The<i>Arabidopsis thaliana</i>STYLISH1 Protein Acts as a Transcriptional Activator Regulating Auxin Biosynthesis

Eklund, D. Magnus; Ståldal, Veronika; Valsecchi, Isabel; Cierlik, Izabela; Eriksson, C.J. Peter; Hiratsu, Keiichiro; Ohme‐Takagi, Masaru; Sundström, Jens F.; Thelander, Mattias; Ezcurra, Inés; Sundberg, Eva

doi:10.1105/tpc.108.064816

Cited by 166 publications

(179 citation statements)

References 56 publications

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“…These were dominated by genes involved in auxin signaling and brassinosteroid biosynthesis. In addition, two of the putative EBB1 gene targets encode SHORT INTERNODE RELATED SEQUEN-CES (SRS), which are transcription factors that have been implicated in regulation of auxin biosynthesis in Arabidopsis through activation of the YUCCA gene (41,42). In support of our findings, recent evidence suggests that auxin is important for reinitiating growth after dormancy (43).…”

Section: Discussionsupporting

confidence: 76%

EARLY BUD-BREAK 1 (EBB1) is a regulator of release from seasonal dormancy in poplar trees

Yordanov

Strauss

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

139

119

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Trees from temperate latitudes transition between growth and dormancy to survive dehydration and freezing stress during winter months. We used activation tagging to isolate a dominant mutation affecting release from dormancy and identified the corresponding gene EARLY BUD-BREAK 1 (EBB1). We demonstrate through positioning of the tag, expression analysis, and retransformation experiments that EBB1 encodes a putative APETALA2/Ethylene responsive factor transcription factor. Transgenic up-regulation of the gene caused early bud-flush, whereas down-regulation delayed bud-break. Native EBB1 expression was highest in actively growing apices, undetectable during the dormancy period, but rapidly increased before bud-break. The EBB1 transcript was localized in the L1/L2 layers of the shoot meristem and leaf primordia. EBB1-overexpressing transgenic plants displayed enlarged shoot meristems, open and poorly differentiated buds, and a higher rate of cell division in the apex. Transcriptome analyses of the EBB1 transgenics identified 971 differentially expressed genes whose expression correlated with the EBB1 expression changes in the transgenic plants. Promoter analysis among the differentially expressed genes for the presence of a canonical EBB1-binding site identified 65 putative target genes, indicative of a broad regulatory context of EBB1 function. Our results suggest that EBB1 has a major and integrative role in reactivation of meristem activity after winter dormancy.adaptation | phenology | regeneration | climate change

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Section: Discussionsupporting

confidence: 76%

EARLY BUD-BREAK 1 (EBB1) is a regulator of release from seasonal dormancy in poplar trees

Yordanov

Strauss

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

139

119

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“…We recently showed that SHORT INTERNODES/ STYLISH (SHI/STY) family members are important throughout plant development and directly regulate YUC4-mediated auxin biosynthesis in Arabidopsis Ståldal et al, 2008;Eklund et al, 2010a), and we could show that transcription of YUC8 was activated by STY1 (Eklund et al, 2010a). This suggests that the temporal and spatial regulation of SHI/STY family members may be crucial for the developmental regulation of auxin production.…”

mentioning

confidence: 56%

“…The carpel valve length is significantly reduced in plants with a reduced level of SHI/STY gene activity, such as the SHI/STY quintuple mutant, and in plants expressing a STY1 protein transformed from a transcriptional activator to a repressor by the addition of a C-terminal SRDX tag Eklund et al, 2010a). Interestingly, it was recently demonstrated that around 25% of the drn-1 drnl-1 flowers have one or two shortened carpel valves and/or are missing one valve (Chandler et al, 2011b; Table II).…”

Section: Loss Of Drn Drnl and Puchi Functions Results In Reduced Stmentioning

confidence: 99%

Expression of ArabidopsisSHORT INTERNODES/STYLISHFamily Genes in Auxin Biosynthesis Zones of Aerial Organs Is Dependent on a GCC Box-Like Regulatory Element

et al. 2011

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Auxin/indole-3-acetic acid (IAA) biosynthesis in Arabidopsis (Arabidopsis thaliana) plays a major role in growth responses to developmental and genetic signals as well as to environmental stimuli. Knowledge of its regulation, however, remains rudimentary, and few proteins acting as transcriptional modulators of auxin biosynthesis have been identified. We have previously shown that alteration in the expression level of the SHORT INTERNODES/STYLISH (SHI/STY) family member STY1 affects IAA biosynthesis rates and IAA levels and that STY1 acts as a transcriptional activator of genes encoding auxin biosynthesis enzymes. Here, we have analyzed the upstream regulation of SHI/STY family members to gain further insight into transcriptional regulation of auxin biosynthesis. We attempted to modulate the normal expression pattern of STY1 by mutating a putative regulatory element, a GCC box, located in the proximal promoter region and conserved in most SHI/STY genes in Arabidopsis. Mutations in the GCC box abolish expression in aerial organs of the adult plant. We also show that induction of the transcriptional activator DORNRÖ SCHEN-LIKE (DRNL) activates the transcription of STY1 and other SHI/STY family members and that this activation is dependent on a functional GCC box. Additionally, STY1 expression in the strong drnl-2 mutant or the drn drnl-1 puchi-1 triple mutant, carrying knockdown mutations in both DRNL and its close paralogue DRN as well as one of their closest homologs, PUCHI, was significantly reduced, suggesting that DRNL regulates STY1 during normal plant development and that several other genes might have redundant functions.

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“…S4). Utilization of chimeric repressors is an established and effective approach to investigating the loss of function phenotypes of transcription activators when they have a degree of functional redundancy with other related proteins [37][38][39][40][41] . Transgenic Arabidopsis lines expressing NLP6-SUPRD were generated by transformation of the 4 Â NRE-min-GUS line 28 (WT in Fig.…”

Section: Resultsmentioning

confidence: 99%

Arabidopsis NIN-like transcription factors have a central role in nitrate signalling

2013

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In plants, nitrate is not only a major nitrogen source but also a signalling molecule that modulates the expression of a wide range of genes and that regulates growth and development. The critical role of nitrate as a signalling molecule has been established for several decades. However, the molecular mechanisms underlying the nitrate response have remained elusive, as the transcription factor that primarily responds to nitrate signals has not yet been identified. Here we show that Arabidopsis NIN-LIKE PROTEIN (NLP) family proteins bind the nitrate-responsive cis-element and activate nitrate-responsive cis-element-dependent and nitrate-responsive transcription. Our results also suggest that the activity of NLPs is posttranslationally modulated by nitrate signalling. Furthermore, the suppression of NLP function impairs the nitrate-inducible expression of a number of genes and causes severe growth inhibition. These results indicate that NLPs are the transcription factors mediating the nitrate signal and thereby function as master regulators of the nitrate response.

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TheArabidopsis thalianaSTYLISH1 Protein Acts as a Transcriptional Activator Regulating Auxin Biosynthesis

Cited by 166 publications

References 56 publications

EARLY BUD-BREAK 1 (EBB1) is a regulator of release from seasonal dormancy in poplar trees

EARLY BUD-BREAK 1 (EBB1) is a regulator of release from seasonal dormancy in poplar trees

Expression of ArabidopsisSHORT INTERNODES/STYLISHFamily Genes in Auxin Biosynthesis Zones of Aerial Organs Is Dependent on a GCC Box-Like Regulatory Element

Arabidopsis NIN-like transcription factors have a central role in nitrate signalling

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