The Hydra assay as an early screen for teratogenic potential

Wilby, Owen K.; Tesh, J.M.

doi:10.1016/0887-2333(90)90119-e

Cited by 42 publications

(30 citation statements)

References 2 publications

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“…Good correlation was found between the in vitro Hydra regeneration assay and teratogenicity in vivo, as reported by Bowden et al, (1995) and Wilby and Tesh (1990), who proposed it as a screening tool for teratogenicity. Furthermore the combination of Hydra developmental hazard index (A/D ratio) and rat whole embryo culture test have been recommended for use together to facilitate the rapid detection and ranking of hazardous chemicals associated with complex mixtures of chemical waste (Mayura et al, 1991;Yang et al, 1993).…”

Section: Regenerationsupporting

confidence: 68%

Hydra, a model system for environmental studies

Quinn¹,

Gagné²,

Blaise³

2012

Int. J. Dev. Biol.

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Hydra have been extensively used for studying the teratogenic and toxic potential of numerous toxins throughout the years and are more recently growing in popularity to assess the impacts of environmental pollutants. Hydra are an appropriate bioindicator species for use in environmental assessment owing to their easily measurable physical (morphology), biochemical (xenobiotic biotransformation; oxidative stress), behavioural (feeding) and reproductive (sexual and asexual) endpoints. Hydra also possess an unparalleled ability to regenerate, allowing the assessment of teratogenic compounds and the impact of contaminants on stem cells. Importantly, Hydra are ubiquitous throughout freshwater environments and relatively easy to culture making them appropriate for use in small scale bioassay systems. Hydra have been used to assess the environmental impacts of numerous environmental pollutants including metals, organic toxicants (including pharmaceuticals and endocrine disrupting compounds), nanomaterials and industrial and municipal effluents. They have been found to be among the most sensitive animals tested for metals and certain effluents, comparing favourably with more standardised toxicity tests. Despite their lack of use in formalised monitoring programmes, Hydra have been extensively used and are regarded as a model organism in aquatic toxicology.

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Section: Regenerationsupporting

confidence: 68%

Hydra, a model system for environmental studies

Quinn¹,

Gagné²,

Blaise³

2012

Int. J. Dev. Biol.

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“…Gelatinous zooplankton, which include cnidarians, ctenophores, and pelagic tunicates (salps, doliolids, and pyrosomes), are not represented in routine ecotoxicology, and data regarding their use as bioassay model organism are very scarce in literature. Cnidarians (e.g., hydras, colonial hydroids, sea anemones, scleractinian corals) are the major benthic organisms used in toxicity testing for investigating budding, regeneration, gametogenesis, mucus production, and larval metamorphosis (Wilby & Tesh, 1990;Kusui & Blaise, 1999;Pollino & Holdway, 1999;Karntanut & Pascoe, 2000;Holdaway et al, 2001;Pascoe et al, 2003;Downs et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of neurotoxic compounds on ephyrae of Aurelia aurita jellyfish

et al. 2015

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The aim of this study was to analyze the toxicity of two neurotoxic compounds on the ephyra stage of the Scyphozoan jellyfish Aurelia aurita, an innovative and sensitive model organism recently proposed in ecotoxicological bioassays. Indeed, jellyfish play an important role in the marine ecosystem, being a key component of the gelatinous zooplankton and of the marine food web, but are not represented in routine ecotoxicology. In this study, ephyrae were exposed to several concentrations (0, 0.1, 0.5, 1, 5, 10, 50 mg l -1 ) of eserine (ES) and chlorpyrifos (CPF), a carbamate and an organophosphorous compound, respectively, in order to analyze their toxic effect on this model organism. Acute (% of immobility) and behavioral (% alteration of frequency of pulsations) end-points were investigated after 24 and 48 h of exposure. Exposure to both compounds caused a dosedependent effect, and for each end-point, it was possible to quantify these effects by means of EC 50 . Results highlighted how these compounds reported a high toxicity for marine organisms and exerted their toxic effect on this innovative biological model (in particular, CPF resulted to be more toxic than ES), which proved to be more sensitive than other marine invertebrates commonly used for ecotoxicological bioassays.

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“…Teratogenicity assays have been carried out in Hydra by following phenotype changes [17][18][19][20] . These assay results have shown clearly that some chemicals induce deformities in Hydra polyps.…”

Section: Axis Formation and Teratogenicity Assaysmentioning

confidence: 99%

Hydra, a candidate for an alternative model in environmental genomics

Yum

Woo

Lee

et al. 2014

Mol. Cell. Toxicol.

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The need for nonmammalian and nonvertebrate experimental systems for toxicology is increasing because of growing concern about animal welfare. Hydra, a genus of freshwater cnidarian, has been used in classical toxicology for more than 40 years and has been recommended as a standard model for ecotoxicology. Functional assays using Hydra homologues of the signal molecules and transcription factors of other metazoans have demonstrated that Hydra shares the common molecular mechanisms for pattern formation with bilaterian animals. The availability of the Hydra genome and mRNA information has meant that it can be used as a model organism in biological sciences. The purpose of this review is to explore recent developments in our understanding of the molecular aspects of axis formation, bud formation, regeneration, and the nervous system in Hydra and to introduce Hydra as an alternative animal model in environmental toxicogenomics for predicting the toxicity of environmental pollutants at the molecular level.

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The Hydra assay as an early screen for teratogenic potential

Cited by 42 publications

References 2 publications

Hydra, a model system for environmental studies

Hydra, a model system for environmental studies

Effect of neurotoxic compounds on ephyrae of Aurelia aurita jellyfish

Hydra, a candidate for an alternative model in environmental genomics

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