The <i>Bacillus subtilis</i> cytochrome‐<i>c</i> oxidase

Saraste, Matti; Metso, Tuula; Nakari, Tiina; Jalli, Tuulikki; Lauraeus, Marko; Oost, John van der

doi:10.1111/j.1432-1033.1991.tb15732.x

Cited by 123 publications

(59 citation statements)

References 39 publications

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“…A sequence alignment. The sequences of subunits I and 111 of the the haem-containing copper oxidases from E. coli (CyoB and CyoC; Chepuri et al, 1990) and Bacillus subtilis (CtaC and CtaD;Saraste et al, 1991b) are aligned with SoxM. Putative transmembrane regions are hatched (note that CyoB has an extra segment labelled TMO at it N-terminus), and amino acids which are invariant in all known haem-containing copper oxidases are boxed.…”

Section: Soxm Purifies With Cytochrome B Soxc and A Rieske Fe-s Cementioning

confidence: 99%

A Second Terminal Oxidase in Sulfolobus acidocaldarius

Lübben

Arnaud

Castresana

et al. 1994

European Journal of Biochemistry

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We previously found that the soxABCD operon encodes a quinol oxidase complex in Sulfolobus acidocaldarius and this enzyme was purified and characterized. In this study, we have used a cloning procedure based on the conservation of oxidase sequences and the polymerase chain reaction to isolate a new gene (soxM) encoding a subunit of another terminal oxidase. This terminal oxidase is a fusion between two central components of cytochrome oxidases, subunits I and III. soxM forms a transcriptional unit which is expressed under heterotrophic growth conditions. The corresponding protein was detected by direct protein sequencing in a preparation enriched with a cytochrome absorbing light at 562 nm. This preparation contains a terminal oxidase which is able to oxidize the artificial substrate N,N,N′,N′ ‐tetramethyl‐p ‐phenylenediamine. This preparation also contains SoxC, a protein homologous to the mitochondrial cytochrome b, and a Rieske iron‐sulphur center. We suggest that SoxM is the core component of a second terminal oxidase complex and that this complex may share a subunit (SoxC) with the SoxABCD complex.

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Section: Soxm Purifies With Cytochrome B Soxc and A Rieske Fe-s Cementioning

confidence: 99%

A Second Terminal Oxidase in Sulfolobus acidocaldarius

Lübben

Arnaud

Castresana

et al. 1994

European Journal of Biochemistry

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“…A water soluble CuA domain was generated using the polymerase chain reaction (PCR), with as a template the genomic B. subtilis clone i110, carrying the eta gene cluster [12]. In this construct the ATG start codon has been introduced as a NcoI site (underlined) in the aminoterminal primer (no.…”

Section: Construction and Expressionmentioning

confidence: 99%

“…1) [12]. Here we report the cloning and expression of the Cu, domain of B. subtilis CtaC in E. coli.…”

Section: Introductionmentioning

confidence: 99%

The Cu_Asite of the caa₃‐type oxidase of Bacillus subtilis is a mixed‐valence binuclear copper centre

Wachenfeldt¹,

Vries²,

Oost³

1994

FEBS Letters

101

106

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A copper-containing domain of the caa,-type oxidase from Bacillus subtilis has been expressed as a water-soluble protein in the cytoplasm of Escherichia coli. Electron paramagnetic resonance (EPR) spectra of this purple domain show well-resolved lines in the g, resonance, both at X-band and S-band frequencies. Interpretation of EPR spectra and analytical data indicate a binuclear copper site consisting of one Cu*' and one Cu". This copper site closely resembles Cu, in subunit II of cytochrome c oxidase and is shown here to be a mixed-valence [Cu'+-Cu'+] binuclear centre.

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“…The caa 3 -type COX is expressed during growth on nonfermentable substrates such as succinate (7). It is also a typical proton-pumping hemecopper oxidase and is encoded by the ctaCDEF genes (15). Cytochrome bd can be found in B. subtilis cells grown with glucose (11,16,19) and is a QOX that does not pump protons (11) (see also Refs.…”

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A Cytochrome bb′-type Quinol Oxidase inBacillus subtilis Strain 168

Azarkina¹,

Siletsky²,

Borisov³

et al. 1999

Journal of Biological Chemistry

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The aerobic respiratory system of Bacillus subtilis 168 is known to contain three terminal oxidases: cytochrome caa 3 , which is a cytochrome c oxidase, and cytochrome aa 3 and bd, which are quinol oxidases. The presence of a possible fourth oxidase in the bacterium was investigated using a constructed mutant, LUH27, that lacks the aa 3 and caa 3 terminal oxidases and is also deficient in succinate:menaquinone oxidoreductase. The cytochrome bd content of LUH27 can be varied by using different growth conditions. LUH27 membranes virtually devoid of cytochrome bd respired with NADH or exogenous quinol as actively as preparations containing 0.4 nmol of cytochrome bd/mg of protein but were more sensitive to cyanide and aurachin D. The reduced minus oxidized difference spectra of the bd-deficient membranes as well as absorption changes induced by CO and cyanide indicated the presence of a "cytochrome o"-like component; however, the membranes did not contain heme O. The results provide strong evidence for the presence of a terminal oxidase of the bb type in B. subtilis. The enzyme does not pump protons and combines with CO much faster than typical heme-copper oxidases; in these respects, it resembles a cytochrome bd rather than members of the heme-copper oxidase superfamily. The genome sequence of B. subtilis 168 contains gene clusters for four respiratory oxidases. Two of these clusters, cta and qox, are deleted in LUH27. The remaining two, cydAB and ythAB, encode the identified cytochrome bd and a putative second cytochrome bd, respectively. Deletion of ythAB in strain LUH27 or the presence of the yth genes on plasmid did not affect the expression of the bb oxidase. It is concluded that the novel bb-type oxidase probably is cytochrome bd encoded by the cyd locus but with heme D being substituted by high spin heme B at the oxygen reactive site, i.e. cytochrome b 558 b 595 b.

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The Bacillus subtilis cytochrome‐c oxidase

Cited by 123 publications

References 39 publications

A Second Terminal Oxidase in Sulfolobus acidocaldarius

A Second Terminal Oxidase in Sulfolobus acidocaldarius

The Cu_Asite of the caa₃‐type oxidase of Bacillus subtilis is a mixed‐valence binuclear copper centre

A Cytochrome bb′-type Quinol Oxidase inBacillus subtilis Strain 168

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The Bacillus subtilis cytochrome‐c oxidase

Cited by 123 publications

References 39 publications

A Second Terminal Oxidase in Sulfolobus acidocaldarius

A Second Terminal Oxidase in Sulfolobus acidocaldarius

The CuAsite of the caa3‐type oxidase of Bacillus subtilis is a mixed‐valence binuclear copper centre

A Cytochrome bb′-type Quinol Oxidase inBacillus subtilis Strain 168

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The Cu_Asite of the caa₃‐type oxidase of Bacillus subtilis is a mixed‐valence binuclear copper centre