TheEscherichia coliorthologue of theSalmonella ushBgene (ushBc) produces neither UDP-sugar hydrolase activity nor detectable protein, but has an identical sequence to that ofEscherichia coli cdh

Schroder, Wayne A.; Burger, Michelle; Edwards, Catherine J.; Douglas, Meaghan; Innes, David J.; Beacham, Ifor R.; Burns, Dennis M.

doi:10.1111/j.1574-6968.2001.tb10821.x

Cited by 3 publications

(1 citation statement)

References 20 publications

(42 reference statements)

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“…Interestingly, whilst most S. enterica serotypes contain both activities, most serotype Typhimurium isolates contain an inactive (cryptic) ushA allele [5][6][7]. Likewise, E. coli K-12 and natural isolates of E. coli contain no detectable membrane-associated UDP-glucose hydrolase activity [1] due to an inactive allele of ushB [5,8].…”

Section: Introductionmentioning

confidence: 99%

Bacterial UDP-Glucose Hydrolases and P2 Receptor-Mediated Responses to Infection: A Commentary

Beacham¹,

Headrick²

2013

AID

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UDP-glucose hydrolases are a group of relatively little known membrane-bound or periplasmic enzymes found in Salmonella enterica and E. coli. UDP-glucose is an agonist for a specific P2 receptor (P2Y 14 ) found on epithelial cells and cells associated with innate immunity. It is also recognised as a 'danger signal'. Cells respond to mechanical damage by releasing UDP-glucose which activates P2Y 14 to trigger an innate immune response; it is postulated that a similar response to bacterial infection may be protective against infection. However, the UDP-glucose hydrolases may constitute virulence factors able to abrogate this response by degradation of the released UDP-glucose.

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Section: Introductionmentioning

confidence: 99%

Bacterial UDP-Glucose Hydrolases and P2 Receptor-Mediated Responses to Infection: A Commentary

Beacham¹,

Headrick²

2013

AID

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A challenge for 21st century molecular biology and biochemistry: what are the causes of obligate autotrophy and methanotrophy?

Wood¹,

Aurikko²,

Kelly³

2004

FEMS Microbiol Rev

163

113

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We assess the use to which bioinformatics in the form of bacterial genome sequences, functional gene probes and the protein sequence databases can be applied to hypotheses about obligate autotrophy in eubacteria. Obligate methanotrophy and obligate autotrophy among the chemo- and photo-lithotrophic bacteria lack satisfactory explanation a century or more after their discovery. Various causes of these phenomena have been suggested, which we review in the light of the information currently available. Among these suggestions is the absence in vivo of a functional alpha-ketoglutarate dehydrogenase. The advent of complete and partial genome sequences of diverse autotrophs, methylotrophs and methanotrophs makes it possible to probe the reasons for the absence of activity of this enzyme. We review the role and evolutionary origins of the Krebs cycle in relation to autotrophic metabolism and describe the use of in silico methods to probe the partial and complete genome sequences of a variety of obligate genera for genes encoding the subunits of the alpha-ketoglutarate dehydrogenase complex. Nitrosomonas europaea and Methylococcus capsulatus, which lack the functional enzyme, were found to contain the coding sequences for the E1 and E2 subunits of alpha-ketoglutarate dehydrogenase. Comparing the predicted physicochemical properties of the polypeptides coded by the genes confirmed the putative gene products were similar to the active alpha-ketoglutarate dehydrogenase subunits of heterotrophs. These obligate species are thus genomically competent with respect to this enzyme but are apparently incapable of producing a functional enzyme. Probing of the full and incomplete genomes of some cyanobacterial and methanogenic genera and Aquifex confirms or suggests the absence of the genes for at least one of the three components of the alpha-ketoglutarate dehydrogenase complex in these obligate organisms. It is recognized that absence of a single functional enzyme may not explain obligate autotrophy in all cases and may indeed be only be one of a number of controls that impose obligate metabolism. Availability of more genome sequences from obligate genera will enable assessment of whether obligate autotrophy is due to the absence of genes for a few or many steps in organic compound metabolism. This problem needs the technologies and mindsets of the present generation of molecular microbiologists to resolve it.

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Identification of new SdiA regulon members of Escherichia coli , Enterobacter cloacae , and Salmonella enterica serovars Typhimurium and Typhi

Schwieters,

Ahmer

2024

Microbiol Spectr

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Bacteria can coordinate behavior in response to population density through the production, release, and detection of small molecules, a phenomenon known as quorum sensing. Salmonella enterica is among a group of Enterobacteriaceae that can detect signaling molecules of the N -acyl homoserine lactone (AHL) type but lack the ability to produce them. The AHLs are detected by the LuxR-type transcription factor, SdiA. This enables a behavior known as eavesdropping, where organisms can sense the signaling molecules of other species of bacteria. The role of SdiA remains largely unknown. Here, we use RNA-seq to more completely identify the sdiA regulons of two clinically significant serovars of Salmonella enterica : Typhimurium and Typhi. We find that their sdiA regulons are largely conserved despite the significant differences in pathogenic strategy and host range of these two serovars. Previous studies identified sdiA -regulated genes in Escherichia coli and Enterobacter cloacae , but there is surprisingly no overlap in regulon membership between the different species. This led us to individually test orthologs of each regulon member in the other species and determine that there is indeed some overlap. Unfortunately, the functions of most sdiA -regulated genes are unknown, with the overall function of eavesdropping in these organisms remaining unclear. IMPORTANCE Many bacterial species detect their own population density through the production, release, and detection of small molecules (quorum sensing). Salmonella and other Enterobacteriaceae have a modified system that detects the N -acyl-homoserine lactones of other bacteria through the solo quorum sensing receptor SdiA, a behavior known as eavesdropping. The roles of sdiA -dependent eavesdropping in the lifecycles of these bacteria are unknown. In this study, we identify sdiA -dependent transcriptional responses in two clinically relevant serovars of Salmonella , Typhimurium and Typhi, and note that their responses are partially conserved. We also demonstrate for the first time that sdiA -dependent regulation of genes is partially conserved in Enterobacter cloacae and Escherichia coli as well, indicating a degree of commonality in eavesdropping among the Enterobacteriaceae.

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TheEscherichia coliorthologue of theSalmonella ushBgene (ushB^c) produces neither UDP-sugar hydrolase activity nor detectable protein, but has an identical sequence to that ofEscherichia coli cdh

Cited by 3 publications

References 20 publications

Bacterial UDP-Glucose Hydrolases and P2 Receptor-Mediated Responses to Infection: A Commentary

Bacterial UDP-Glucose Hydrolases and P2 Receptor-Mediated Responses to Infection: A Commentary

A challenge for 21st century molecular biology and biochemistry: what are the causes of obligate autotrophy and methanotrophy?

Identification of new SdiA regulon members of Escherichia coli , Enterobacter cloacae , and Salmonella enterica serovars Typhimurium and Typhi

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