The<i>DUET</i>gene is necessary for chromosome organization and progression during male meiosis in<i>Arabidopsis</i>and encodes a PHD finger protein

Reddy, Thamalampudi Venkata; Kaur, Jagreet; Agashe, Bhavna; Sundaresan, Venkatesan; Siddiqi, Imran

doi:10.1242/dev.00827

Cited by 71 publications

(75 citation statements)

References 61 publications

(66 reference statements)

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“…The chromosomal phenotypes first become apparent at postpachytene stages. Other Arabidopsis genes and mutants affecting meiosis for which defects are mainly seen after pachytene are dsy1 (Ross et al, 1997), dsy10 (Cai and Makaroff, 2001), mei1 (He et al, 1996;Grelon et al, 2003), mmd1/duet (Reddy et al, 2003;Yang et al, 2003), At CAPE1, At CAPE2 (Siddiqui et al, 2003), and At CDC45 (Stevens et al, 2004). Several of these encode proteins that are directly implicated in chromatin organization and include proteins that are considered to act in mitosis as well as meiosis.…”

Section: Discussionmentioning

confidence: 99%

TheArabidopsis-mei2-LikeGenes Play a Role in Meiosis and Vegetative Growth inArabidopsis

2006

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The Arabidopsis-mei2-Like (AML) genes comprise a five-member gene family related to the mei2 gene, which is a master regulator of meiosis in Schizosaccharomyces pombe and encodes an RNA binding protein. We have analyzed the AML genes to assess their role in plant meiosis and development. All five AML genes were expressed in both vegetative and reproductive tissues. Analysis of AML1-AML5 expression at the cellular level indicated a closely similar expression pattern. In the inflorescence, expression was concentrated in the shoot apical meristem, young buds, and reproductive organ primordia. Within the reproductive organs, strong expression was observed in meiocytes and developing gametes. Functional analysis using RNA interference (RNAi) and combinations of insertion alleles revealed a role for the AML genes in meiosis, with RNAi lines and specific multiple mutant combinations displaying sterility and a range of defects in meiotic chromosome behavior. Defects in seedling growth were also observed at low penetrance. These results indicate that the AML genes play a role in meiosis as well as in vegetative growth and reveal conservation in the genetic mechanisms controlling meiosis in yeast and plants.

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Section: Discussionmentioning

confidence: 99%

TheArabidopsis-mei2-LikeGenes Play a Role in Meiosis and Vegetative Growth inArabidopsis

2006

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“…In Arabidopsis, there are three homologous genes (At1g33420, At1g66170, and At2g01810) to MS1 (At5g22260). At1g66170 is the MALE MEIO-CYTE DEATH1 (MMD1)/DUET gene and is thought to be a crucial regulator of meiosis (Reddy et al, 2003;Yang et al, 2003). The other two genes are not fully redundant with MS1 or MMD1/DUET, We used publicly available NASCArray data (experiment ID: NASCARRAYS-23) at http://affymetrix.Arabidopsis.info/.…”

Section: Phylogenetic Analysis Of the Ms1 Family And Phenotype Of Promentioning

confidence: 99%

Arabidopsis MALE STERILITY1 Encodes a PHD-Type Transcription Factor and Regulates Pollen and Tapetum Development

et al. 2007

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The Arabidopsis thaliana MALE STERILITY1 (MS1) gene encodes a nuclear protein with Leu zipper-like and PHD-finger motifs and is important for postmeiotic pollen development. Here, we examined MS1 function using both cell biological and molecular biological approaches. We introduced a fusion construct of MS1 and a transcriptional repression domain (MS1-SRDX) into wildtype Arabidopsis, and the transgenic plants showed a semisterile phenotype similar to that of ms1. Since the repression domain can convert various kinds of transcriptional activators to dominant repressors, this suggested that MS1 functioned as a transcriptional activator. The Leu zipper-like region and the PHD motif were required for the MS1 function. Phenotypic analysis of the ms1 mutant and the MS1-SRDX transgenic Arabidopsis indicated that MS1 was involved in formation of pollen exine and pollen cytosolic components as well as tapetum development. Next, we searched for MS1 downstream genes by analyzing publicly available microarray data and identified 95 genes affected by MS1. Using a transgenic ms1 plant showing dexamethasoneinducible recovery of fertility, we further examined whether these genes were immediately downstream of MS1. From these results, we discuss a role of MS1 in pollen and tapetum development and the conservation of MS1 function in flowering plants.

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“…Genetic analyses have led to the isolation of several mutants with distinct defects in stamen development (Dawson et al, 1993;Sanders et al, 1999;Schiefthaler et al, 1999;Yang et al, 1999;Canales et al, 2002;Zhao et al, 2002;Reddy et al, 2003;Sorensen et al, 2003;Steiner-Lange et al, 2003;Albrecht et al, 2005). Cloning of the genes affected in these mutants resulted in the identification of key regulators of stamen formation.…”

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Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

et al. 2007

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To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.

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TheDUETgene is necessary for chromosome organization and progression during male meiosis inArabidopsisand encodes a PHD finger protein

Cited by 71 publications

References 61 publications

TheArabidopsis-mei2-LikeGenes Play a Role in Meiosis and Vegetative Growth inArabidopsis

TheArabidopsis-mei2-LikeGenes Play a Role in Meiosis and Vegetative Growth inArabidopsis

Arabidopsis MALE STERILITY1 Encodes a PHD-Type Transcription Factor and Regulates Pollen and Tapetum Development

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

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