The hydrolysis of 4-nitrophenol phosphate. A freshman class investigation

Abstract

Recently, several efforts to make the college chemistry laboratory a challenging problemoriented exercise have been reported (1-3). A somewhat similar attempt at Georgia State University has produced an integrated problem-based nine quarter sequence covering the first three years. Since a large number of the students passing through the first four quarters have interests associated with biological systems, a problem related to the biochemistry area was sought for one of the early quarters.Much of the free energy needed to drive biological processes is obtained by the hydrolysis of energy-rich phosphate ester derivatives found inside living cells. I n order to gain a better understanding of this hydrolysis process numerous studies (4-6) on simple phosphate (7) W a m~, A,, H m n~~n . P., AND SMITH. E. L.. "Principles of Biochemis try." MoGraw-Hill (4th sd.), 1968. Chap. 11. (8) Lownr, 0. H., AND LOPEZ.

Two spectrophotometric experiments with alpha-chymotrypsin

“…Enough experiments could be devised so that it would be possible to have each member of a class work on a different project, with one common goal. Such class oriented activities have provoked much comment (26)(27)(28).…”

Preparation of immobilized enzymes, and determination of their pH activity profile: An integrated organic-biology experiment

“…Determination of their pH Activity Profile An integrated organic-biology experiment One of the problems facing those in charge of undergraduate organic laboratories is finding or devising experiments appropriate for classes that have a high percentage of biological science majors. Recently a number of experiments have appeared with these goals in mind (1)(2)(3)(4)(5).…”

“…( 3)) and subsequently converted to a diazonium salt by reaction with NaNC>2/HCl (eqn. (4)). The glass has now been modified such that it is ready to be coupled with the enzyme.…”