The hypersusceptible antibiotic screening strain Staphylococcus aureus SG511-Berlin harbors multiple mutations in regulatory genes

Dietrich, Alina; Steffens, Ursula A. E.; Saß, Peter; Bierbaum, Gabriele

doi:10.1016/j.ijmm.2021.151545

Cited by 4 publications

(3 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a deferred activity test on blood agar, the original gallidermin producer Staphylococcus gallinarum Tü3928 (Kellner et al., 1988 ) produced only minimal activity against the VISA strains S. aureus Mu50 and S. aureus 137/93G (Figure 3A–C ), although it inhibited the growth of S. aureus SG511, a strain that is hypersensitive to many cationic peptides (Dietrich et al., 2021 ). In order to distinguish between the effects of gallidermin and a putative second active compound, the producer of epidermin, S. epidermidis Tü3298, which is immune to gallidermin and epidermin, was used as indicator strain in a deferred activity test (Figure S6 ).…”

Section: Resultsmentioning

confidence: 99%

A Staphylococcus capitis strain with unusual bacteriocin production

Szekat,

Josten,

Rickmeyer

et al. 2023

Microbial Biotechnology

Self Cite

View full text Add to dashboard Cite

Staphylococcus capitis is a member of the human and mammal skin microbiomes and is considered less harmful than Staphylococcus aureus. S. capitis subsp. urealyticus BN2 was isolated from a cat and expressed strong antibacterial activity against a range of Gram‐positive species, most notably including S. aureus strains with resistance to methicillin (MRSA) and strains with intermediate resistance to vancomycin (VISA). These latter strains are normally relatively resistant to bacteriocins, due to cell wall and cell membrane modifications. Genomic sequencing showed that the strain harboured at least two complete gene clusters for biosynthesis of antagonistic substances. The complete biosynthetic gene cluster of the well‐known lantibiotic gallidermin was encoded on a large plasmid and the mature peptide was present in isopropanol cell extracts. In addition, a chromosomal island contained a novel non‐ribosomal peptide synthetase (NRPS) gene cluster. Accidental deletion of two NRPS modules and partial purification of the anti‐VISA activity showed that this novel bacteriocin represents a complex of differently decorated, non‐ribosomal peptides. Additionally, a number of phenol‐soluble modulins (PSMs) was detected by mass spectrometry of whole cells. Producing these compounds, the strain was able to outcompete several S. aureus strains, including MRSA and VISA, in tube cultures.

show abstract

Section: Resultsmentioning

confidence: 99%

A Staphylococcus capitis strain with unusual bacteriocin production

Szekat,

Josten,

Rickmeyer

et al. 2023

Microbial Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Bacteria use a variety of DNA-mobilizing enzymes to facilitate environmental niche adaptation via horizontal gene transfer, and a study found that rpnA-E genes of E. coli encode nucleases involved in DNA recombination, but overexpression of RpnA (YhgA) to RpnD (YjiP) increased RecA-independent recombination, reduced cell viability, and induced the expression of reporter of DNA damage, while RpnE (YfaD) is inactive in these processes (Kingston et al, 2017). The ebh gene is one of the mutations with possible influence on resistance phenotype identified in the genome of S. aureus SG511 (Dietrich et al, 2021), and mutations that disrupt the ebh reading frame are associated with increased oxacillin and teicoplanin susceptibility (Cheng et al, 2014). In particular, the rpnE gene is a hub gene in the direct genetic effect network of E. coli and the indirect genetic effect network of S. aureus, indicating that this gene plays a pivotal role in the process of microbial interspecies interaction, and is worth further exploring its function and significance.…”

Section: Discussionmentioning

confidence: 99%

Competition-cooperation mechanism between Escherichia coli and Staphylococcus aureus based on systems mapping

Li,

Yin,

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionInterspecies interactions are a crucial driving force of species evolution. The genes of each coexisting species play a pivotal role in shaping the structure and function within the community, but how to identify them at the genome-wide level has always been challenging.MethodsIn this study, we embed the Lotka-Volterra ordinary differential equations in the theory of community ecology into the systems mapping model, so that this model can not only describe how the quantitative trait loci (QTL) of a species directly affects its own phenotype, but also describe the QTL of the species how to indirectly affect the phenotype of its interacting species, and how QTL from different species affects community behavior through epistatic interactions.ResultsBy designing and implementing a co-culture experiment for 100 pairs of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), we mapped 244 significant QTL combinations in the interaction process of the two bacteria using this model, including 69 QTLs from E. coli and 59 QTLs from S. aureus, respectively. Through gene annotation, we obtained 57 genes in E. coli, among which the genes with higher frequency were ypdC, nrfC, yphH, acrE, dcuS, rpnE, and ptsA, while we obtained 43 genes in S. aureus, among which the genes with higher frequency were ebh, SAOUHSC_00172, capF, gdpP, orfX, bsaA, and phnE1.DiscussionBy dividing the overall growth into independent growth and interactive growth, we could estimate how QTLs modulate interspecific competition and cooperation. Based on the quantitative genetic model, we can obtain the direct genetic effect, indirect genetic effect, and genome-genome epistatic effect related to interspecific interaction genes, and then further mine the hub genes in the QTL networks, which will be particularly useful for inferring and predicting the genetic mechanisms of community dynamics and evolution. Systems mapping can provide a tool for studying the mechanism of competition and cooperation among bacteria in co-culture, and this framework can lay the foundation for a more comprehensive and systematic study of species interactions.

show abstract

“…Genomic DNA was isolated from nine CmR mutants and their respective revertants using the Master Pure Gram-positive DNA purification kit (Epicentre Biotechnologies) with one modification: lysostaphin solution (30 ng/mL) was added during the lysis step. Whole-genome sequencing of the wild-type strain S. aureus SG511 (PacBio) was previously described ( 88 ). Illumina technology was used for resequencing of the mutant strains, according to Kehl et al ( 89 ).…”

Section: Methodsmentioning

confidence: 99%