense ssp. sativum) hybrids. -Thaiszia -J. Bot. 29 (2): 217-224Abstract: Eight red clover subspecies are registered in Europe but it can be considered that the high genetic diversity and local genetic adaptation existing at a subspecies level is unused. The experiment aimed at studying agronomical traits of the hybrids obtained from crosses of wild subspecies of Trifolium pratense -snow clover (Trifolium pratense ssp. nivale (W.D.J. Koch) Arcang.) and cultivated red clover (Trifolium pratense ssp. sativum). Under field conditions, two snow clover half-sib families -F 1 generation of crossbreeding were selected and compared to the paternal variety Nika 11 for three years. According to results, the size, habit of growth as well as dry matter productivity at spring growth of the F 1 hybrids were similar to the cultivated red clover. In summer regrowth, they were less productive. In terms of a type and rate of development the hybrids were similar to snow clover. From morphological point of view, the families proved of interest with its abundant leafiness, which renders them suitable for breeding in pasture direction. The hybrids are characterized by an icreased number of seeds per inflorescence and seed yield. The open-pollinated seeds of families were used for the formation of a population that represented the F 2 generation progenies. The phenotypic variation in the first vegetation of F 2 generation was high for plant flower colour, growth habit, and degree of pubescence. It can be considered that this population contains a high genetic variation and is suitable for development of complex hybrid breeding populations.

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The Hybridization Barrier between Herbaceous Medicago sativa and Woody M. arborea Is Weakened by Selection of Seed Parents

Cited by 13 publications

References 15 publications

Growth, Physiological, Biochemical, and Transcriptional Responses to Drought Stress in Seedlings of Medicago sativa L., Medicago arborea L. and Their Hybrid (Alborea)

Growth, Physiological, Biochemical, and Transcriptional Responses to Drought Stress in Seedlings of Medicago sativa L., Medicago arborea L. and Their Hybrid (Alborea)

Seedling Growth and Transcriptional Responses to Salt Shock and Stress in Medicago sativa L., Medicago arborea L., and Their Hybrid (Alborea)

The potential breeding value of snow clover (Trifolium pratense ssp. nivale (W.D.J. Koch) Arcang.) and red clover (Trifolium pratense ssp. sativum) hybrids

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