The Hydroalcoholic Extract of Leaves of Mandevilla moricandiana Induces NO-Mediated Vascular Relaxation

Abstract

Natural products extracted from plants represent a valuable source of new bioactive substances. Many studies describe the potential of plant products for the treatment of cardiovascular diseases. Species of the genus have been studied for their biological activities, mainly as antioxidant, anti-inflammatory, and vasorelaxant. However, the phytochemical and pharmacological profiles of have not been investigated yet. The aim of this study was to evaluate the vasodilator effect of the hydroalcoholic extract of the leaves of , as well as its chemical profile. Chemical analysis and quantification of major compounds were performed by HPLC analysis. Total flavonoid content was quantified based on rutin equivalents, and major compounds were identified based on HPLC-DAD-MS analysis. leaf extract-induced vasodilation was investigated in rat aortic rings precontracted with phenylephrine. The total flavonoids were quantified as 3.25 ± 0.11 % w/w of the hydroalcoholic leaf extract, and HPLC-DAD-MS allowed for the identification of luteolin and quercetin glycosides. The maximal relaxant effect of the hydroalcoholic leaf extract was 86.07 ± 1.68 % at a concentration of 30 µg/mL (p< 0.05; n = 6). The concentration of hydroalcoholic extract of the leaves of necessary to reduce phenylephrine-induced contractions of the endothelium-intact aorta by 50 % was 0.82 ± 0.10 µg/mL. leaf extract-induced vasodilation was abolished in aortas pretreated with NG-nitro-L-arginine methyl ester and 1H-[1,2,4]oxadiazolo-[4,3-]quinoxalin-1-one. In addition, diphenhydramine partially inhibited the effect of the hydroalcoholic extract of the leaves of . Thus,-induced relaxation depends on the endothelium and on the activation of the nitric oxide/cyclic GMP pathway, with the involvement of endothelial histamine H receptors. Luteolin and quercetin glycosides seem to contribute to the extract activity.

Phytochemical Study of Tapirira guianensis Leaves Guided by Vasodilatory and Antioxidant Activities

“…The LC system, as previously described [ 45 ], was coupled to a mass spectrometer ESI-IT (Bruker Daltonics, Billerica, MA, USA), fitted with an electrospray ionization source operating in the positive mode, and an ion trap analyzer. The chromatographic conditions used were as follows: Luna C18 column (250 × 4.6 mm, 5 μm, Phenomenex, Torrance, CA, USA), sample injection volume of 10 μL at 1 mg/mL, flow rate 1.0 mL/min at 25 °C, and H 2 O containing 0.1% ( v / v ) formic acid (solvent A) and acetonitrile (solvent B) as the mobile phase.…”

Astragaloside IV Prevents Cardiac Remodeling in the Apolipoprotein E-Deficient Mice by Regulating Cardiac Homeostasis and Oxidative Stress

“…We postulated that certain common signal transduction pathways and regulators were the targets of AST-IV for its beneficial effects on hypercholesterolemia. Previous studies have confirmed that AST-IV could reverse or delay cardiac remodeling caused by various reasons, such as viral infection [35], isoproterenol (ISO) [26, 31, 53] and lipopolysaccharide (LPS) [27]. AST-IV protected against ISO-induced cardiac hypertrophy via inhibiting p65 translocation and increasing PPARγ coactivator 1α (PGC-1α) [28].…”

“…AST-IV prevented myocardial fibrosis in coxsackievirus B3-induced dilated cardiomyopathy via down-regulating TGF-β1, Smad4 and p-Smad2/3 expressions [35]. AST-IV attenuated LPS-induced cardiac hypertrophy through inhibiting the activation of Ca 2+ /calcineurin, GATA-4, and the nuclear translocation of NFAT-3 [27]. AST-IV strongly blocked Rheb/mTORC1 signaling pathway and reduced its downstream phospho-S6K levels in myocardial infarction (MI) and transverse aortic constriction (TAC) mice model [30].…”

Ethyl Acetate Fraction and Isolated Phenolics Derivatives from Mandevilla moricandiana Identified by UHPLC-DAD-ESI-MSn with Pharmacological Potential for the Improvement of Obesity-Induced Endothelial Dysfunction

“…The MM extract was solubilized in 350 mL of MeOH/distilled water (9:1) under agitation and was submitted to successive liquid–liquid partitions with organic solvents in the following eluotropic order: n-hexane (MMHF), dichloromethane (MMDCMF), ethyl acetate (MMEAF), and n-butanol (MMBF). After partition with n-butanol, the remaining aqueous fraction (MMAF) was obtained, and it was submitted to freezing followed by lyophilization (Freeze Dryer: L101 Liotop, São Paulo, Brazil), as previously described by our group [ 31 ].…”

“…Among our efforts to study the plant biodiversity of this park, we have previously shown that the hydroalcoholic extract of leaves of M. moricandiana induces NO-dependent vasodilation, partially through the activation of histamine (H1) and estrogen (ERα) receptors. This effect seemed to be mediated by flavonoids that are present in the extract [ 31 ]. Another aspect that encourages further chemical and pharmacological studies about M. moricandiana is that micropropagation and in vitro conservation techniques have been previously described [ 32 , 33 ] and could support the sustainable management of the species.…”