2012
DOI: 10.1016/j.febslet.2012.05.062
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The abp gene in Geobacillus stearothermophilus T‐6 encodes a GH27 β‐l‐arabinopyranosidase

Abstract: Edited by Miguel De la RosaKeywords: b-L-Arabinopyranosidase Glycoside hydrolase family 27 Arabinopyranose Sugar beet arabinan Larch arabinogalactan 13 C NMR a b s t r a c tIn this study we demonstrate that the abp gene in Geobacillus stearothermophilus T-6 encodes a family 27 glycoside hydrolase b-L-arabinopyranosidase. The catalytic constants towards the chromogenic substrate pNP-b-L-arabinopyranoside were 0.8 ± 0.1 mM, 6.6 ± 0.3 s À1 , and 8.2 ± 0.3 s À1 mM À1for K m , k cat and k cat /K m , respectively. 1… Show more

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Cited by 16 publications
(28 citation statements)
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“…As previously suggested (Salama et al, 2012) and further discussed below, Ile67 appeared to be a key residue for the substrate-binding and catalytic specificity of Abp. In order to test this hypothesis, this residue was replaced by aspartic acid, as briefly summarized in the following.…”
Section: Preparation and Characterization Of The Abp-i67d Mutantsupporting
confidence: 60%
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“…As previously suggested (Salama et al, 2012) and further discussed below, Ile67 appeared to be a key residue for the substrate-binding and catalytic specificity of Abp. In order to test this hypothesis, this residue was replaced by aspartic acid, as briefly summarized in the following.…”
Section: Preparation and Characterization Of The Abp-i67d Mutantsupporting
confidence: 60%
“…As mentioned above, Abp is a -l-arabinopyranosidase that removes -l-arabinopyranoside moieties from the side chains of l-arabinose-containing polysaccharides ( Fig. 1; Salama et al, 2012). Sequence-alignment analysis determined that the catalytic residues of Abp are Asp197 and Asp255, with Asp197 serving as the nucleophile and Asp255 as the acid/base for the reaction (Salama et al, 2012).…”
Section: The Active Sitementioning
confidence: 95%
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“…Thermophilic endospore-formers constitute an excellent resource of biotechnologically important products and can be used as the producers of these proteins [6][7][8][9][10]. It is essential to monitor the purity of the protein-producing strains in these processes.…”
Section: Introductionmentioning
confidence: 99%