The Hydrophobicity of AIE Dye Facilitates DNA Condensation for Carrier‐Free Gene Therapy

Chen, Zhe; Wei, Zixiang; Fan, Xiaoxing; Zhang, Chao; Lu, Jingxiong; Wang, Zhiming; Tian, Leilei

doi:10.1002/adfm.202207845

Cited by 10 publications

(4 citation statements)

References 44 publications

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“…[11] Polyanionic DNA nanomaterials are known to target SRs, followed by internalization along the endosome-lysosome pathway via caveolae-mediated endocytosis. [12] Since SRs are abundant on cancer cells, various nucleic acid nanodevices have been established to co-opt SRs for intracellular delivery of therapeutic agents [13] and lysosome-targeted delivery of chemical reporters. [14] However, the potential of SRs as an applicable LTR to mediate protein degradation has not been explored yet.…”

mentioning

confidence: 99%

Dendronized DNA Chimeras Harness Scavenger Receptors To Degrade Cell Membrane Proteins

Zhu

Wang

et al. 2023

Angew Chem Int Ed

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Bispecific chimeras bridging cell membrane proteins with lysosome‐trafficking receptors (LTRs) provide an effective therapeutic approach through lysosomal degradation of disease‐relevant targets. Here, we report a novel dendronized DNA chimera (DENTAC) strategy that uses a dendritic DNA to engage cell surface scavenger receptors (SRs) as LTR. Using bioorthogonal strain‐promoted alkyne‐azide cycloaddition to conjugate the dendritic DNA with protein binder, the resulting DENTAC is able to traffic the protein target into the lysosome for elimination. We demonstrated the utility of DENTAC by degrading oncogenic membrane nucleolin (NCL) and epidermal growth factor receptor (EGFR). The anti‐cancer application of NCL‐targeting DENTAC was validated in a mouse xenograft model of lung cancer. This work thus presents a new avenue for rapid development of potent degraders against membrane proteins, with also broad research and therapeutic prospects.

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confidence: 99%

Dendronized DNA Chimeras Harness Scavenger Receptors To Degrade Cell Membrane Proteins

Zhu

Wang

et al. 2023

Angew Chem Int Ed

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“…This copolymer exhibited distinct self-assembly behaviors for generating polyplex with DNA, along with the AIE emissions with tunable colors for monitoring. 159 In addition, the emission related to charged states of dyes can be modulated by the formation of a polyplex between the negatively charged DNA and the positively charged sulfonium segments in the shell. These works have demonstrated the potential of AIE-based DNA nanostructures for biological applications.…”

Section: Tion Of Photostable Biosensing Nanodevices For Biological Ap...mentioning

confidence: 99%

Advances of fluorescent DNA nanostructures in biomedical applications

Shen,

Cao,

Xing

et al. 2024

TIMS

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With the rapid development of DNA nanotechnology, the emergence of fluorescent DNA nanostructures (FDNs) has enlarged the biological applications. FDNs have great advantages of precise localization and real-time tracing in bioimaging. In this review, the recent biomedical developments of FDNs have been reviewed, including the design of FDNs, and the corresponding applications on biomarker sensing, bioimaging, cancer diagnosis and therapy. Firstly, the development of DNA nanostructures and the corresponding DNA-based nanomaterials were briefly introduced. Simultaneously, to make a better demonstration, the background and theory of the fluorescence detections were briefly introduced. Thereafter, the synthetic strategies of DNA nanostructure were summarized and classified, which facilitated the multiple functionalizations for sensing and bioimaging. Subsequently, the biomedical applications of FDNs are comprehensively summarized based on different detection strategies, including fluorescence resonance energy transfer (FRET), aggregation-induced emission (AIE), near-infrared (NIR)-photoactivation, nucleic acid amplification (NAT), small fluorescent dyes loading, and surface plasmon resonance (SPR) technologies. Finally, an insight into the challenges and future perspectives is provided. As reviewed, FDNs are important tools in precision medicine, showing great potential in both in vivo and in vitro cancer diagnosis and treatments. Undoubtedly, FDN-based technology is a promising strategy for constructing versatile nanodevices in biological applications and will excel in human healthcare.

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“…[15] Modifying DNA ends with hydrophobic materials such as AIE materials, block copolymers or cholesterol molecules can be an alternative to promote DNA self-assembly. [16][17][18] But additional chemical modifications increase the complexity of synthesis.…”

Section: Introductionmentioning

confidence: 99%

Self‐Assembled DNA Nanospheres Driven by Carbon Dots for MicroRNAs Imaging in Tumor via Logic Circuit

Chen,

Xia,

Liang

et al. 2024

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DNA nanostructures with diverse biological functions have made significant advancements in biomedical applications. However, a universal strategy for the efficient production of DNA nanostructures is still lacking. In this work, a facile and mild method is presented for self‐assembling polyethylenimine‐modified carbon dots (PEI‐CDs) and DNA into nanospheres called CANs at room temperature. This makes CANs universally applicable to multiple biological applications involving various types of DNA. Due to the ultra‐small size and strong cationic charge of PEI‐CDs, CANs exhibit a dense structure with high loading capacity for encapsulated DNA while providing excellent stability by protecting DNA from enzymatic hydrolysis. Additionally, Mg2+ is incorporated into CANs to form Mg@CANs which enriches the performance of CANs and enables subsequent biological imaging applications by providing exogenous Mg2+. Especially, a DNAzyme logic gate system that contains AND and OR Mg@CANs is constructed and successfully delivered to tumor cells in vitro and in vivo. They can be specifically activated by endogenic human apurinic/apyrimidinic endonuclease 1 and recognize the expression levels of miRNA‐21 and miRNA‐155 at tumor sites by logic biocomputing. A versatile pattern for delivery of diverse DNA and flexible logic circuits for multiple miRNAs imaging are developed.

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The Hydrophobicity of AIE Dye Facilitates DNA Condensation for Carrier‐Free Gene Therapy

Cited by 10 publications

References 44 publications

Dendronized DNA Chimeras Harness Scavenger Receptors To Degrade Cell Membrane Proteins

Dendronized DNA Chimeras Harness Scavenger Receptors To Degrade Cell Membrane Proteins

Advances of fluorescent DNA nanostructures in biomedical applications

Self‐Assembled DNA Nanospheres Driven by Carbon Dots for MicroRNAs Imaging in Tumor via Logic Circuit

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