2003
DOI: 10.1242/dev.00807
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TheCaenorhabditis elegansnonmuscle myosin genesnmy-1andnmy-2function as redundant components of thelet-502/Rho-binding kinase andmel-11/myosin phosphatase pathway during embryonic morphogenesis

Abstract: Rho-binding kinase and the myosin phosphatase targeting subunit regulate nonmuscle contractile events in higher eukaryotes. Genetic evidence indicates that the C. elegans homologs regulate embryonic morphogenesis by controlling the actin-mediated epidermal cell shape changes that transform the spherical embryo into a long, thin worm. LET-502/Rho-binding kinase triggers elongation while MEL-11/myosin phosphatase targeting subunit inhibits this contractile event. We describe mutations in the nonmuscle myosin hea… Show more

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Cited by 92 publications
(156 citation statements)
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“…Furthermore, actin foci were observed surrounding the germ nuclei. nmy-2, encoding the nonmuscle myosin II, displays the same expression pattern as actin filaments in the wildtype germline (Guo and Kemphues 1996;Piekny et al 2003). Consistently, NMY-2TGFP showed abnormal accumulation as actin filaments in the fli-1 mutant germline (Figure 10,Q and R).…”
Section: Resultsmentioning
confidence: 59%
“…Furthermore, actin foci were observed surrounding the germ nuclei. nmy-2, encoding the nonmuscle myosin II, displays the same expression pattern as actin filaments in the wildtype germline (Guo and Kemphues 1996;Piekny et al 2003). Consistently, NMY-2TGFP showed abnormal accumulation as actin filaments in the fli-1 mutant germline (Figure 10,Q and R).…”
Section: Resultsmentioning
confidence: 59%
“…Among the seven candidates retained (see Table S1 in the supplementary material), we characterized T12D8.6, the knockdown of which induced a strong elongation defect very similar to that observed in embryos lacking the two myosin heavy chains NMY-1 and NMY-2 (Piekny et al, 2003). We named T12D8.6 mlc-5, as its predicted translation product displays strong homology (45% and 55% identity over its EF-hand domains) (Fig.…”
Section: Identification Of a New Essential Myosin Light Chainmentioning
confidence: 96%
“…Partial RNAi knockdown of MLC-4 (Fig. 3F) or of the myosin heavy chain NMY-2 in the nmy-1 mutant background bypasses their early requirement for cytokinesis and leads to elongation arrest at the 1.2-fold stage (Piekny et al, 2003). We assumed that this phenotype reflects what should be observed when non-muscle myosin II is inactive, with essentially no maternal contribution specifically during elongation.…”
Section: Rnai Screens Looking For Regulators or Components Of The Nonmentioning
confidence: 97%
See 1 more Smart Citation
“…The following primary antibodies and antisera were used: rabbit anti-GFP (ab6556, Abcam), anti-UNC-52 (Mullen et al, 1999), anti-LET-2 , rat anti-NMY-1 (Piekny et al, 2003), mouse anti-PAR-3 (Nance et al, 2003), MH25 (Francis and Waterston, 1985), mAbGJ1 (anti-LAM-3), mAbGJ2 (pan-laminin) (Rasmussen et al, 2008), IFA (Pruss et al, 1981) and mAbPL1 (G. Hermann and J.R.P., unpublished). For quantification of centrosome orientation, image stacks were acquired of PHA-4::GFP-expressing embryos immunostained with GFP and IFA antibodies (a marker of centrosomes) (Leung et al, 1999).…”
Section: Immunofluorescencementioning
confidence: 99%