“…The precise nature of the hydrodynamic forces acting on the particles is potentially very complex: in the past 50þ years, since the pioneering work of Segre and Silberberg, [26][27][28] descriptions of forces on particles in many types of flows have been developed, from simple shear flows 29 to more general background flows, 30 sometimes explicitly taking into account the presence of a nearby wall, 31,32 or the explicit time dependence of the flow. 33,34 Even the behavior of common microparticles in the transport flow through ordinary, ubiquitous microfluidic channels still reveals novel insights today. 35,36 Because the bubbles are driven acoustically in our experiments, one may suspect acoustic radiation forces at work; however, using our typical parameters to evaluate these forces 37 and translating them to particle displacements during passage near the bubble, we find that even our largest (a p ¼ 5 lm) particles would not be displaced perpendicular to streamlines by more than $100 nm if the particle and fluid densities were not matched, and far less under the density-matched conditions of our experiment.…”