The <i>Drosophila</i> G protein‐coupled receptor, Methuselah, exhibits a promiscuous response to peptides

Ja, William W.; Carvalho, Gil B.; Madrigal, Marisol; Roberts, Richard W.; Benzer, Seymour

doi:10.1002/pro.221

Cited by 35 publications

(26 citation statements)

References 22 publications

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“…2), a combination that is typical for position 2/3 of only the type I turn [26]. On the other hand, for SP dissolved in water only two weak medium range NOEs are seen between Trp 3 and Trp 5 . This suggests that a nascent rather than a stable turn is populated in this region in pure water.…”

Section: Resultsmentioning

confidence: 92%

NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster

2011

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a b s t r a c tThe insect sex peptide (SP) elicits a variety of biological responses upon transfer to the mated female. SP contains 36 amino acids, including a tryptophan-rich N-terminal region, a central region containing five hydroxyproline (Hyp) residues, and a C-terminal region enclosed by a disulfide bridge. The solution structure of SP, studied here using NMR spectroscopy, includes a motif WPWN that adopts a type I b-turn in the N-terminal Trp-rich region. This turn region is connected to the central Hyp-rich region, which adopts extended and/or PPII-like conformations. The C-terminal disulfide-bonded loop populates helical turns or nascent helical structure. Overall, the results reveal a rather flexible peptide that lacks a compact folded structure in solution.

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Section: Resultsmentioning

confidence: 92%

NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster

2011

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“…RT-qPCR was performed using QScript XLT 1-step RT-qPCR ToughMix (Quanta Biosciences) on a CFX96 Touch RealTime PCR Detection system (Bio-Rad) with a commercially designed primer and probe assay (TaqMan Gene Expression Assays, Life Technologies). Alternatively, first-strand cDNA was synthesized with random nonamer primers before qPCR using iQ SYBR Green Supermix (Bio-Rad), as described previously (66), and predefined primer pairs from FlyPrimerBank (67). Relative gene expression was calculated using the ΔΔCt method (68) and normalized to total input RNA.…”

Section: Methodsmentioning

confidence: 99%

The 4E-BP growth pathway regulates the effect of ambient temperature on Drosophila metabolism and lifespan

Carvalho

Drago

Hoxha

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Changes in body temperature can profoundly affect survival. The dramatic longevity-enhancing effect of cold has long been known in organisms ranging from invertebrates to mammals, yet the underlying mechanisms have only recently begun to be uncovered. In the nematode Caenorhabditis elegans, this process is regulated by a thermosensitive membrane TRP channel and the DAF-16/FOXO transcription factor, but in more complex organisms the underpinnings of cold-induced longevity remain largely mysterious. We report that, in Drosophila melanogaster, variation in ambient temperature triggers metabolic changes in protein translation, mitochondrial protein synthesis, and posttranslational regulation of the translation repressor, 4E-BP (eukaryotic translation initiation factor 4E-binding protein). We show that 4E-BP determines Drosophila lifespan in the context of temperature changes, revealing a genetic mechanism for cold-induced longevity in this model organism. Our results suggest that the 4E-BP pathway, chiefly thought of as a nutrient sensor, may represent a master metabolic switch responding to diverse environmental factors.S tudies on the biological underpinnings of aging have uncovered numerous genetic and environmental factors regulating animal lifespan. Longevity-promoting genes include components of the insulin-like signaling pathway, the histone deacetylase Sir2, the GTPase Ras, TRP membrane channels, and transcription factors, among many others (1, 2). Environmental manipulations extending life include changes in nutrition (often referred to as caloric or dietary restriction), sexual/reproductive history, and ambient temperature (3-5).Of the factors known to impact aging, temperature is arguably the most promising. Lifespan extension by cold is evolutionarily conserved-documented in poikilotherms, such as worms and flies, and homeotherms, including mammals (4, 6-11)-and more robust than well-studied interventions such as dietary restriction (12, 13). However, the underlying mechanisms remain incompletely understood (10,14,15). In Caenorhabditis elegans, the effect of cold on survival involves the thermosensitive membrane channel TRPA-1 acting upstream of the DAF-16/FOXO transcription factor (14,15). This seminal finding countered the notion that longevity is the passive result of general thermodynamic changes, and favored instead the view that genetic pathways actively control lifespan in response to ambient temperature. However, these results have to date not been extended or replicated in other model organisms. Explanatory theories for the effect of cold on longevity include a reduction in reactive oxygen species generated by mitochondrial uncoupling proteins, suppression of autoimmune response, and changes in neuroendocrine factors (6,16,17), but these views remain largely speculative given the lack of further mechanistic insight into lifespan extension by cold in model organisms.We report that, in Drosophila, changes in temperature trigger a metabolic program impacting protein translation, mitochondrial prot...

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“…The ortholog of SPR in Helicoverpa armigera is also essential for female postmating responses such as pheromone production (Fan et al 1999; Hanin et al 2012). Recent studies suggest that there may be additional receptors for SP, and that SPR may facilitate access of SP to the nervous system (Ja et al 2009; Haussmann et al 2013). Additional female-encoded molecules have been identified that are also essential for SP effects (including on receptivity [Findlay et al 2014]).…”

Section: Do Sfps Contribute To Postmating Sexual Conflict?mentioning

confidence: 99%

Sexual Conflict and Seminal Fluid Proteins: A Dynamic Landscape of Sexual Interactions

Sirot

Wong

Chapman

et al. 2014

Cold Spring Harb Perspect Biol

156

221

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Sexual reproduction requires coordinated contributions from both sexes to proceed efficiently. However, the reproductive strategies that the sexes adopt often have the potential to give rise to sexual conflict because they can result in divergent, sex-specific costs and benefits. These conflicts can occur at many levels, from molecular to behavioral. Here, we consider sexual conflict mediated through the actions of seminal fluid proteins. These proteins provide many excellent examples in which to trace the operation of sexual conflict from molecules through to behavior. Seminal fluid proteins are made by males and provided to females during mating. As agents that can modulate egg production at several steps, as well as reproductive behavior, sperm “management,” and female feeding, activity, and longevity, the actions of seminal proteins are prime targets for sexual conflict. We review these actions in the context of sexual conflict. We discuss genomic signatures in seminal protein (and related) genes that are consistent with current or previous sexual conflict. Finally, we note promising areas for future study and highlight real-world practical situations that will benefit from understanding the nature of sexual conflicts mediated by seminal proteins.

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The Drosophila G protein‐coupled receptor, Methuselah, exhibits a promiscuous response to peptides

Cited by 35 publications

References 22 publications

NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster

NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster

The 4E-BP growth pathway regulates the effect of ambient temperature on Drosophila metabolism and lifespan

Sexual Conflict and Seminal Fluid Proteins: A Dynamic Landscape of Sexual Interactions

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