The hypocholesterolaemic effect of milk yoghurt and soy-yoghurt containing bifidobacteria in rats fed on a cholesterol-enriched diet

El-Gawad, Ibrahim A. Abd; El-Sayed, E. M.; Hafez, S. A.; El-Zeini, Hoda M.; Saleh, Farag A.

doi:10.1016/j.idairyj.2004.06.001

Cited by 121 publications

(72 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These contradictions might be caused by different strains and amounts of administered probiotic cultures, no standardized application forms (milk products, sausage and capsules) and varying lengths of treatment periods. Moreover, a cholesterol-lowering effect of probiotics was more obviously, when hypercholesterolaemic individuals were chosen as subjects or when a cholesterol-enriched diet was given (Kie ling et al, 2002;Xiao et al, 2003;Abd El-Gawad et al 2005). There is no precise indication of the mechanism concerning a decrease in cholesterol levels in human.…”

Section: Discussionmentioning

confidence: 99%

“…However, there is little evidence that probiotic bacteria are able to reduce serum cholesterol in vivo. Most studies investigated the cholesterol-lowering effect in hypercholesterolaemic patients or after a high-fat diet (Bertolami et al, 1999;Kawase et al, 2001;Doncheva et al, 2002;Abd El-Gawad et al, 2005). There are few reports documenting the influence on normocholesterolaemic subjects (De Roos et al, 1999;Jahreis et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lactobacillus acidophilus 74-2 and Bifidobacterium animalis subsp lactis DGCC 420 modulate unspecific cellular immune response in healthy adults

Friedrich²,

et al. 2007

View full text Add to dashboard Cite

Objective: It was determined whether a combination of Lactobacillus acidophilus (L. acidophilus) 74-2 and Bifidobacterium animalis subsp lactis DGCC 420 (B. lactis 420) affect the faecal microbiota as well as immunological parameters and blood lipids in healthy adults. Design: A placebo-controlled, double-blinded, randomized crossover trial was conducted. Subjects: Twenty-six healthy volunteers (mean age 25 years) were recruited by advertising in academical buildings. All of them completed the study. Methods: After 3-week run-in period, half of the volunteers consumed 300 g/day of yoghurt supplement containing probiotic strains L. acidophilus 74-2 and B. lactis 420, and the other half received the placebo product for a period of 5 weeks. The two groups were crossed during the following 5-week period. Blood and faecal samples were collected at the end of each period. The faecal content of probiotic bacteria, faecal short-chain fatty acids (SCFA), serum lipids and plasma immune system biomarkers were evaluated. Results: Faecal proportions of L. acidophilus and of B. lactis increased significantly from 0.02 to 0.19 and 0.4 to 1.4% (Po0.05), respectively. Percentages of granulocytes and monocytes showing phagocytic activity were significantly elevated from 92 to 95% during probiotic intervention, whereas their oxidative burst activity and specific immune parameters remained unaffected. Fecal SCFA and serum cholesterol levels were not influenced by the probiotics. However, serum concentrations of triacylglyceroles decreased significantly by 11.6% (Po0.05) in the probiotic supplementation period. Conclusions: L. acidophilus and B. lactis were recovered in faeces in significantly elevated numbers after supplementation. They are able to modulate unspecific cellular immune response indicated by the increased phagocytic activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lactobacillus acidophilus 74-2 and Bifidobacterium animalis subsp lactis DGCC 420 modulate unspecific cellular immune response in healthy adults

Friedrich²,

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Likewise strains of B. animalis isolated from feces have shown to possess bile salt hydrolase activity (Tanaka et al, 1999). This has not been tested specifically for BB-12 but in vivo the hypocholesterolemic effect of bifidobacteria has recently been demonstrated in a study where rats were fed a cholesterol-rich diet along with BB-12 or Bifidobacterium longum BB-46-supplemented milk yoghurt as well as soy yoghurt (Abd El-Gawad et al, 2005). Clinical studies in humans are, however, ambiguous regarding the effects of probiotics on the concentrations of the blood lipids, showing both effect and absence there of.…”

Section: Discussionmentioning

confidence: 99%

“…Concerning the effect on blood lipids in vitro BB-12 showed the ability to deconjugate bile salt and thereby remove cholesterol from a culture medium (Klaver and van der Meer, 1993). Recently, BB-12 has shown the ability to reduce total cholesterol as well as very low-density lipoprotein þ low-density lipoprotein (LDL) cholesterol in rats fed a cholesterol-enriched diet (Abd El-Gawad et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Dose–response study of probiotic bacteria Bifidobacterium animalis subsp lactis BB-12 and Lactobacillus paracasei subsp paracasei CRL-341 in healthy young adults

Larsen

Nielsen²,

Kæstel³

et al. 2006

Eur J Clin Nutr

109

View full text Add to dashboard Cite

Objective: This study was performed to investigate the dose-response effects of supplementation with Bifidobacterium animalis subsp lactis (BB-12) and Lactobacillus paracasei subsp paracasei (CRL-431) on blood lipids, recovery from feces and bowel habits. Changes of the fecal microflora was analyzed in the 10 10 CFU/day probiotic and placebo group. Design: The study was designed as a randomized, placebo-controlled, double-blinded, parallel dose-response study. Subjects: Healthy young adults (18-40 years) were recruited by advertising in local newspapers. Of the 75 persons enrolled, 71 (46 women, 25 men, mean age 25.6 years (range 18-40 years)) completed the study. Intervention: The volunteers were randomly assigned into five groups receiving either placebo or a mixture of the two probiotics in the concentration of 10 8 , 10 9 , 10 10 or 10 11 CFU/day in 2 weeks run-in period, 3 weeks intervention and 2 weeks wash-out. Diary reporting bowel habits and well being (abdominal bloating, flatulence and headache) was kept for all 7 weeks and blood lipids, fecal recovery of BB-12 and CRL-431, as well as fecal microflora was tested before, immediately and 2 weeks after intervention. Results: The fecal recovery of BB-12 increased significantly (Po0.001) with increasing dose. In the group receiving 10 11 CFU/ day BB-12 was recovered from 13 out of 15 volunteers. CRL-431 was not recovered in any of the fecal samples. Supplementation with probiotics did not change the fecal bacterial composition. A significant linear increase in fecal consistency (looser stool) with increasing probiotic dose (P ¼ 0.018) was observed. No overall dose-response effect was found on the blood lipids. High doses of probiotics were well tolerated. Conclusion: A dose-related recovery of BB-12 from feces was observed. Sponsorship: The study was sponsored by Chr. Hansen A/S, Hoersholm, Denmark. Keywords: dose-response study; probiotics; cholesterol; recovery from feces; constipation European Journal of Clinical Nutrition IntroductionIt is generally accepted that the composition of the intestinal microflora influences the health and well being of humans. With the many publications showing beneficial effects of probiotic bacteria, there has been an increasing interest in the mechanism behind. Most clinical studies have tested only one dose of probiotics, ranging from 4 Â 10 8 CFU/day for determination of gastrointestinal colonization and immune modulation (Valeur et al., 2004) Contributors: DCE, CNL and KFM wrote the protocol. DCE performed the study. CNL did the microbiological analysis of fecal samples and study product. EB performed the fluorescent whole cell hybridization of BB-12 like colonies. MB performed the PFGE of CRL-431 like colonies. PK performed the statistical analysis. CNL and SN wrote the first draft of the paper and all contributors participated in the revision and final approval of the paper.

show abstract

“…Furthermore, it has antioxidant properties and play a role in immune regulation, and may aid the protection against pathogenic bacteria and spoilage organisms, as well as assist in the conservation of the predominant gastrointestinal probiotic flora (Akalin et al, 1997;Abd El-Gawad et al, 2005;Nguyen et al, 2007;Mathara et al, 2008;Zheng et al, 2013). The objective of this study was to evaluate the effects of different doses of Kluyveromyces marxianus M3 yeast isolated from Tibetan mushrooms on plasma and egg yolkcholesterol contents, egg quality, and intestinal flora of the laying hens.…”

Section: Effects Of Kluyveromyces Marxianus Isolated From Tibetan Musmentioning

confidence: 99%

Effects of Kluyveromyces marxianus Isolated from Tibetan Mushrooms on the Plasma Lipids, Egg Cholesterol Level, Egg Quality and Intestinal Health of Laying Hens

Zhong

Liu

Zhang

et al. 2016

Rev. Bras. Cienc. Avic.

View full text Add to dashboard Cite

The effects of the Kluyveromyces marxianus M3 strain, isolated from Tibetan mushrooms, on plasma lipids, egg cholesterol level, egg quality, and intestinal health of laying hens were evaluated. In total, 160 Beijing fatty laying hens (43 weeks old) were divided into four groups and fed a basal diet supplemented with 0%, 0.1%, 0.3%, or 0.5% freeze-dried K. marxianus M3 powder for four weeks. The results showed that yeast supplementation reduced serum total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C), and very low density lipoprotein-cholesterol (VLDL-C) levels (p<0.01), and increased serum high density lipoprotein-cholesterol (HDL-C) level (p<0.05). Moreover, regardless of K. marxianus M3 dietary addition level, the cholesterol content of the eggs decreased by more than 26%. When0.3% yeast was supplemented, significant differences were found in the egg weights, shell strength, albumen height, Haugh unit and nutrient content of the eggs (p<0.01). Finally, 0.3% yeast supplementation improved the intestinal flora conditions of the hens by decreasing the Salmonella and Staphylococcus aureus counts (p<0.01) and increasing the Bifidobacterium count (p<0.01). The results in this work demonstrated that yeast culture supplementation to the diets deceased the serum and egg yolk cholesterol, and increased egg quality.

show abstract

The hypocholesterolaemic effect of milk yoghurt and soy-yoghurt containing bifidobacteria in rats fed on a cholesterol-enriched diet

Cited by 121 publications

References 26 publications

Lactobacillus acidophilus 74-2 and Bifidobacterium animalis subsp lactis DGCC 420 modulate unspecific cellular immune response in healthy adults

Lactobacillus acidophilus 74-2 and Bifidobacterium animalis subsp lactis DGCC 420 modulate unspecific cellular immune response in healthy adults

Dose–response study of probiotic bacteria Bifidobacterium animalis subsp lactis BB-12 and Lactobacillus paracasei subsp paracasei CRL-341 in healthy young adults

Effects of Kluyveromyces marxianus Isolated from Tibetan Mushrooms on the Plasma Lipids, Egg Cholesterol Level, Egg Quality and Intestinal Health of Laying Hens

Contact Info

Product

Resources

About