The <i>Arabidopsis thaliana</i> ethylene‐responsive element binding protein (AtEBP) can function as a dominant suppressor of Bax‐induced cell death of yeast

Pan, Ling; Kawai, Maki; Yu, Liang; Kim, Kyung-Min; Hirata, Aiko; Umeda, Masaaki; Uchimiya, Hirofumi

doi:10.1016/s0014-5793(01)03098-8

Cited by 40 publications

(37 citation statements)

References 22 publications

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“…This approach is particularly useful in situations where functional screens are not experimentally well developed (e.g., plants) [27,[51][52][53]. For example, we recently reported that expression of animal antiapoptotic genes (e.g., Bcl-xL, CED-9, Op-IAP) in tobacco plants confers heritable resistance to several necrotrophic fungal and viral pathogens [54].…”

Section: Discussionmentioning

confidence: 99%

Bcl-2 family members inhibit oxidative stress-induced programmed cell death in Saccharomyces cerevisiae

Chen

Dunigan

Dickman

2003

Free Radical Biology and Medicine

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Section: Discussionmentioning

confidence: 99%

Bcl-2 family members inhibit oxidative stress-induced programmed cell death in Saccharomyces cerevisiae

Chen

Dunigan

Dickman

2003

Free Radical Biology and Medicine

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“…Direct regulators and executors of PCD in plants have also been identified by their PCD suppressing activity when they are overexpressed in animals, yeasts, and plants. These include an endoplasmic reticulumassociated BAX INHIBITOR-1 (BI-1; Kawai-Yamada et al, 2001;Watanabe and Lam, 2006;Ihara-Ohori et al, 2007), a transcription factor AtEBP (Pan et al, 2001;Ogawa et al, 2005), a vesicle-associated protein VAMP (Levine et al, 2001), and an AGC kinase Adi3 (Devarenne et al, 2006). These proteins possess a diverse variety of biochemical activities and localize to different cellular compartments, suggesting the involvement of many biochemical and cellular processes in regulating or executing PCD.…”

Section: Regulation Of Cell Death and Defense By The Bap And Bon Protmentioning

confidence: 99%

The ArabidopsisBAP1andBAP2Genes Are General Inhibitors of Programmed Cell Death

Yang

et al. 2007

Plant Physiology

103

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Here we identify the BAP1 and BAP2 genes of Arabidopsis (Arabidopsis thaliana) as general inhibitors of programmed cell death (PCD) across the kingdoms. These two homologous genes encode small proteins containing a calcium-dependent phospholipid-binding C2 domain. BAP1 and its functional partner BON1 have been shown to negatively regulate defense responses and a disease resistance gene SNC1. Genetic studies here reveal an overlapping function of the BAP1 and BAP2 genes in cell death control. The loss of BAP2 function induces accelerated hypersensitive responses but does not compromise plant growth or confer enhanced resistance to virulent bacterial or oomycete pathogens. The loss of both BAP1 and BAP2 confers seedling lethality mediated by PAD4 and EDS1, two regulators of cell death and defense responses. Overexpression of BAP1 or BAP2 with their partner BON1 inhibits PCD induced by pathogens, the proapototic gene BAX, and superoxide-generating paraquat in Arabidopsis or Nicotiana benthamiana. Moreover, expressing BAP1 or BAP2 in yeast (Saccharomyces cerevisiae) alleviates cell death induced by hydrogen peroxide. Thus, the BAP genes function as general negative regulators of PCD induced by biotic and abiotic stimuli including reactive oxygen species. The dual roles of BAP and BON genes in repressing defense responses mediated by disease resistance genes and in inhibiting general PCD has implications in understanding the evolution of plant innate immunity.

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“…Our finding that barley BI-1 is able to substitute for the cell death suppressor MLO as a suppressor of Bgh penetration resistance supports the idea that BI-1 inhibits a specific, although unidentified, type of endogenous plant PCD. Interestingly, besides BI-1, two antioxidants, a soybean ascorbate peroxidase and a tomato glutathione S-transferase, as well as nuclear AtEBP, a protein that is known to be ethylene responsive, are able to suppress BAX-induced cell death in yeast (32)(33)(34). Barley and Arabidopsis BI-1 genes are responsive to pathogen challenge and wounding, which are both associated with oxidative stress.…”

Section: Figmentioning

confidence: 99%

Overexpression of barley BAX inhibitor 1 induces breakdown of mlo -mediated penetration resistance to Blumeria graminis

Hückelhoven

Dechert²,

Kögel³

2003

Proc. Natl. Acad. Sci. U.S.A.

175

137

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Cell death regulation is linked to pathogen defense in plants and animals. Execution of apoptosis as one type of programmed cell death in animals is irreversibly triggered by cytochrome c release from mitochondria via pores formed by BAX proteins. This type of programmed cell death can be prevented by expression of BAX inhibitor 1 (BI-1), a membrane protein that protects cells from the effects of BAX by an unknown mechanism. In barley, a homologue of the mammalian BI-1 is expressed in response to inoculation with the barley powdery mildew fungus Blumeria graminis f.sp. hordei (Bgh). We found differential expression of BI-1 in response to Bgh in susceptible and resistant plants. Chemical induction of resistance to Bgh by soil drench treatment with 2,6-dichloroisonicotinic acid led to down-regulation of the expression level of BI-1. Importantly, single-cell transient overexpression of BI-1 in epidermal leaf tissue of susceptible barley cultivar Ingrid led to enhanced accessibility, resulting in a higher penetration efficiency of Bgh on BI-1-transformed cells. In Bgh-resistant mlo5 genotypes, which do not express the negative regulator of defense and cell death MLO, overexpression of BI-1 almost completely reconstituted susceptibility to fungal penetration. We suggest that BI-1 is a regulator of cellular defense in barley sufficient to substitute for MLO function in accessibility to fungal parasites.

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The Arabidopsis thaliana ethylene‐responsive element binding protein (AtEBP) can function as a dominant suppressor of Bax‐induced cell death of yeast

Cited by 40 publications

References 22 publications

Bcl-2 family members inhibit oxidative stress-induced programmed cell death in Saccharomyces cerevisiae

Bcl-2 family members inhibit oxidative stress-induced programmed cell death in Saccharomyces cerevisiae

The ArabidopsisBAP1andBAP2Genes Are General Inhibitors of Programmed Cell Death

Overexpression of barley BAX inhibitor 1 induces breakdown of mlo -mediated penetration resistance to Blumeria graminis

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