The cluA ⁻ mutant of Dictyostelium identifies a novel class of proteins required for dispersion of mitochondria

Abstract

The

FRIENDLY Regulates Mitochondrial Distribution, Fusion, and Quality Control in Arabidopsis

“…Here, we provide new insights into the effects of mitochondrial clustering induced by mutation of the Arabidopsis CLU-type FRIENDLY gene and evidence for the role of this gene in regulating mitochondrial association and fusion. Work on Dictyostelium discoideum first identified the involvement of FRIENDLY-like (CLU-type) genes in the maintenance of mitochondrial distribution (Zhu et al, 1997). As a result of TEM analysis of clusters in the D. discoideum cluA 2 mutant showing continuity of the outer mitochondrial membrane of adjacent mitochondria, and sometimes penetration of the inner membrane between such connected mitochondria, it was concluded that CluA was involved in mitochondrial division (Fields et al, 2002).…”

Regulation of Dictyostelium Protein-tyrosine Phosphatase-3 (PTP3) through Osmotic Shock and Stress Stimulation and Identification of pp130 as a PTP3 Substrate

“…We excluded the possibility that these dotlike structures are mitochondria by transforming the Myctagged PTP3(WT) into the cluA null strain (23). In this strain, all mitochondria are clustered near the cell center (23). After 30 min stimulation with growth medium, the mitochondria, as visualized by immunostaining the mitochondrial protein F 1 B, were found localized near the center of the cell (Fig.…”

“…The partial ptp3 null strain, lacking one of the PTP3 genes, has been described (22). For mitochondrial localization studies, PTP3 overexpression constructs were transformed into the cluA null mutant (23). Transformation and clonal selection was carried out as described earlier (22).…”

“…We excluded the possibility that these dotlike structures are mitochondria by transforming the Myctagged PTP3(WT) into the cluA null strain (23). In this strain, all mitochondria are clustered near the cell center (23).…”

Molecular Mechanisms and Physiological Significance of Organelle Interactions and Cooperation

“…In D. discoideum mitochondrial distribution is maintained by the protein CluA (Zhu et al, 1997), which has homologs across a variety of organisms, including Arabidopsis thaliana, Saccharomyces cerevisiae, and Drosophila melanogaster. In all organisms studied to date, the absence of this protein results in clustered mitochondria (Zhu et al, 1997;Fields et al, 1998;Logan et al, 2003;Cox and Spradling, 2009).…”